##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AvrII BalI
BbeI BcnI BglI BlnI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FauI FseI
HaeII HgaI Hin1I KasI KpnI
KspI MluI MluNI MroI MscI
MslI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI Sse8387I StuI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AocI 168 CCTAAGG CC^T[ACGT]AGG
AspHI 3073 GAGCTC G[AT]GC[AT]^C
AspI 2691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 696 TGCGCA TGC^GCA
BanI 3384 GGTGCC G^G[CT][GA]CC
BbrPI 3347 CACGTG CAC^GTG
BbvCI 3360 GCTGAGG CCTCAGC(-5/-2)
BcgI 3630 CGATTACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 792 TGATCA T^GATCA
BglII 2265 AGATCT A^GATCT
BlpI 2635 GCTCAGC GC^T[ACGT]AGC
BpmI 2269 CTGGAG CTGGAG(16/14)
Bpu1102I 2635 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 3073 GAGCTC G[AT]GC[AT]^C
BsmBI 2543 CGTCTC CGTCTC(1/5)
BsrFI 1129 GCCGGT [GA]^CCGG[CT]
BstXI 3346 CCACGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 168 CCTAAGG CC^T[ACGT]AGG
CelII 2635 GCTCAGC GC^T[ACGT]AGC
CfoI 697 GCGC GCG^C
Cfr10I 1129 GCCGGT [GA]^CCGG[CT]
DsaI 2575 CCATGG C^C[GA][CT]GG
EaeI 2093 CGGCCA [CT]^GGCC[GA]
Eco81I 168 CCTAAGG CC^T[ACGT]AGG
EcoT14I 2575 CCATGG C^C[AT][AT]GG
FbaI 792 TGATCA T^GATCA
FspI 696 TGCGCA TGC^GCA
HapII 1130 CCGG C^CGG
HhaI 697 GCGC GCG^C
HinP1I 697 GCGC G^CGC
HindIII 2973 AAGCTT A^AGCTT
HpaI 2995 GTTAAC GTT^AAC
HpaII 1130 CCGG C^CGG
MspA1I 1277 CAGCTG C[AC]G^C[GT]G
MspI 1130 CCGG C^CGG
NcoI 2575 CCATGG C^CATGG
PflFI 2691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3347 CACGTG CAC^GTG
PmlI 3347 CACGTG CAC^GTG
PstI 2116 CTGCAG CTGCA^G
PvuII 1277 CAGCTG CAG^CTG
SacI 3073 GAGCTC GAGCT^C
ScaI 1390 AGTACT AGT^ACT
SfcI 2116 CTGCAG C^T[GA][CT]AG
SnaBI 2678 TACGTA TAC^GTA
SphI 3457 GCATGC GCATG^C
StyI 2575 CCATGG C^C[AT][AT]GG
Tth111I 2691 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 189 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 1342 CTTAAG C^TTAAG
2323 CTTAAG
AseI 3899 ATTAAT AT^TAAT
4616 ATTAAT
AsnI 3899 ATTAAT AT^TAAT
4616 ATTAAT
AvaII 512 GGACC G^G[AT]CC
1312 GGACC
BamHI 1014 GGATCC G^GATCC
2985 GGATCC
BanII 648 GGGCTC G[GA]GC[CT]^C
3073 GAGCTC
BfrI 1342 CTTAAG C^TTAAG
2323 CTTAAG
BmyI 648 GGGCTC G[AGT]GC[ACT]^C
3073 GAGCTC
BsaAI 2678 TACGTA [CT]AC^GT[GA]
3347 CACGTG
BsaBI 1699 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2668 GATAATGATC
BsaI 4007 GAGACC GGTCTC(1/5)
1743 GGTCTC
BsgI 2705 GTGCAG GTGCAG(16/14)
3600 GTGCAG
Bsp1286I 648 GGGCTC G[AGT]GC[ACT]^C
3073 GAGCTC
Cfr13I 512 GGACC G^G[ACGT]CC
1312 GGACC
DraII 511 AGGACCC [GA]G^G[ACGT]CC[CT]
1311 AGGACCT
EcoO109I 511 AGGACCC [GA]G^G[ACGT]CC[CT]
1311 AGGACCT
EcoRI 1819 GAATTC G^AATTC
4064 GAATTC
EcoRV 4562 GATATC GAT^ATC
4641 GATATC
HaeIII 2094 GGCC GG^CC
2765 GGCC
MaeII 2679 ACGT A^CGT
3348 ACGT
MamI 1699 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2668 GATAATGATC
MfeI 1286 CAATTG C^AATTG
3617 CAATTG
MunI 1286 CAATTG C^AATTG
3617 CAATTG
PpuMI 511 AGGACCC [GA]G^G[AT]CC[CT]
1311 AGGACCT
PshAI 273 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
471 GACGAATGTC
PshBI 3899 ATTAAT AT^TAAT
4616 ATTAAT
Sau96I 512 GGACC G^G[ACGT]CC
1312 GGACC
XcmI 1166 CCATTGATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2164 CCAGATGAAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I BbsI BbvI
BciVI BfaI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI ScrFI SfaNI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI