##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AgeI Aor51HI AscI
Asp718I AviII AvrII BbeI BbrPI
BcgI BglI BlnI BsaBI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstZ17I
Cfr10I ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoNI FseI
FspI HaeII KasI KpnI KspI
MamI NaeI NarI NgoMIV NheI
NotI NspV PaeR7I PinAI PmaCI
PmeI PmlI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SphI
Sse8387I XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 3581 GTAGAC GT^[AC][GT]AC
AclI 59 AACGTT AA^CGTT
AcyI 134 GACGCC G[GA]^CG[CT]C
AhdI 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1323 GTGCAC G^TGCAC
AlwNI 86 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4463 CCTGAGG CC^T[ACGT]AGG
ApaI 4419 GGGCCC GGGCC^C
ApaLI 1323 GTGCAC G^TGCAC
AspEI 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1509 CCCGGG C^[CT]CG[GA]G
BbvCI 1533 GCTGAGG CCTCAGC(-5/-2)
BciVI 3303 GTATCC GTATCC(6/5)
BclI 892 TGATCA T^GATCA
BglII 2500 AGATCT A^GATCT
BlpI 4713 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4713 GCTCAGC GC^T[ACGT]AGC
BsaAI 80 TACGTA [CT]AC^GT[GA]
BsaHI 134 GACGCC G[GA]^CG[CT]C
BsaI 2173 GAGACC GGTCTC(1/5)
BsoBI 1509 CCCGGG C^[CT]CG[GA]G
BsrDI 3432 GCAATG GCAATG(2/0)
BssSI 1239 CTCGTG CACGAG(-5/-1)
BstEII 714 GGTGACC G^GT[ACGT]ACC
BstPI 714 GGTGACC G^GT[ACGT]ACC
Bsu36I 4463 CCTGAGG CC^T[ACGT]AGG
CelII 4713 GCTCAGC GC^T[ACGT]AGC
Eam1105I 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4463 CCTGAGG CC^T[ACGT]AGG
EcoO65I 714 GGTGACC G^GT[ACGT]ACC
EcoRV 1202 GATATC GAT^ATC
FbaI 892 TGATCA T^GATCA
Hin1I 134 GACGCC G[GA]^CG[CT]C
HpaI 3536 GTTAAC GTT^AAC
MfeI 329 CAATTG C^AATTG
MluI 5077 ACGCGT A^CGCGT
MunI 329 CAATTG C^AATTG
NdeI 3469 CATATG CA^TATG
NruI 5373 TCGCGA TCG^CGA
PacI 1917 TTAATTAA TTAAT^TAA
PflFI 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4254 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1576 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 59 AACGTT AA^CGTT
ScaI 2762 AGTACT AGT^ACT
SexAI 710 ACCTGGT A^CC[AT]GGT
SmaI 1509 CCCGGG CCC^GGG
SnaBI 80 TACGTA TAC^GTA
SpeI 3130 ACTAGT A^CTAGT
StuI 1864 AGGCCT AGG^CCT
Tth111I 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4254 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5108 TCTAGA T^CTAGA
XmaCI 1509 CCCGGG C^CCGGG
XmaI 1509 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 5078 CGCG CG^CG
5374 CGCG
AccIII 1096 TCCGGA T^CCGGA
4771 TCCGGA
BamHI 3255 GGATCC G^GATCC
5379 GGATCC
BanI 2095 GGCACC G^G[CT][GA]CC
5516 GGCACC
BpmI 1835 CTCCAG CTGGAG(16/14)
272 CTGGAG
BsaWI 1096 TCCGGA [AT]^CCGG[AT]
4771 TCCGGA
BseAI 1096 TCCGGA T^CCGGA
4771 TCCGGA
BsgI 139 CTGCAC GTGCAG(16/14)
4374 CTGCAC
BspEI 1096 TCCGGA T^CCGGA
4771 TCCGGA
BstUI 5078 CGCG CG^CG
5374 CGCG
DrdI 3718 GACCTTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4259 GACTGGGTAGTC
EcoT22I 4733 ATGCAT ATGCA^T
5692 ATGCAT
FauI 1358 GCGGG CCCGC(4/6)
1456 GCGGG
HincII 3536 GTTAAC GT[CT]^[GA]AC
5613 GTTGAC
HindII 3536 GTTAAC GT[CT]^[GA]AC
5613 GTTGAC
MroI 1096 TCCGGA T^CCGGA
4771 TCCGGA
MspA1I 4439 CAGCTG C[AC]G^C[GT]G
4857 CAGCTG
MvnI 5078 CGCG CG^CG
5374 CGCG
NcoI 3061 CCATGG C^CATGG
5383 CCATGG
NsiI 4733 ATGCAT ATGCA^T
5692 ATGCAT
PvuII 4439 CAGCTG CAG^CTG
4857 CAGCTG
TaqI 4602 TCGA T^CGA
4612 TCGA
TthHB8I 4602 TCGA T^CGA
4612 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanII
BbsI BbvI BcnI BfaI BfrI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI SwaI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmnI