################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AgeI Aor51HI AscI Asp718I AviII AvrII BbeI BbrPI BcgI BglI BlnI BsaBI BsiEI BsiWI BsmBI BspDI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoNI FseI FspI HaeII KasI KpnI KspI MamI NaeI NarI NgoMIV NheI NotI NspV PaeR7I PinAI PmaCI PmeI PmlI PstI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SphI Sse8387I XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 3581 GTAGAC GT^[AC][GT]AC AclI 59 AACGTT AA^CGTT AcyI 134 GACGCC G[GA]^CG[CT]C AhdI 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1323 GTGCAC G^TGCAC AlwNI 86 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4463 CCTGAGG CC^T[ACGT]AGG ApaI 4419 GGGCCC GGGCC^C ApaLI 1323 GTGCAC G^TGCAC AspEI 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1509 CCCGGG C^[CT]CG[GA]G BbvCI 1533 GCTGAGG CCTCAGC(-5/-2) BciVI 3303 GTATCC GTATCC(6/5) BclI 892 TGATCA T^GATCA BglII 2500 AGATCT A^GATCT BlpI 4713 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4713 GCTCAGC GC^T[ACGT]AGC BsaAI 80 TACGTA [CT]AC^GT[GA] BsaHI 134 GACGCC G[GA]^CG[CT]C BsaI 2173 GAGACC GGTCTC(1/5) BsoBI 1509 CCCGGG C^[CT]CG[GA]G BsrDI 3432 GCAATG GCAATG(2/0) BssSI 1239 CTCGTG CACGAG(-5/-1) BstEII 714 GGTGACC G^GT[ACGT]ACC BstPI 714 GGTGACC G^GT[ACGT]ACC Bsu36I 4463 CCTGAGG CC^T[ACGT]AGG CelII 4713 GCTCAGC GC^T[ACGT]AGC Eam1105I 1693 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4463 CCTGAGG CC^T[ACGT]AGG EcoO65I 714 GGTGACC G^GT[ACGT]ACC EcoRV 1202 GATATC GAT^ATC FbaI 892 TGATCA T^GATCA Hin1I 134 GACGCC G[GA]^CG[CT]C HpaI 3536 GTTAAC GTT^AAC MfeI 329 CAATTG C^AATTG MluI 5077 ACGCGT A^CGCGT MunI 329 CAATTG C^AATTG NdeI 3469 CATATG CA^TATG NruI 5373 TCGCGA TCG^CGA PacI 1917 TTAATTAA TTAAT^TAA PflFI 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4254 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1576 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 59 AACGTT AA^CGTT ScaI 2762 AGTACT AGT^ACT SexAI 710 ACCTGGT A^CC[AT]GGT SmaI 1509 CCCGGG CCC^GGG SnaBI 80 TACGTA TAC^GTA SpeI 3130 ACTAGT A^CTAGT StuI 1864 AGGCCT AGG^CCT Tth111I 3018 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4254 CCATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 5108 TCTAGA T^CTAGA XmaCI 1509 CCCGGG C^CCGGG XmaI 1509 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 5078 CGCG CG^CG 5374 CGCG AccIII 1096 TCCGGA T^CCGGA 4771 TCCGGA BamHI 3255 GGATCC G^GATCC 5379 GGATCC BanI 2095 GGCACC G^G[CT][GA]CC 5516 GGCACC BpmI 1835 CTCCAG CTGGAG(16/14) 272 CTGGAG BsaWI 1096 TCCGGA [AT]^CCGG[AT] 4771 TCCGGA BseAI 1096 TCCGGA T^CCGGA 4771 TCCGGA BsgI 139 CTGCAC GTGCAG(16/14) 4374 CTGCAC BspEI 1096 TCCGGA T^CCGGA 4771 TCCGGA BstUI 5078 CGCG CG^CG 5374 CGCG DrdI 3718 GACCTTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4259 GACTGGGTAGTC EcoT22I 4733 ATGCAT ATGCA^T 5692 ATGCAT FauI 1358 GCGGG CCCGC(4/6) 1456 GCGGG HincII 3536 GTTAAC GT[CT]^[GA]AC 5613 GTTGAC HindII 3536 GTTAAC GT[CT]^[GA]AC 5613 GTTGAC MroI 1096 TCCGGA T^CCGGA 4771 TCCGGA MspA1I 4439 CAGCTG C[AC]G^C[GT]G 4857 CAGCTG MvnI 5078 CGCG CG^CG 5374 CGCG NcoI 3061 CCATGG C^CATGG 5383 CCATGG NsiI 4733 ATGCAT ATGCA^T 5692 ATGCAT PvuII 4439 CAGCTG CAG^CTG 4857 CAGCTG TaqI 4602 TCGA T^CGA 4612 TCGA TthHB8I 4602 TCGA T^CGA 4612 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI SwaI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmnI