################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccI AccIII AclI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI AviII AvrII BamHI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI Eam1105I EcoRV FseI FspI HpaI MamI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SmaI SpeI Sse8387I SspBI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1820 GGTACC G^GTACC AflII 2169 CTTAAG C^TTAAG Aor51HI 465 AGCGCT AGC^GCT Asp700I 1662 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1820 GGTACC G^GTACC AspI 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3369 GGCGCC GGCGC^C BbrPI 4449 CACGTG CAC^GTG BclI 247 TGATCA T^GATCA BfrI 2169 CTTAAG C^TTAAG BglI 162 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4277 AGATCT A^GATCT BsiEI 4719 CGGCCG CG[GA][CT]^CG BsmI 2210 GCATTC GAATGC(1/-1) BsrBI 178 GAGCGG CCGCTC(-3/-3) BssHII 105 GCGCGC G^CGCGC BssSI 5182 CACGAG CACGAG(-5/-1) DrdI 1041 GACATTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4719 CGGCCG C^GGCCG EclXI 4719 CGGCCG C^GGCCG Eco47III 465 AGCGCT AGC^GCT Eco52I 4719 CGGCCG C^GGCCG EcoT22I 265 ATGCAT ATGCA^T FbaI 247 TGATCA T^GATCA HincII 4509 GTTGAC GT[CT]^[GA]AC HindII 4509 GTTGAC GT[CT]^[GA]AC KasI 3369 GGCGCC G^GCGCC KpnI 1820 GGTACC GGTAC^C NarI 3369 GGCGCC GG^CGCC NdeI 5012 CATATG CA^TATG NheI 2145 GCTAGC G^CTAGC NsiI 265 ATGCAT ATGCA^T PaeR7I 461 CTCGAG C^TCGAG PflFI 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4449 CACGTG CAC^GTG PmlI 4449 CACGTG CAC^GTG PstI 2443 CTGCAG CTGCA^G SapI 4371 GAAGAGC GCTCTTC(1/4) ScaI 1553 AGTACT AGT^ACT SfoI 3369 GGCGCC GGC^GCC SgrAI 4605 CACCGGTG C[GA]^CCGG[CT]G SnaBI 2705 TACGTA TAC^GTA SphI 4922 GCATGC GCATG^C SspI 2055 AATATT AAT^ATT Tth111I 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 196 CCATCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 461 CTCGAG C^TCGAG XmnI 1662 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 1962 GACGCC G[GA]^CG[CT]C 3369 GGCGCC AgeI 335 ACCGGT A^CCGGT 4606 ACCGGT BalI 3633 TGGCCA TGG^CCA 4166 TGGCCA BcgI 2585 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2950 GCAGCGGGTTCG BciVI 356 GGATAC GTATCC(6/5) 924 GTATCC BlpI 1427 GCTCAGC GC^T[ACGT]AGC 2969 GCTGAGC Bpu1102I 1427 GCTCAGC GC^T[ACGT]AGC 2969 GCTGAGC BsaHI 1962 GACGCC G[GA]^CG[CT]C 3369 GGCGCC BspMI 722 GCAGGT ACCTGC(4/8) 2650 GCAGGT BsrDI 3106 GCAATG GCAATG(2/0) 3280 GCAATG BstEII 4017 GGTGACC G^GT[ACGT]ACC 4529 GGTGACC BstPI 4017 GGTGACC G^GT[ACGT]ACC 4529 GGTGACC BstYI 2338 GGATCT [GA]^GATC[CT] 4277 AGATCT CelII 1427 GCTCAGC GC^T[ACGT]AGC 2969 GCTGAGC DraII 2995 AGGACCC [GA]G^G[ACGT]CC[CT] 4623 AGGTCCT EcoO109I 2995 AGGACCC [GA]G^G[ACGT]CC[CT] 4623 AGGTCCT EcoO65I 4017 GGTGACC G^GT[ACGT]ACC 4529 GGTGACC EcoRI 1339 GAATTC G^AATTC 3860 GAATTC Hin1I 1962 GACGCC G[GA]^CG[CT]C 3369 GGCGCC HindIII 3306 AAGCTT A^AGCTT 5111 AAGCTT KspI 42 CCGCGG CCGC^GG 166 CCGCGG MflI 2338 GGATCT [GA]^GATC[CT] 4277 AGATCT MluNI 3633 TGGCCA TGG^CCA 4166 TGGCCA MscI 3633 TGGCCA TGG^CCA 4166 TGGCCA NcoI 4313 CCATGG C^CATGG 4992 CCATGG PinAI 335 ACCGGT A^CCGGT 4606 ACCGGT PpuMI 2995 AGGACCC [GA]G^G[AT]CC[CT] 4623 AGGTCCT SacII 42 CCGCGG CCGC^GG 166 CCGCGG XhoII 2338 GGATCT [GA]^GATC[CT] 4277 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraIII DsaI EaeI EarI Eco81I EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I