##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AccIII AclI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BamHI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
Eam1105I EcoRV FseI FspI HpaI
MamI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfuI SmaI
SpeI Sse8387I SspBI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1820 GGTACC G^GTACC
AflII 2169 CTTAAG C^TTAAG
Aor51HI 465 AGCGCT AGC^GCT
Asp700I 1662 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1820 GGTACC G^GTACC
AspI 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3369 GGCGCC GGCGC^C
BbrPI 4449 CACGTG CAC^GTG
BclI 247 TGATCA T^GATCA
BfrI 2169 CTTAAG C^TTAAG
BglI 162 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4277 AGATCT A^GATCT
BsiEI 4719 CGGCCG CG[GA][CT]^CG
BsmI 2210 GCATTC GAATGC(1/-1)
BsrBI 178 GAGCGG CCGCTC(-3/-3)
BssHII 105 GCGCGC G^CGCGC
BssSI 5182 CACGAG CACGAG(-5/-1)
DrdI 1041 GACATTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4719 CGGCCG C^GGCCG
EclXI 4719 CGGCCG C^GGCCG
Eco47III 465 AGCGCT AGC^GCT
Eco52I 4719 CGGCCG C^GGCCG
EcoT22I 265 ATGCAT ATGCA^T
FbaI 247 TGATCA T^GATCA
HincII 4509 GTTGAC GT[CT]^[GA]AC
HindII 4509 GTTGAC GT[CT]^[GA]AC
KasI 3369 GGCGCC G^GCGCC
KpnI 1820 GGTACC GGTAC^C
NarI 3369 GGCGCC GG^CGCC
NdeI 5012 CATATG CA^TATG
NheI 2145 GCTAGC G^CTAGC
NsiI 265 ATGCAT ATGCA^T
PaeR7I 461 CTCGAG C^TCGAG
PflFI 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4449 CACGTG CAC^GTG
PmlI 4449 CACGTG CAC^GTG
PstI 2443 CTGCAG CTGCA^G
SapI 4371 GAAGAGC GCTCTTC(1/4)
ScaI 1553 AGTACT AGT^ACT
SfoI 3369 GGCGCC GGC^GCC
SgrAI 4605 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 2705 TACGTA TAC^GTA
SphI 4922 GCATGC GCATG^C
SspI 2055 AATATT AAT^ATT
Tth111I 3247 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 196 CCATCAAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 461 CTCGAG C^TCGAG
XmnI 1662 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1962 GACGCC G[GA]^CG[CT]C
3369 GGCGCC
AgeI 335 ACCGGT A^CCGGT
4606 ACCGGT
BalI 3633 TGGCCA TGG^CCA
4166 TGGCCA
BcgI 2585 GCAGCTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2950 GCAGCGGGTTCG
BciVI 356 GGATAC GTATCC(6/5)
924 GTATCC
BlpI 1427 GCTCAGC GC^T[ACGT]AGC
2969 GCTGAGC
Bpu1102I 1427 GCTCAGC GC^T[ACGT]AGC
2969 GCTGAGC
BsaHI 1962 GACGCC G[GA]^CG[CT]C
3369 GGCGCC
BspMI 722 GCAGGT ACCTGC(4/8)
2650 GCAGGT
BsrDI 3106 GCAATG GCAATG(2/0)
3280 GCAATG
BstEII 4017 GGTGACC G^GT[ACGT]ACC
4529 GGTGACC
BstPI 4017 GGTGACC G^GT[ACGT]ACC
4529 GGTGACC
BstYI 2338 GGATCT [GA]^GATC[CT]
4277 AGATCT
CelII 1427 GCTCAGC GC^T[ACGT]AGC
2969 GCTGAGC
DraII 2995 AGGACCC [GA]G^G[ACGT]CC[CT]
4623 AGGTCCT
EcoO109I 2995 AGGACCC [GA]G^G[ACGT]CC[CT]
4623 AGGTCCT
EcoO65I 4017 GGTGACC G^GT[ACGT]ACC
4529 GGTGACC
EcoRI 1339 GAATTC G^AATTC
3860 GAATTC
Hin1I 1962 GACGCC G[GA]^CG[CT]C
3369 GGCGCC
HindIII 3306 AAGCTT A^AGCTT
5111 AAGCTT
KspI 42 CCGCGG CCGC^GG
166 CCGCGG
MflI 2338 GGATCT [GA]^GATC[CT]
4277 AGATCT
MluNI 3633 TGGCCA TGG^CCA
4166 TGGCCA
MscI 3633 TGGCCA TGG^CCA
4166 TGGCCA
NcoI 4313 CCATGG C^CATGG
4992 CCATGG
PinAI 335 ACCGGT A^CCGGT
4606 ACCGGT
PpuMI 2995 AGGACCC [GA]G^G[AT]CC[CT]
4623 AGGTCCT
SacII 42 CCGCGG CCGC^GG
166 CCGCGG
XhoII 2338 GGATCT [GA]^GATC[CT]
4277 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraIII DsaI
EaeI EarI Eco81I EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I