##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII BbeI
BbrPI BcgI BglI BsiEI BsiWI
BsmBI BspDI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII KasI KpnI KspI MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SpeI Sse8387I StuI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1023 GACGTC GACGT^C
AccIII 2068 TCCGGA T^CCGGA
AciI 1573 CCGC CCGC(-3/-1)
AcyI 1023 GACGTC G[GA]^CG[CT]C
AflII 4410 CTTAAG C^TTAAG
AflIII 3667 ACGTGT A^C[GA][CT]GT
AocI 2162 CCTAAGG CC^T[ACGT]AGG
AvrII 1013 CCTAGG C^CTAGG
BalI 2006 TGGCCA TGG^CCA
BamHI 2145 GGATCC G^GATCC
BbvCI 3154 CCTCAGC CCTCAGC(-5/-2)
BciVI 906 GGATAC GTATCC(6/5)
BclI 4387 TGATCA T^GATCA
BfrI 4410 CTTAAG C^TTAAG
BglII 2420 AGATCT A^GATCT
BlnI 1013 CCTAGG C^CTAGG
BlpI 1598 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1598 GCTTAGC GC^T[ACGT]AGC
BsaAI 4045 TACGTA [CT]AC^GT[GA]
BsaHI 1023 GACGTC G[GA]^CG[CT]C
BsaI 5034 GGTCTC GGTCTC(1/5)
BsaWI 2068 TCCGGA [AT]^CCGG[AT]
BseAI 2068 TCCGGA T^CCGGA
Bsp1407I 2284 TGTACA T^GTACA
BspEI 2068 TCCGGA T^CCGGA
BspHI 4798 TCATGA T^CATGA
BsrGI 2284 TGTACA T^GTACA
BstBI 3854 TTCGAA TT^CGAA
Bsu36I 2162 CCTAAGG CC^T[ACGT]AGG
CelII 1598 GCTTAGC GC^T[ACGT]AGC
DraIII 3007 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3833 GACGGTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2006 TGGCCA [CT]^GGCC[GA]
Eco81I 2162 CCTAAGG CC^T[ACGT]AGG
EcoT22I 799 ATGCAT ATGCA^T
FbaI 4387 TGATCA T^GATCA
HgaI 563 GCGTC GACGC(5/10)
Hin1I 1023 GACGTC G[GA]^CG[CT]C
MfeI 4886 CAATTG C^AATTG
MluNI 2006 TGGCCA TGG^CCA
MroI 2068 TCCGGA T^CCGGA
MscI 2006 TGGCCA TGG^CCA
MspA1I 4104 CAGCTG C[AC]G^C[GT]G
MunI 4886 CAATTG C^AATTG
NsiI 799 ATGCAT ATGCA^T
NspV 3854 TTCGAA TT^CGAA
PvuII 4104 CAGCTG CAG^CTG
RcaI 4798 TCATGA T^CATGA
SacI 879 GAGCTC GAGCT^C
ScaI 1392 AGTACT AGT^ACT
SfuI 3854 TTCGAA TT^CGAA
SnaBI 4045 TACGTA TAC^GTA
SphI 994 GCATGC GCATG^C
SspBI 2284 TGTACA T^GTACA
SwaI 229 ATTTAAAT ATTT^AAAT
XcmI 1408 CCACGAAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AseI 1046 ATTAAT AT^TAAT
4134 ATTAAT
AsnI 1046 ATTAAT AT^TAAT
4134 ATTAAT
AvaI 1675 CTCGGG C^[CT]CG[GA]G
2517 CCCGAG
BcnI 3293 CCGGG CC^[GC]GG
3771 CCGGG
BsaBI 3111 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4754 GATATACATC
BseRI 589 CTCCTC GAGGAG(10/8)
5112 GAGGAG
BsoBI 1675 CTCGGG C^[CT]CG[GA]G
2517 CCCGAG
BspMI 2771 ACCTGC ACCTGC(4/8)
5002 ACCTGC
CfoI 3078 GCGC GCG^C
3122 GCGC
EcoNI 766 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2003 CCTTGGCCAGG
EcoRV 1776 GATATC GAT^ATC
3531 GATATC
HhaI 3078 GCGC GCG^C
3122 GCGC
HinP1I 3078 GCGC G^CGC
3122 GCGC
MamI 3111 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4754 GATATACATC
NciI 3293 CCGGG CC^[GC]GG
3771 CCGGG
PshBI 1046 ATTAAT AT^TAAT
4134 ATTAAT
Tsp45I 813 GTCAC ^GT[GC]AC
3013 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I XhoII XmnI