##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Aor51HI ApaI AscI
AspEI AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BcgI BclI
BcnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRV FauI FbaI
FseI FspI HaeII HgaI Hin1I
KasI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1617 GGTACC G^GTACC
AccI 2183 GTCGAC GT^[AC][GT]AC
AccII 5544 CGCG CG^CG
Alw44I 5686 GTGCAC G^TGCAC
AocI 2970 CCTCAGG CC^T[ACGT]AGG
ApaLI 5686 GTGCAC G^TGCAC
Asp718I 1617 GGTACC G^GTACC
AvrII 1608 CCTAGG C^CTAGG
BalI 5627 TGGCCA TGG^CCA
BanI 1617 GGTACC G^G[CT][GA]CC
BanII 2518 GGGCTC G[GA]GC[CT]^C
BglI 5629 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1608 CCTAGG C^CTAGG
BpmI 5779 CTCCAG CTGGAG(16/14)
BsaAI 2680 CACGTA [CT]AC^GT[GA]
BseRI 706 GAGGAG GAGGAG(10/8)
BssSI 5718 CACGAG CACGAG(-5/-1)
BstAPI 282 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3981 GGTCACC G^GT[ACGT]ACC
BstPI 3981 GGTCACC G^GT[ACGT]ACC
BstUI 5544 CGCG CG^CG
Bsu36I 2970 CCTCAGG CC^T[ACGT]AGG
CfoI 5543 GCGC GCG^C
DraIII 2451 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 44 GACCTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 5627 TGGCCA [CT]^GGCC[GA]
Eco81I 2970 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3981 GGTCACC G^GT[ACGT]ACC
HapII 2373 CCGG C^CGG
HhaI 5543 GCGC GCG^C
HinP1I 5543 GCGC G^CGC
HpaI 4489 GTTAAC GTT^AAC
HpaII 2373 CCGG C^CGG
KpnI 1617 GGTACC GGTAC^C
MluNI 5627 TGGCCA TGG^CCA
MscI 5627 TGGCCA TGG^CCA
MspA1I 2852 CCGCTG C[AC]G^C[GT]G
MspI 2373 CCGG C^CGG
MvnI 5544 CGCG CG^CG
PpuMI 2263 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1222 CTGCAG CTGCA^G
SalI 2183 GTCGAC G^TCGAC
XbaI 401 TCTAGA T^CTAGA
XcmI 789 CCACCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflII 628 CTTAAG C^TTAAG
4432 CTTAAG
AlwNI 2152 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2600 CAGGGCCTG
AseI 1645 ATTAAT AT^TAAT
3007 ATTAAT
AsnI 1645 ATTAAT AT^TAAT
3007 ATTAAT
AvaII 1217 GGACC G^G[AT]CC
2264 GGTCC
BfrI 628 CTTAAG C^TTAAG
4432 CTTAAG
BspMI 1112 ACCTGC ACCTGC(4/8)
4001 GCAGGT
EcoNI 491 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1608 CCTAGGCAAGG
EcoT22I 97 ATGCAT ATGCA^T
1130 ATGCAT
HincII 2183 GTCGAC GT[CT]^[GA]AC
4489 GTTAAC
HindII 2183 GTCGAC GT[CT]^[GA]AC
4489 GTTAAC
NdeI 4499 CATATG CA^TATG
5382 CATATG
NsiI 97 ATGCAT ATGCA^T
1130 ATGCAT
PshBI 1645 ATTAAT AT^TAAT
3007 ATTAAT
SphI 2466 GCATGC GCATG^C
2950 GCATGC
StuI 1605 AGGCCT AGG^CCT
4778 AGGCCT
SwaI 1773 ATTTAAAT ATTT^AAAT
4404 ATTTAAAT
TaqI 930 TCGA T^CGA
2184 TCGA
TthHB8I 930 TCGA T^CGA
2184 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BbsI BbvI
BciVI BfaI BglII BmyI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmnI