################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI Aor51HI ApaI AscI AspEI AspI AvaI AviII BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRV FauI FbaI FseI FspI HaeII HgaI Hin1I KasI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1617 GGTACC G^GTACC AccI 2183 GTCGAC GT^[AC][GT]AC AccII 5544 CGCG CG^CG Alw44I 5686 GTGCAC G^TGCAC AocI 2970 CCTCAGG CC^T[ACGT]AGG ApaLI 5686 GTGCAC G^TGCAC Asp718I 1617 GGTACC G^GTACC AvrII 1608 CCTAGG C^CTAGG BalI 5627 TGGCCA TGG^CCA BanI 1617 GGTACC G^G[CT][GA]CC BanII 2518 GGGCTC G[GA]GC[CT]^C BglI 5629 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1608 CCTAGG C^CTAGG BpmI 5779 CTCCAG CTGGAG(16/14) BsaAI 2680 CACGTA [CT]AC^GT[GA] BseRI 706 GAGGAG GAGGAG(10/8) BssSI 5718 CACGAG CACGAG(-5/-1) BstAPI 282 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3981 GGTCACC G^GT[ACGT]ACC BstPI 3981 GGTCACC G^GT[ACGT]ACC BstUI 5544 CGCG CG^CG Bsu36I 2970 CCTCAGG CC^T[ACGT]AGG CfoI 5543 GCGC GCG^C DraIII 2451 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 44 GACCTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 5627 TGGCCA [CT]^GGCC[GA] Eco81I 2970 CCTCAGG CC^T[ACGT]AGG EcoO65I 3981 GGTCACC G^GT[ACGT]ACC HapII 2373 CCGG C^CGG HhaI 5543 GCGC GCG^C HinP1I 5543 GCGC G^CGC HpaI 4489 GTTAAC GTT^AAC HpaII 2373 CCGG C^CGG KpnI 1617 GGTACC GGTAC^C MluNI 5627 TGGCCA TGG^CCA MscI 5627 TGGCCA TGG^CCA MspA1I 2852 CCGCTG C[AC]G^C[GT]G MspI 2373 CCGG C^CGG MvnI 5544 CGCG CG^CG PpuMI 2263 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1222 CTGCAG CTGCA^G SalI 2183 GTCGAC G^TCGAC XbaI 401 TCTAGA T^CTAGA XcmI 789 CCACCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflII 628 CTTAAG C^TTAAG 4432 CTTAAG AlwNI 2152 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2600 CAGGGCCTG AseI 1645 ATTAAT AT^TAAT 3007 ATTAAT AsnI 1645 ATTAAT AT^TAAT 3007 ATTAAT AvaII 1217 GGACC G^G[AT]CC 2264 GGTCC BfrI 628 CTTAAG C^TTAAG 4432 CTTAAG BspMI 1112 ACCTGC ACCTGC(4/8) 4001 GCAGGT EcoNI 491 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1608 CCTAGGCAAGG EcoT22I 97 ATGCAT ATGCA^T 1130 ATGCAT HincII 2183 GTCGAC GT[CT]^[GA]AC 4489 GTTAAC HindII 2183 GTCGAC GT[CT]^[GA]AC 4489 GTTAAC NdeI 4499 CATATG CA^TATG 5382 CATATG NsiI 97 ATGCAT ATGCA^T 1130 ATGCAT PshBI 1645 ATTAAT AT^TAAT 3007 ATTAAT SphI 2466 GCATGC GCATG^C 2950 GCATGC StuI 1605 AGGCCT AGG^CCT 4778 AGGCCT SwaI 1773 ATTTAAAT ATTT^AAAT 4404 ATTTAAAT TaqI 930 TCGA T^CGA 2184 TCGA TthHB8I 930 TCGA T^CGA 2184 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI BbsI BbvI BciVI BfaI BglII BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmnI