##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AclI AocI Aor51HI ApaI
AscI AviII AvrII BbrPI BglI
BlnI BsaAI BsaBI BsiWI Bsp1407I
BspLU11I BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
CpoI Eco47III Eco81I EcoNI EcoO65I
FseI FspI HindIII KspI MamI
NaeI NgoMIV NotI NruI PaeR7I
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 4640 GGTACC G^GTACC
AccII 3158 CGCG CG^CG
AccIII 1177 TCCGGA T^CCGGA
AflII 195 CTTAAG C^TTAAG
AflIII 3157 ACGCGT A^C[GA][CT]GT
AgeI 4102 ACCGGT A^CCGGT
Alw44I 3302 GTGCAC G^TGCAC
ApaLI 3302 GTGCAC G^TGCAC
AseI 5344 ATTAAT AT^TAAT
AsnI 5344 ATTAAT AT^TAAT
Asp718I 4640 GGTACC G^GTACC
BalI 2264 TGGCCA TGG^CCA
BamHI 3142 GGATCC G^GATCC
BbeI 3904 GGCGCC GGCGC^C
BfrI 195 CTTAAG C^TTAAG
BglII 4212 AGATCT A^GATCT
BlpI 4496 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4496 GCTGAGC GC^T[ACGT]AGC
BseAI 1177 TCCGGA T^CCGGA
BspDI 1131 ATCGAT AT^CGAT
BspEI 1177 TCCGGA T^CCGGA
BspHI 1507 TCATGA T^CATGA
BsrBI 555 GAGCGG CCGCTC(-3/-3)
BstBI 1330 TTCGAA TT^CGAA
BstUI 3158 CGCG CG^CG
BstXI 2846 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4496 GCTGAGC GC^T[ACGT]AGC
ClaI 1131 ATCGAT AT^CGAT
DraII 3647 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 3647 GGGTCCT [GA]G^G[ACGT]CC[CT]
HpaI 1157 GTTAAC GTT^AAC
KasI 3904 GGCGCC G^GCGCC
KpnI 4640 GGTACC GGTAC^C
MfeI 5568 CAATTG C^AATTG
MluI 3157 ACGCGT A^CGCGT
MluNI 2264 TGGCCA TGG^CCA
MroI 1177 TCCGGA T^CCGGA
MscI 2264 TGGCCA TGG^CCA
MunI 5568 CAATTG C^AATTG
MvnI 3158 CGCG CG^CG
NarI 3904 GGCGCC GG^CGCC
NcoI 5465 CCATGG C^CATGG
NdeI 3741 CATATG CA^TATG
NheI 620 GCTAGC G^CTAGC
NspV 1330 TTCGAA TT^CGAA
PacI 5249 TTAATTAA TTAAT^TAA
PinAI 4102 ACCGGT A^CCGGT
PpuMI 3647 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 5344 ATTAAT AT^TAAT
PstI 3209 CTGCAG CTGCA^G
RcaI 1507 TCATGA T^CATGA
SacI 4849 GAGCTC GAGCT^C
SalI 4327 GTCGAC G^TCGAC
SapI 3402 GCTCTTC GCTCTTC(1/4)
SfoI 3904 GGCGCC GGC^GCC
SfuI 1330 TTCGAA TT^CGAA
SmlI 195 CTTAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1000 GTAGAC GT^[AC][GT]AC
4327 GTCGAC
AcyI 2571 GGCGTC G[GA]^CG[CT]C
3904 GGCGCC
AhdI 2717 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3999 GACCCCTTGTC
AspEI 2717 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3999 GACCCCTTGTC
AspI 1371 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3226 GACTCTGTC
BanI 3904 GGCGCC G^G[CT][GA]CC
4640 GGTACC
BbvCI 1121 CCTCAGC CCTCAGC(-5/-2)
2363 CCTCAGC
BciVI 4052 GGATAC GTATCC(6/5)
4647 GGATAC
BclI 3613 TGATCA T^GATCA
3938 TGATCA
BsaHI 2571 GGCGTC G[GA]^CG[CT]C
3904 GGCGCC
BsaI 1656 GAGACC GGTCTC(1/5)
4272 GAGACC
BsiEI 1026 CGGCCG CG[GA][CT]^CG
4147 CGGCCG
BsmBI 1013 CGTCTC CGTCTC(1/5)
4475 GAGACG
DraIII 1296 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2766 CACACTGTG
DrdI 1208 GACCTTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3042 GACAGAACGGTC
DsaI 1061 CCACGG C^C[GA][CT]GG
5465 CCATGG
EagI 1026 CGGCCG C^GGCCG
4147 CGGCCG
Eam1105I 2717 GACAATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3999 GACCCCTTGTC
EclXI 1026 CGGCCG C^GGCCG
4147 CGGCCG
Eco52I 1026 CGGCCG C^GGCCG
4147 CGGCCG
EcoRI 768 GAATTC G^AATTC
1568 GAATTC
EcoRV 2988 GATATC GAT^ATC
4871 GATATC
EcoT22I 3674 ATGCAT ATGCA^T
4691 ATGCAT
FauI 2425 CCCGC CCCGC(4/6)
3084 GCGGG
FbaI 3613 TGATCA T^GATCA
3938 TGATCA
HgaI 1104 GACGC GACGC(5/10)
2572 GCGTC
Hin1I 2571 GGCGTC G[GA]^CG[CT]C
3904 GGCGCC
NsiI 3674 ATGCAT ATGCA^T
4691 ATGCAT
PflFI 1371 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3226 GACTCTGTC
PflMI 2246 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4572 CCAGAGACTGG
SmaI 49 CCCGGG CCC^GGG
2441 CCCGGG
Tth111I 1371 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3226 GACTCTGTC
Van91I 2246 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4572 CCAGAGACTGG
XbaI 798 TCTAGA T^CTAGA
3287 TCTAGA
XcmI 116 CCAAGGTTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4430 CCATAACACTAATGG
XmaCI 49 CCCGGG C^CCGGG
2441 CCCGGG
XmaI 49 CCCGGG C^CCGGG
2441 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI