##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BamHI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRV FseI HpaI
KspI MfeI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2245 GTCTAC GT^[AC][GT]AC
AflII 4541 CTTAAG C^TTAAG
BciVI 1276 GTATCC GTATCC(6/5)
BfrI 4541 CTTAAG C^TTAAG
BsaBI 4337 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4109 ACCGGA [AT]^CCGG[AT]
BspHI 3078 TCATGA T^CATGA
BsrDI 258 GCAATG GCAATG(2/0)
BstEII 2598 GGTGACC G^GT[ACGT]ACC
BstPI 2598 GGTGACC G^GT[ACGT]ACC
DrdI 1333 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2598 GGTGACC G^GT[ACGT]ACC
HindIII 3219 AAGCTT A^AGCTT
MamI 4337 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1602 ACGCGT A^CGCGT
PaeR7I 2209 CTCGAG C^TCGAG
PshAI 4685 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3078 TCATGA T^CATGA
SapI 3319 GCTCTTC GCTCTTC(1/4)
SfiI 880 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XhoI 2209 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 995 GGTACC G^GTACC
2682 GGTACC
AflIII 1602 ACGCGT A^C[GA][CT]GT
5095 ACGTGT
Asp700I 2713 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4653 GAATTACTTC
Asp718I 995 GGTACC G^GTACC
2682 GGTACC
BbrPI 949 CACGTG CAC^GTG
1570 CACGTG
BclI 1613 TGATCA T^GATCA
4942 TGATCA
BlpI 2779 GCTGAGC GC^T[ACGT]AGC
4378 GCTCAGC
Bpu1102I 2779 GCTGAGC GC^T[ACGT]AGC
4378 GCTCAGC
BsmI 5230 GAATGC GAATGC(1/-1)
2667 GCATTC
BsrBI 3882 CCGCTC CCGCTC(-3/-3)
3708 GAGCGG
BsrFI 440 ACCGGC [GA]^CCGG[CT]
2384 ACCGGC
BssSI 565 CACGAG CACGAG(-5/-1)
723 CTCGTG
CelII 2779 GCTGAGC GC^T[ACGT]AGC
4378 GCTCAGC
Cfr10I 440 ACCGGC [GA]^CCGG[CT]
2384 ACCGGC
DraI 4145 TTTAAA TTT^AAA
5310 TTTAAA
EagI 488 CGGCCG C^GGCCG
872 CGGCCG
EclXI 488 CGGCCG C^GGCCG
872 CGGCCG
Eco52I 488 CGGCCG C^GGCCG
872 CGGCCG
EcoT22I 3185 ATGCAT ATGCA^T
4140 ATGCAT
FbaI 1613 TGATCA T^GATCA
4942 TGATCA
KpnI 995 GGTACC GGTAC^C
2682 GGTACC
NsiI 3185 ATGCAT ATGCA^T
4140 ATGCAT
PmaCI 949 CACGTG CAC^GTG
1570 CACGTG
PmlI 949 CACGTG CAC^GTG
1570 CACGTG
SbfI 1230 CCTGCAGG CCTGCA^GG
2466 CCTGCAGG
SphI 338 GCATGC GCATG^C
3526 GCATGC
Sse8387I 1230 CCTGCAGG CCTGCA^GG
2466 CCTGCAGG
XmnI 2713 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4653 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII AviII AvrII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI