##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AscI AspI AviII BbeI
BbrPI BfrI BsaWI BseAI BspDI
BspEI BspLU11I BssHII BstUI ClaI
CpoI EagI EclXI Eco52I EcoT22I
FseI FspI KasI KspI MluI
MroI MvnI NarI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 543 ACGTGT A^C[GA][CT]GT
Alw44I 5322 GTGCAC G^TGCAC
ApaI 5755 GGGCCC GGGCC^C
ApaLI 5322 GTGCAC G^TGCAC
AseI 874 ATTAAT AT^TAAT
AsnI 874 ATTAAT AT^TAAT
AvrII 2238 CCTAGG C^CTAGG
BciVI 5484 GGATAC GTATCC(6/5)
BclI 2973 TGATCA T^GATCA
BglI 5757 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2238 CCTAGG C^CTAGG
BsaBI 4352 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 605 CGACCG CG[GA][CT]^CG
BsiWI 1326 CGTACG C^GTACG
BsmBI 2954 CGTCTC CGTCTC(1/5)
BssSI 1295 CTCGTG CACGAG(-5/-1)
Bst1107I 4615 GTATAC GTA^TAC
BstAPI 5257 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1833 GGTGACC G^GT[ACGT]ACC
BstPI 1833 GGTGACC G^GT[ACGT]ACC
BstZ17I 4615 GTATAC GTA^TAC
DrdI 1155 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1833 GGTGACC G^GT[ACGT]ACC
EcoRV 2593 GATATC GAT^ATC
FbaI 2973 TGATCA T^GATCA
HindIII 4034 AAGCTT A^AGCTT
HpaI 2103 GTTAAC GTT^AAC
MamI 4352 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 11 CAATTG C^AATTG
MunI 11 CAATTG C^AATTG
NdeI 728 CATATG CA^TATG
PflMI 772 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 874 ATTAAT AT^TAAT
SacI 5275 GAGCTC GAGCT^C
SapI 3084 GAAGAGC GCTCTTC(1/4)
SmaI 2847 CCCGGG CCC^GGG
Van91I 772 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2847 CCCGGG C^CCGGG
XmaI 2847 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 5135 GGTACC G^GTACC
5477 GGTACC
AcyI 2344 GGCGTC G[GA]^CG[CT]C
5778 GGCGTC
Aor51HI 1460 AGCGCT AGC^GCT
4659 AGCGCT
Asp718I 5135 GGTACC G^GTACC
5477 GGTACC
AspHI 5275 GAGCTC G[AT]GC[AT]^C
5322 GTGCAC
BglII 2490 AGATCT A^GATCT
4106 AGATCT
BsaHI 2344 GGCGTC G[GA]^CG[CT]C
5778 GGCGTC
BsiHKAI 5275 GAGCTC G[AT]GC[AT]^C
5322 GTGCAC
BsmI 1758 GAATGC GAATGC(1/-1)
2034 GAATGC
BsrFI 2225 GCCGGC [GA]^CCGG[CT]
3230 GCCGGC
Cfr10I 2225 GCCGGC [GA]^CCGG[CT]
3230 GCCGGC
Eco47III 1460 AGCGCT AGC^GCT
4659 AGCGCT
EcoNI 3397 CCTTCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5745 CCTGTGGGAGG
Hin1I 2344 GGCGTC G[GA]^CG[CT]C
5778 GGCGTC
KpnI 5135 GGTACC GGTAC^C
5477 GGTACC
NaeI 2225 GCCGGC GCC^GGC
3230 GCCGGC
NgoMIV 2225 GCCGGC G^CCGGC
3230 GCCGGC
PshAI 2162 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5774 GACTGGCGTC
SspI 4761 AATATT AAT^ATT
4976 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwI AlwNI AocI ApoI
Asp700I AspEI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstBI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI NspV
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfuI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI