##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BcgI BciVI BfrI BlnI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI BstBI ClaI CpoI EcoRI
EcoRV FseI FspI HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NspV
PacI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfoI SfuI Sse8387I SspBI
SwaI Van91I
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 276 GACGTC GACGT^C
AccIII 4047 TCCGGA T^CCGGA
AhdI 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3841 GTGCAC G^TGCAC
Aor51HI 3786 AGCGCT AGC^GCT
ApaLI 3841 GTGCAC G^TGCAC
AspEI 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3956 CACGTG CAC^GTG
BseAI 4047 TCCGGA T^CCGGA
BsiEI 4354 CGGCCG CG[GA][CT]^CG
BspEI 4047 TCCGGA T^CCGGA
BspHI 990 TCATGA T^CATGA
BsrDI 4701 GCAATG GCAATG(2/0)
Bst1107I 4330 GTATAC GTA^TAC
BstEII 1211 GGTCACC G^GT[ACGT]ACC
BstPI 1211 GGTCACC G^GT[ACGT]ACC
BstZ17I 4330 GTATAC GTA^TAC
DrdI 693 GACTCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4354 CGGCCG C^GGCCG
Eam1105I 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4354 CGGCCG C^GGCCG
Eco47III 3786 AGCGCT AGC^GCT
Eco52I 4354 CGGCCG C^GGCCG
EcoO65I 1211 GGTCACC G^GT[ACGT]ACC
EcoT22I 5043 ATGCAT ATGCA^T
MroI 4047 TCCGGA T^CCGGA
NaeI 1911 GCCGGC GCC^GGC
NgoMIV 1911 GCCGGC G^CCGGC
NsiI 5043 ATGCAT ATGCA^T
PmaCI 3956 CACGTG CAC^GTG
PmlI 3956 CACGTG CAC^GTG
RcaI 990 TCATGA T^CATGA
SacI 4033 GAGCTC GAGCT^C
ScaI 5005 AGTACT AGT^ACT
SexAI 4025 ACCAGGT A^CC[AT]GGT
SfiI 1 GGCCGTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1910 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3969 CCCGGG CCC^GGG
SnaBI 165 TACGTA TAC^GTA
SpeI 4818 ACTAGT A^CTAGT
SspI 5110 AATATT AAT^ATT
XbaI 280 TCTAGA T^CTAGA
XmaCI 3969 CCCGGG C^CCGGG
XmaI 3969 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1832 GTCTAC GT^[AC][GT]AC
4330 GTATAC
ApaI 481 GGGCCC GGGCC^C
4371 GGGCCC
BamHI 1429 GGATCC G^GATCC
2509 GGATCC
BbvCI 3715 CCTCAGC CCTCAGC(-5/-2)
4782 CCTCAGC
BglI 2 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2651 GCCCGTCTGGC
BlpI 401 GCTAAGC GC^T[ACGT]AGC
3982 GCTGAGC
Bpu1102I 401 GCTAAGC GC^T[ACGT]AGC
3982 GCTGAGC
BsaAI 165 TACGTA [CT]AC^GT[GA]
3956 CACGTG
BsaBI 1477 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATAGAGATC
BsaI 1556 GAGACC GGTCTC(1/5)
3566 GAGACC
BsaWI 2880 ACCGGA [AT]^CCGG[AT]
4047 TCCGGA
BspLU11I 4891 ACATGT A^CATGT
4911 ACATGT
BsrBI 4633 CCGCTC CCGCTC(-3/-3)
2024 GAGCGG
BstAPI 1020 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4416 GCAAAGCCTGC
CelII 401 GCTAAGC GC^T[ACGT]AGC
3982 GCTGAGC
DraI 217 TTTAAA TTT^AAA
5026 TTTAAA
DraIII 3448 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4604 CACCGTGTG
EcoNI 2521 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4081 CCTGCCGGAGG
HincII 825 GTCAAC GT[CT]^[GA]AC
3014 GTCAAC
HindII 825 GTCAAC GT[CT]^[GA]AC
3014 GTCAAC
HindIII 3244 AAGCTT A^AGCTT
4748 AAGCTT
KspI 3580 CCGCGG CCGC^GG
3912 CCGCGG
MamI 1477 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATAGAGATC
PvuII 2558 CAGCTG CAG^CTG
3980 CAGCTG
SacII 3580 CCGCGG CCGC^GG
3912 CCGCGG
SapI 2971 GAAGAGC GCTCTTC(1/4)
4663 GCTCTTC
SphI 1701 GCATGC GCATG^C
4233 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflFI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoI XhoII XmnI