################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BbeI BcgI BciVI BfrI BlnI BsiWI Bsp1407I BspDI BsrGI BssHII BssSI BstBI ClaI CpoI EcoRI EcoRV FseI FspI HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfoI SfuI Sse8387I SspBI SwaI Van91I ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 276 GACGTC GACGT^C AccIII 4047 TCCGGA T^CCGGA AhdI 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3841 GTGCAC G^TGCAC Aor51HI 3786 AGCGCT AGC^GCT ApaLI 3841 GTGCAC G^TGCAC AspEI 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 3956 CACGTG CAC^GTG BseAI 4047 TCCGGA T^CCGGA BsiEI 4354 CGGCCG CG[GA][CT]^CG BspEI 4047 TCCGGA T^CCGGA BspHI 990 TCATGA T^CATGA BsrDI 4701 GCAATG GCAATG(2/0) Bst1107I 4330 GTATAC GTA^TAC BstEII 1211 GGTCACC G^GT[ACGT]ACC BstPI 1211 GGTCACC G^GT[ACGT]ACC BstZ17I 4330 GTATAC GTA^TAC DrdI 693 GACTCCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4354 CGGCCG C^GGCCG Eam1105I 4067 GACACGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4354 CGGCCG C^GGCCG Eco47III 3786 AGCGCT AGC^GCT Eco52I 4354 CGGCCG C^GGCCG EcoO65I 1211 GGTCACC G^GT[ACGT]ACC EcoT22I 5043 ATGCAT ATGCA^T MroI 4047 TCCGGA T^CCGGA NaeI 1911 GCCGGC GCC^GGC NgoMIV 1911 GCCGGC G^CCGGC NsiI 5043 ATGCAT ATGCA^T PmaCI 3956 CACGTG CAC^GTG PmlI 3956 CACGTG CAC^GTG RcaI 990 TCATGA T^CATGA SacI 4033 GAGCTC GAGCT^C ScaI 5005 AGTACT AGT^ACT SexAI 4025 ACCAGGT A^CC[AT]GGT SfiI 1 GGCCGTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1910 CGCCGGCG C[GA]^CCGG[CT]G SmaI 3969 CCCGGG CCC^GGG SnaBI 165 TACGTA TAC^GTA SpeI 4818 ACTAGT A^CTAGT SspI 5110 AATATT AAT^ATT XbaI 280 TCTAGA T^CTAGA XmaCI 3969 CCCGGG C^CCGGG XmaI 3969 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1832 GTCTAC GT^[AC][GT]AC 4330 GTATAC ApaI 481 GGGCCC GGGCC^C 4371 GGGCCC BamHI 1429 GGATCC G^GATCC 2509 GGATCC BbvCI 3715 CCTCAGC CCTCAGC(-5/-2) 4782 CCTCAGC BglI 2 GCCGTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2651 GCCCGTCTGGC BlpI 401 GCTAAGC GC^T[ACGT]AGC 3982 GCTGAGC Bpu1102I 401 GCTAAGC GC^T[ACGT]AGC 3982 GCTGAGC BsaAI 165 TACGTA [CT]AC^GT[GA] 3956 CACGTG BsaBI 1477 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3355 GATAGAGATC BsaI 1556 GAGACC GGTCTC(1/5) 3566 GAGACC BsaWI 2880 ACCGGA [AT]^CCGG[AT] 4047 TCCGGA BspLU11I 4891 ACATGT A^CATGT 4911 ACATGT BsrBI 4633 CCGCTC CCGCTC(-3/-3) 2024 GAGCGG BstAPI 1020 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4416 GCAAAGCCTGC CelII 401 GCTAAGC GC^T[ACGT]AGC 3982 GCTGAGC DraI 217 TTTAAA TTT^AAA 5026 TTTAAA DraIII 3448 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG 4604 CACCGTGTG EcoNI 2521 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4081 CCTGCCGGAGG HincII 825 GTCAAC GT[CT]^[GA]AC 3014 GTCAAC HindII 825 GTCAAC GT[CT]^[GA]AC 3014 GTCAAC HindIII 3244 AAGCTT A^AGCTT 4748 AAGCTT KspI 3580 CCGCGG CCGC^GG 3912 CCGCGG MamI 1477 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3355 GATAGAGATC PvuII 2558 CAGCTG CAG^CTG 3980 CAGCTG SacII 3580 CCGCGG CCGC^GG 3912 CCGCGG SapI 2971 GAAGAGC GCTCTTC(1/4) 4663 GCTCTTC SphI 1701 GCATGC GCATG^C 4233 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflFI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoI XhoII XmnI