##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AcyI AhdI AocI
Aor51HI ApaI AscI AspEI AviII
AvrII BbeI BbrPI BcgI BglI
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII BstEII BstPI
Bsu36I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FseI FspI
HgaI Hin1I KasI KspI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NotI NruI PacI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 362 GGTACC G^GTACC
AccII 3498 CGCG CG^CG
AclI 4366 AACGTT AA^CGTT
AgeI 223 ACCGGT A^CCGGT
Alw44I 1882 GTGCAC G^TGCAC
ApaLI 1882 GTGCAC G^TGCAC
Asp718I 362 GGTACC G^GTACC
AspI 1757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3925 GGATCC G^GATCC
BanI 362 GGTACC G^G[CT][GA]CC
BciVI 2499 GGATAC GTATCC(6/5)
BglII 3026 AGATCT A^GATCT
BsaAI 4590 CACGTA [CT]AC^GT[GA]
BsaWI 223 ACCGGT [AT]^CCGG[AT]
BsrDI 62 GCAATG GCAATG(2/0)
BsrFI 223 ACCGGT [GA]^CCGG[CT]
BssSI 4302 CTCGTG CACGAG(-5/-1)
Bst1107I 3795 GTATAC GTA^TAC
BstUI 3498 CGCG CG^CG
BstZ17I 3795 GTATAC GTA^TAC
Cfr10I 223 ACCGGT [GA]^CCGG[CT]
DraII 1108 GGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 76 GACAGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4282 CCGTGG C^C[GA][CT]GG
EcoO109I 1108 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 557 GAATTC G^AATTC
EcoT14I 2823 CCTTGG C^C[AT][AT]GG
HaeII 1261 AGCGCC [GA]GCGC^[CT]
HindIII 200 AAGCTT A^AGCTT
KpnI 362 GGTACC GGTAC^C
MvnI 3498 CGCG CG^CG
PaeR7I 2236 CTCGAG C^TCGAG
PflFI 1757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 223 ACCGGT A^CCGGT
PpuMI 1108 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2964 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4366 AACGTT AA^CGTT
SexAI 3337 ACCAGGT A^CC[AT]GGT
SmaI 2753 CCCGGG CCC^GGG
StyI 2823 CCTTGG C^C[AT][AT]GG
Tth111I 1757 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4966 CCAGATGTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2236 CTCGAG C^TCGAG
XmaCI 2753 CCCGGG C^CCGGG
XmaI 2753 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AvaI 2236 CTCGAG C^[CT]CG[GA]G
2753 CCCGGG
BalI 1940 TGGCCA TGG^CCA
3105 TGGCCA
BbsI 3677 GAAGAC GAAGAC(2/6)
4021 GAAGAC
BbvCI 1984 CCTCAGC CCTCAGC(-5/-2)
2729 GCTGAGG
BcnI 2753 CCCGG CC^[GC]GG
2754 CCGGG
BpuAI 3677 GAAGAC GAAGAC(2/6)
4021 GAAGAC
BseRI 3287 CTCCTC GAGGAG(10/8)
4290 CTCCTC
BsoBI 2236 CTCGAG C^[CT]CG[GA]G
2753 CCCGGG
Bsp1407I 2119 TGTACA T^GTACA
4407 TGTACA
BspMI 3192 ACCTGC ACCTGC(4/8)
3681 ACCTGC
BsrGI 2119 TGTACA T^GTACA
4407 TGTACA
BstAPI 1695 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1814 GCAGCCTCTGC
BstBI 624 TTCGAA TT^CGAA
1134 TTCGAA
DraIII 3643 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4278 CACCCCGTG
EaeI 1940 TGGCCA [CT]^GGCC[GA]
3105 TGGCCA
EcoT22I 1384 ATGCAT ATGCA^T
5041 ATGCAT
HapII 224 CCGG C^CGG
2754 CCGG
HpaII 224 CCGG C^CGG
2754 CCGG
MfeI 1024 CAATTG C^AATTG
3331 CAATTG
MluNI 1940 TGGCCA TGG^CCA
3105 TGGCCA
MscI 1940 TGGCCA TGG^CCA
3105 TGGCCA
MspI 224 CCGG C^CGG
2754 CCGG
MunI 1024 CAATTG C^AATTG
3331 CAATTG
NciI 2753 CCCGG CC^[GC]GG
2754 CCGGG
NdeI 146 CATATG CA^TATG
630 CATATG
NheI 380 GCTAGC G^CTAGC
3162 GCTAGC
NsiI 1384 ATGCAT ATGCA^T
5041 ATGCAT
NspV 624 TTCGAA TT^CGAA
1134 TTCGAA
PstI 921 CTGCAG CTGCA^G
4310 CTGCAG
SapI 1137 GAAGAGC GCTCTTC(1/4)
2434 GAAGAGC
SfuI 624 TTCGAA TT^CGAA
1134 TTCGAA
SspBI 2119 TGTACA T^GTACA
4407 TGTACA
XbaI 205 TCTAGA T^CTAGA
4553 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AseI AsnI Asp700I AspHI AvaII
BanII BbvI BclI BfaI BfrI
BmyI BpmI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspLU11I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoNI EcoRII FbaI
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HpaI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI