##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccIII AflII AflIII AscI
Asp718I AviII AvrII BbeI BfrI
BlnI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrBI BssHII ClaI
CpoI FspI KasI KpnI KspI
MluI MroI NarI NcoI NotI
NruI PacI PmeI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 2400 GACGTC GACGT^C
AccI 3668 GTATAC GT^[AC][GT]AC
AclI 3486 AACGTT AA^CGTT
AgeI 1299 ACCGGT A^CCGGT
AhdI 4210 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 5521 GTGCAC G^TGCAC
Aor51HI 1867 AGCGCT AGC^GCT
ApaLI 5521 GTGCAC G^TGCAC
AseI 4589 ATTAAT AT^TAAT
AsnI 4589 ATTAAT AT^TAAT
AspEI 4210 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 508 CTCGAG C^[CT]CG[GA]G
BalI 4411 TGGCCA TGG^CCA
BamHI 5208 GGATCC G^GATCC
BanI 1335 GGTGCC G^G[CT][GA]CC
BbsI 2187 GAAGAC GAAGAC(2/6)
BbvCI 3333 GCTGAGG CCTCAGC(-5/-2)
BcgI 2083 GCAACTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4474 GGATAC GTATCC(6/5)
BlpI 1853 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1853 GCTGAGC GC^T[ACGT]AGC
BpuAI 2187 GAAGAC GAAGAC(2/6)
BsiEI 1806 CGGCCG CG[GA][CT]^CG
BsmI 2604 GAATGC GAATGC(1/-1)
BsoBI 508 CTCGAG C^[CT]CG[GA]G
Bst1107I 3668 GTATAC GTA^TAC
BstBI 5494 TTCGAA TT^CGAA
BstEII 526 GGTCACC G^GT[ACGT]ACC
BstPI 526 GGTCACC G^GT[ACGT]ACC
BstZ17I 3668 GTATAC GTA^TAC
CelII 1853 GCTGAGC GC^T[ACGT]AGC
EagI 1806 CGGCCG C^GGCCG
Eam1105I 4210 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1806 CGGCCG C^GGCCG
Eco47III 1867 AGCGCT AGC^GCT
Eco52I 1806 CGGCCG C^GGCCG
EcoNI 2090 CCTCGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 526 GGTCACC G^GT[ACGT]ACC
FseI 1807 GGCCGGCC GGCCGG^CC
MluNI 4411 TGGCCA TGG^CCA
MscI 4411 TGGCCA TGG^CCA
NaeI 1808 GCCGGC GCC^GGC
NgoMIV 1808 GCCGGC G^CCGGC
NheI 2585 GCTAGC G^CTAGC
NspV 5494 TTCGAA TT^CGAA
PaeR7I 508 CTCGAG C^TCGAG
PinAI 1299 ACCGGT A^CCGGT
PshBI 4589 ATTAAT AT^TAAT
Psp1406I 3486 AACGTT AA^CGTT
ScaI 3175 AGTACT AGT^ACT
SfuI 5494 TTCGAA TT^CGAA
SwaI 5686 ATTTAAAT ATTT^AAAT
XbaI 2984 TCTAGA T^CTAGA
XhoI 508 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 165 CCTAAGG CC^T[ACGT]AGG
594 CCTGAGG
BbrPI 2679 CACGTG CAC^GTG
2804 CACGTG
BsaAI 2679 CACGTG [CT]AC^GT[GA]
2804 CACGTG
BsaBI 4223 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5734 GATATCAATC
BsaWI 1299 ACCGGT [AT]^CCGG[AT]
4146 ACCGGA
BsrFI 1299 ACCGGT [GA]^CCGG[CT]
1808 GCCGGC
Bsu36I 165 CCTAAGG CC^T[ACGT]AGG
594 CCTGAGG
Cfr10I 1299 ACCGGT [GA]^CCGG[CT]
1808 GCCGGC
DraIII 59 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1657 CACCCAGTG
DrdI 669 GACAACCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1986 GACAAGGCTGTC
DsaI 830 CCACGG C^C[GA][CT]GG
3115 CCGTGG
Eco81I 165 CCTAAGG CC^T[ACGT]AGG
594 CCTGAGG
EcoRV 2071 GATATC GAT^ATC
5734 GATATC
HaeII 1867 AGCGCT [GA]GCGC^[CT]
2935 AGCGCC
HindIII 4947 AAGCTT A^AGCTT
5614 AAGCTT
HpaI 3930 GTTAAC GTT^AAC
3968 GTTAAC
MamI 4223 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5734 GATATCAATC
MfeI 5726 CAATTG C^AATTG
5746 CAATTG
MunI 5726 CAATTG C^AATTG
5746 CAATTG
NdeI 3700 CATATG CA^TATG
3708 CATATG
PflMI 987 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3134 CCAGCTCCTGG
PmaCI 2679 CACGTG CAC^GTG
2804 CACGTG
PmlI 2679 CACGTG CAC^GTG
2804 CACGTG
SmlI 508 CTCGAG C^T[CT][GA]AG
4276 CTCAAG
SspI 27 AATATT AAT^ATT
5691 AATATT
Van91I 987 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3134 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AspI AvaII BanII
BbvI BclI BcnI BfaI BglI
BglII BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflFI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmnI