################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AflII AgeI ApaI AscI Asp718I AspI AviII AvrII BanI BbeI BbrPI BcgI BfrI BlnI BsiEI BsiWI BspDI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PvuI SacII SalI SbfI SfoI SfuI SgrAI SpeI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 2049 GTAGAC GT^[AC][GT]AC AccIII 229 TCCGGA T^CCGGA AclI 1759 AACGTT AA^CGTT AhdI 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3084 AGCGCT AGC^GCT AseI 563 ATTAAT AT^TAAT AsnI 563 ATTAAT AT^TAAT AspEI 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3574 GGATCC G^GATCC BsaI 2883 GAGACC GGTCTC(1/5) BseAI 229 TCCGGA T^CCGGA BsmBI 2085 CGTCTC CGTCTC(1/5) BspEI 229 TCCGGA T^CCGGA BsrDI 1234 GCAATG GCAATG(2/0) BssSI 3307 CTCGTG CACGAG(-5/-1) BstAPI 5026 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 1116 CGGTCCG CG^G[AT]CCG Eam1105I 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3084 AGCGCT AGC^GCT HindIII 2178 AAGCTT A^AGCTT HpaI 3812 GTTAAC GTT^AAC MroI 229 TCCGGA T^CCGGA NcoI 86 CCATGG C^CATGG NdeI 1774 CATATG CA^TATG PaeR7I 3270 CTCGAG C^TCGAG PshAI 3534 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 563 ATTAAT AT^TAAT Psp1406I 1759 AACGTT AA^CGTT RsrII 1116 CGGTCCG CG^G[AT]CCG ScaI 4485 AGTACT AGT^ACT SexAI 3013 ACCTGGT A^CC[AT]GGT SfiI 3335 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 4521 CCCGGG CCC^GGG SnaBI 4347 TACGTA TAC^GTA XhoI 3270 CTCGAG C^TCGAG XmaCI 4521 CCCGGG C^CCGGG XmaI 4521 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 4019 GACGCC G[GA]^CG[CT]C 4413 GGCGTC Alw44I 3998 GTGCAC G^TGCAC 4630 GTGCAC AocI 197 CCTAAGG CC^T[ACGT]AGG 3597 CCTGAGG ApaLI 3998 GTGCAC G^TGCAC 4630 GTGCAC BbsI 851 GAAGAC GAAGAC(2/6) 1769 GAAGAC BciVI 1627 GTATCC GTATCC(6/5) 4261 GTATCC BglI 3336 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5061 GCCCGGAAGGC BlpI 3143 GCTGAGC GC^T[ACGT]AGC 3509 GCTCAGC Bpu1102I 3143 GCTGAGC GC^T[ACGT]AGC 3509 GCTCAGC BpuAI 851 GAAGAC GAAGAC(2/6) 1769 GAAGAC BsaAI 2185 TACGTG [CT]AC^GT[GA] 4347 TACGTA BsaHI 4019 GACGCC G[GA]^CG[CT]C 4413 GGCGTC BsaWI 229 TCCGGA [AT]^CCGG[AT] 286 ACCGGA Bsp1407I 522 TGTACA T^GTACA 1543 TGTACA BspHI 1531 TCATGA T^CATGA 2125 TCATGA BspMI 3244 ACCTGC ACCTGC(4/8) 3044 GCAGGT BsrGI 522 TGTACA T^GTACA 1543 TGTACA BstXI 1390 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4410 CCAGGCGTCTGG Bsu36I 197 CCTAAGG CC^T[ACGT]AGG 3597 CCTGAGG CelII 3143 GCTGAGC GC^T[ACGT]AGC 3509 GCTCAGC Eco81I 197 CCTAAGG CC^T[ACGT]AGG 3597 CCTGAGG Hin1I 4019 GACGCC G[GA]^CG[CT]C 4413 GGCGTC RcaI 1531 TCATGA T^CATGA 2125 TCATGA SapI 375 GAAGAGC GCTCTTC(1/4) 773 GAAGAGC SspBI 522 TGTACA T^GTACA 1543 TGTACA SspI 1157 AATATT AAT^ATT 1964 AATATT StuI 4771 AGGCCT AGG^CCT 5103 AGGCCT XbaI 299 TCTAGA T^CTAGA 350 TCTAGA XcmI 880 CCATTCCTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5036 CCAGTGCAGATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbvCI BbvI BclI BcnI BfaI BglII BmyI BpmI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI