##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AflII AgeI ApaI
AscI Asp718I AspI AviII AvrII
BanI BbeI BbrPI BcgI BfrI
BlnI BsiEI BsiWI BspDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI FspI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PvuI SacII
SalI SbfI SfoI SfuI SgrAI
SpeI SphI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 2049 GTAGAC GT^[AC][GT]AC
AccIII 229 TCCGGA T^CCGGA
AclI 1759 AACGTT AA^CGTT
AhdI 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3084 AGCGCT AGC^GCT
AseI 563 ATTAAT AT^TAAT
AsnI 563 ATTAAT AT^TAAT
AspEI 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3574 GGATCC G^GATCC
BsaI 2883 GAGACC GGTCTC(1/5)
BseAI 229 TCCGGA T^CCGGA
BsmBI 2085 CGTCTC CGTCTC(1/5)
BspEI 229 TCCGGA T^CCGGA
BsrDI 1234 GCAATG GCAATG(2/0)
BssSI 3307 CTCGTG CACGAG(-5/-1)
BstAPI 5026 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 1116 CGGTCCG CG^G[AT]CCG
Eam1105I 2310 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3084 AGCGCT AGC^GCT
HindIII 2178 AAGCTT A^AGCTT
HpaI 3812 GTTAAC GTT^AAC
MroI 229 TCCGGA T^CCGGA
NcoI 86 CCATGG C^CATGG
NdeI 1774 CATATG CA^TATG
PaeR7I 3270 CTCGAG C^TCGAG
PshAI 3534 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 563 ATTAAT AT^TAAT
Psp1406I 1759 AACGTT AA^CGTT
RsrII 1116 CGGTCCG CG^G[AT]CCG
ScaI 4485 AGTACT AGT^ACT
SexAI 3013 ACCTGGT A^CC[AT]GGT
SfiI 3335 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4521 CCCGGG CCC^GGG
SnaBI 4347 TACGTA TAC^GTA
XhoI 3270 CTCGAG C^TCGAG
XmaCI 4521 CCCGGG C^CCGGG
XmaI 4521 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 4019 GACGCC G[GA]^CG[CT]C
4413 GGCGTC
Alw44I 3998 GTGCAC G^TGCAC
4630 GTGCAC
AocI 197 CCTAAGG CC^T[ACGT]AGG
3597 CCTGAGG
ApaLI 3998 GTGCAC G^TGCAC
4630 GTGCAC
BbsI 851 GAAGAC GAAGAC(2/6)
1769 GAAGAC
BciVI 1627 GTATCC GTATCC(6/5)
4261 GTATCC
BglI 3336 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5061 GCCCGGAAGGC
BlpI 3143 GCTGAGC GC^T[ACGT]AGC
3509 GCTCAGC
Bpu1102I 3143 GCTGAGC GC^T[ACGT]AGC
3509 GCTCAGC
BpuAI 851 GAAGAC GAAGAC(2/6)
1769 GAAGAC
BsaAI 2185 TACGTG [CT]AC^GT[GA]
4347 TACGTA
BsaHI 4019 GACGCC G[GA]^CG[CT]C
4413 GGCGTC
BsaWI 229 TCCGGA [AT]^CCGG[AT]
286 ACCGGA
Bsp1407I 522 TGTACA T^GTACA
1543 TGTACA
BspHI 1531 TCATGA T^CATGA
2125 TCATGA
BspMI 3244 ACCTGC ACCTGC(4/8)
3044 GCAGGT
BsrGI 522 TGTACA T^GTACA
1543 TGTACA
BstXI 1390 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4410 CCAGGCGTCTGG
Bsu36I 197 CCTAAGG CC^T[ACGT]AGG
3597 CCTGAGG
CelII 3143 GCTGAGC GC^T[ACGT]AGC
3509 GCTCAGC
Eco81I 197 CCTAAGG CC^T[ACGT]AGG
3597 CCTGAGG
Hin1I 4019 GACGCC G[GA]^CG[CT]C
4413 GGCGTC
RcaI 1531 TCATGA T^CATGA
2125 TCATGA
SapI 375 GAAGAGC GCTCTTC(1/4)
773 GAAGAGC
SspBI 522 TGTACA T^GTACA
1543 TGTACA
SspI 1157 AATATT AAT^ATT
1964 AATATT
StuI 4771 AGGCCT AGG^CCT
5103 AGGCCT
XbaI 299 TCTAGA T^CTAGA
350 TCTAGA
XcmI 880 CCATTCCTGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5036 CCAGTGCAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanII
BbvCI BbvI BclI BcnI BfaI
BglII BmyI BpmI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI