##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I ApaLI AscI Asp700I Asp718I
AspEI AspI BalI BbrPI BcgI
BclI BsaAI BsaBI BseAI BsiWI
BspDI BspEI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I EcoRI
FbaI FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfuI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 1212 GACGTC GACGT^C
AccI 1189 GTCTAC GT^[AC][GT]AC
Aor51HI 1584 AGCGCT AGC^GCT
ApaI 1008 GGGCCC GGGCC^C
AseI 2808 ATTAAT AT^TAAT
AsnI 2808 ATTAAT AT^TAAT
AvrII 2794 CCTAGG C^CTAGG
BbeI 174 GGCGCC GGCGC^C
BbsI 3760 GAAGAC GAAGAC(2/6)
BciVI 3311 GTATCC GTATCC(6/5)
BlnI 2794 CCTAGG C^CTAGG
BlpI 3360 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3360 GCTAAGC GC^T[ACGT]AGC
BpuAI 3760 GAAGAC GAAGAC(2/6)
BsmBI 4687 GAGACG CGTCTC(1/5)
BsmI 3767 GAATGC GAATGC(1/-1)
Bsp1407I 1038 TGTACA T^GTACA
BsrGI 1038 TGTACA T^GTACA
CelII 3360 GCTAAGC GC^T[ACGT]AGC
DraIII 1485 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3669 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1584 AGCGCT AGC^GCT
EcoRV 692 GATATC GAT^ATC
KasI 174 GGCGCC G^GCGCC
NaeI 465 GCCGGC GCC^GGC
NarI 174 GGCGCC GG^CGCC
NgoMIV 465 GCCGGC G^CCGGC
NruI 58 TCGCGA TCG^CGA
PshBI 2808 ATTAAT AT^TAAT
SacI 1623 GAGCTC GAGCT^C
SexAI 2104 ACCAGGT A^CC[AT]GGT
SfoI 174 GGCGCC GGC^GCC
SgrAI 1868 CACCGGCG C[GA]^CCGG[CT]G
SmaI 261 CCCGGG CCC^GGG
SspBI 1038 TGTACA T^GTACA
StuI 1084 AGGCCT AGG^CCT
TaqI 1603 TCGA T^CGA
TthHB8I 1603 TCGA T^CGA
XcmI 3214 CCAGTGAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 261 CCCGGG C^CCGGG
XmaI 261 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 174 GGCGCC G[GA]^CG[CT]C
1212 GACGTC
AfaI 598 GTAC GT^AC
1039 GTAC
AflII 2686 CTTAAG C^TTAAG
4809 CTTAAG
AlwNI 1218 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
4205 CAGAATCTG
AvaI 118 CCCGAG C^[CT]CG[GA]G
261 CCCGGG
AviII 642 TGCGCA TGC^GCA
1845 TGCGCA
BamHI 660 GGATCC G^GATCC
5076 GGATCC
BanI 174 GGCGCC G^G[CT][GA]CC
703 GGTGCC
BfrI 2686 CTTAAG C^TTAAG
4809 CTTAAG
BglII 2732 AGATCT A^GATCT
3010 AGATCT
BsaHI 174 GGCGCC G[GA]^CG[CT]C
1212 GACGTC
BsaI 2165 GAGACC GGTCTC(1/5)
4520 GAGACC
BsaWI 1015 ACCGGA [AT]^CCGG[AT]
1617 ACCGGA
BsiEI 130 CGGCCG CG[GA][CT]^CG
199 CGGCCG
BsoBI 118 CCCGAG C^[CT]CG[GA]G
261 CCCGGG
BspHI 795 TCATGA T^CATGA
4964 TCATGA
BsrBI 1889 GAGCGG CCGCTC(-3/-3)
1973 GAGCGG
BsrFI 465 GCCGGC [GA]^CCGG[CT]
1869 ACCGGC
BstAPI 1148 GCAAAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1821 GCACTGACTGC
Cfr10I 465 GCCGGC [GA]^CCGG[CT]
1869 ACCGGC
EagI 130 CGGCCG C^GGCCG
199 CGGCCG
EclXI 130 CGGCCG C^GGCCG
199 CGGCCG
Eco52I 130 CGGCCG C^GGCCG
199 CGGCCG
EcoNI 586 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4433 CCTAAAGTAGG
EcoT22I 3370 ATGCAT ATGCA^T
3953 ATGCAT
FspI 642 TGCGCA TGC^GCA
1845 TGCGCA
Hin1I 174 GGCGCC G[GA]^CG[CT]C
1212 GACGTC
HincII 2147 GTCAAC GT[CT]^[GA]AC
5086 GTTGAC
HindII 2147 GTCAAC GT[CT]^[GA]AC
5086 GTTGAC
NsiI 3370 ATGCAT ATGCA^T
3953 ATGCAT
PflMI 2169 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4204 CCAGAATCTGG
RcaI 795 TCATGA T^CATGA
4964 TCATGA
RsaI 598 GTAC GT^AC
1039 GTAC
Van91I 2169 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4204 CCAGAATCTGG
XbaI 2161 TCTAGA T^CTAGA
3843 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AflIII AluI
AlwI AocI ApoI AspHI AvaII
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII