##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AlwNI Aor51HI
AscI Asp718I AspEI AvaI AviII
AvrII BciVI BcnI BglI BlnI
BlpI Bpu1102I BsaWI BseAI BsiWI
BsmBI BsoBI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
Cfr10I CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT14I FauI FseI
FspI HapII HgaI HindIII HpaII
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
Psp1406I RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StyI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 4798 GGCGCC G[GA]^CG[CT]C
AflII 1022 CTTAAG C^TTAAG
ApaI 178 GGGCCC GGGCC^C
AseI 4245 ATTAAT AT^TAAT
AsnI 4245 ATTAAT AT^TAAT
BanI 4798 GGCGCC G^G[CT][GA]CC
BbeI 4798 GGCGCC GGCGC^C
BbrPI 1088 CACGTG CAC^GTG
BbvCI 4687 GCTGAGG CCTCAGC(-5/-2)
BfrI 1022 CTTAAG C^TTAAG
BglII 1903 AGATCT A^GATCT
BsaAI 1088 CACGTG [CT]AC^GT[GA]
BsaHI 4798 GGCGCC G[GA]^CG[CT]C
BspDI 3440 ATCGAT AT^CGAT
BstAPI 4389 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3440 ATCGAT AT^CGAT
DraI 4224 TTTAAA TTT^AAA
DrdI 508 GACAGAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 4798 GGCGCC G[GA]^CG[CT]C
HincII 2776 GTTAAC GT[CT]^[GA]AC
HindII 2776 GTTAAC GT[CT]^[GA]AC
HpaI 2776 GTTAAC GTT^AAC
KasI 4798 GGCGCC G^GCGCC
MspA1I 1835 CAGCTG C[AC]G^C[GT]G
NarI 4798 GGCGCC GG^CGCC
NdeI 1528 CATATG CA^TATG
PmaCI 1088 CACGTG CAC^GTG
PmlI 1088 CACGTG CAC^GTG
PshAI 2527 GACTCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4245 ATTAAT AT^TAAT
PstI 2900 CTGCAG CTGCA^G
PvuI 4878 CGATCG CGAT^CG
PvuII 1835 CAGCTG CAG^CTG
SacI 1813 GAGCTC GAGCT^C
ScaI 2220 AGTACT AGT^ACT
SfoI 4798 GGCGCC GGC^GCC
SpeI 4778 ACTAGT A^CTAGT
XcmI 3004 CCAATGTTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 944 ACATGT A^C[GA][CT]GT
1006 ACATGT
Alw44I 2067 GTGCAC G^TGCAC
2177 GTGCAC
AocI 3802 CCTAAGG CC^T[ACGT]AGG
4705 CCTGAGG
ApaLI 2067 GTGCAC G^TGCAC
2177 GTGCAC
BalI 3829 TGGCCA TGG^CCA
4732 TGGCCA
BamHI 1923 GGATCC G^GATCC
3671 GGATCC
BcgI 4110 CGAAGAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3925 GCAGGAGAATCG
BsaBI 905 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3253 GATGTTGATC
BsaI 2963 GAGACC GGTCTC(1/5)
3819 GAGACC
BsiEI 2059 CGGTCG CG[GA][CT]^CG
4878 CGATCG
BspHI 736 TCATGA T^CATGA
2910 TCATGA
BspLU11I 944 ACATGT A^CATGT
1006 ACATGT
BssSI 2529 CTCGTG CACGAG(-5/-1)
4657 CTCGTG
BstF5I 248 CATCC GGATG(2/0)
4682 GGATG
Bsu36I 3802 CCTAAGG CC^T[ACGT]AGG
4705 CCTGAGG
Cac8I 1978 GCAGGC GC[ACGT]^[ACGT]GC
3890 GCGTGC
CfoI 1059 GCGC GCG^C
4799 GCGC
EaeI 3829 TGGCCA [CT]^GGCC[GA]
4732 TGGCCA
Eco81I 3802 CCTAAGG CC^T[ACGT]AGG
4705 CCTGAGG
FokI 248 CATCC GGATG(9/13)
4682 GGATG
HaeII 1058 GGCGCT [GA]GCGC^[CT]
4798 GGCGCC
HhaI 1059 GCGC GCG^C
4799 GCGC
HinP1I 1059 GCGC G^CGC
4799 GCGC
MamI 905 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3253 GATGTTGATC
MluNI 3829 TGGCCA TGG^CCA
4732 TGGCCA
MscI 3829 TGGCCA TGG^CCA
4732 TGGCCA
PflMI 717 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4851 CCAGGAGGTGG
PpuMI 2869 AGGTCCT [GA]G^G[AT]CC[CT]
3069 GGGTCCT
RcaI 736 TCATGA T^CATGA
2910 TCATGA
SapI 389 GAAGAGC GCTCTTC(1/4)
1630 GAAGAGC
SexAI 437 ACCAGGT A^CC[AT]GGT
498 ACCAGGT
SspI 2856 AATATT AAT^ATT
3406 AATATT
Van91I 717 CCATCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4851 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AspI AvaII
BanII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstNI BstXI BstYI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRI EcoRII EcoT22I FbaI
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI