##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AflII AhdI AscI AseI
AsnI AspEI AviII BamHI BbrPI
BfrI BsiWI Bsp1407I BspDI BspLU11I
BsrGI BssHII ClaI CpoI DraI
Eam1105I EcoNI EcoRV FspI HincII
HindII HindIII HpaI MfeI MunI
NdeI NheI NotI NruI PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 830 GGTACC G^GTACC
AccIII 4973 TCCGGA T^CCGGA
AflIII 5725 ACGCGT A^C[GA][CT]GT
AgeI 3411 ACCGGT A^CCGGT
Asp700I 2330 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 830 GGTACC G^GTACC
AspI 678 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4340 CCTAGG C^CTAGG
BciVI 619 GTATCC GTATCC(6/5)
BclI 5840 TGATCA T^GATCA
BglII 5500 AGATCT A^GATCT
BlnI 4340 CCTAGG C^CTAGG
BsaAI 2036 CACGTA [CT]AC^GT[GA]
BsaBI 2198 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4973 TCCGGA T^CCGGA
BsmI 605 GAATGC GAATGC(1/-1)
BspEI 4973 TCCGGA T^CCGGA
Bst1107I 4665 GTATAC GTA^TAC
BstBI 1593 TTCGAA TT^CGAA
BstEII 3414 GGTCACC G^GT[ACGT]ACC
BstPI 3414 GGTCACC G^GT[ACGT]ACC
BstZ17I 4665 GTATAC GTA^TAC
EcoO65I 3414 GGTCACC G^GT[ACGT]ACC
EcoT22I 5823 ATGCAT ATGCA^T
FbaI 5840 TGATCA T^GATCA
FseI 4826 GGCCGGCC GGCCGG^CC
KpnI 830 GGTACC GGTAC^C
KspI 81 CCGCGG CCGC^GG
MamI 2198 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 5725 ACGCGT A^CGCGT
MroI 4973 TCCGGA T^CCGGA
NsiI 5823 ATGCAT ATGCA^T
NspV 1593 TTCGAA TT^CGAA
PflFI 678 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3411 ACCGGT A^CCGGT
PpuMI 3820 AGGACCT [GA]G^G[AT]CC[CT]
SacII 81 CCGCGG CCGC^GG
SfiI 4058 GGCCCGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1593 TTCGAA TT^CGAA
SphI 4454 GCATGC GCATG^C
SspI 4310 AATATT AAT^ATT
TfiI 884 GAATC G^A[AT]TC
Tth111I 678 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2330 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1626 CCTGAGG CC^T[ACGT]AGG
4434 CCTCAGG
Aor51HI 580 AGCGCT AGC^GCT
2248 AGCGCT
BalI 1564 TGGCCA TGG^CCA
5775 TGGCCA
BsaWI 3411 ACCGGT [AT]^CCGG[AT]
4973 TCCGGA
BsmBI 204 CGTCTC CGTCTC(1/5)
5181 CGTCTC
BspHI 757 TCATGA T^CATGA
958 TCATGA
BsrDI 2645 CATTGC GCAATG(2/0)
1987 GCAATG
BstYI 3586 AGATCC [GA]^GATC[CT]
5500 AGATCT
Bsu36I 1626 CCTGAGG CC^T[ACGT]AGG
4434 CCTCAGG
DraIII 2233 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2805 CACGCAGTG
DrdI 936 GACCTCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3482 GACCCACCAGTC
Eco47III 580 AGCGCT AGC^GCT
2248 AGCGCT
Eco81I 1626 CCTGAGG CC^T[ACGT]AGG
4434 CCTCAGG
EcoRI 2180 GAATTC G^AATTC
5107 GAATTC
MflI 3586 AGATCC [GA]^GATC[CT]
5500 AGATCT
MluNI 1564 TGGCCA TGG^CCA
5775 TGGCCA
MscI 1564 TGGCCA TGG^CCA
5775 TGGCCA
PshAI 950 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4525 GACAGACGTC
RcaI 757 TCATGA T^CATGA
958 TCATGA
SmaI 2436 CCCGGG CCC^GGG
4464 CCCGGG
XhoII 3586 AGATCC [GA]^GATC[CT]
5500 AGATCT
XmaCI 2436 CCCGGG C^CCGGG
4464 CCCGGG
XmaI 2436 CCCGGG C^CCGGG
4464 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI