##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI AscI Asp718I AspEI AvrII
BbvCI BcgI BciVI BfrI BlnI
BseAI BsiWI BspEI BspLU11I Bst1107I
BstBI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI HincII HindII
HpaI KpnI MluI MroI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI RsrII
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 4755 AACGTT AA^CGTT
Alw44I 1246 GTGCAC G^TGCAC
AocI 3840 CCTCAGG CC^T[ACGT]AGG
ApaLI 1246 GTGCAC G^TGCAC
AseI 546 ATTAAT AT^TAAT
AsnI 546 ATTAAT AT^TAAT
AviII 2047 TGCGCA TGC^GCA
BalI 4308 TGGCCA TGG^CCA
BamHI 2170 GGATCC G^GATCC
BbrPI 2829 CACGTG CAC^GTG
BclI 3546 TGATCA T^GATCA
BsaWI 60 TCCGGT [AT]^CCGG[AT]
BsiEI 1286 CGATCG CG[GA][CT]^CG
BsmI 1592 GAATGC GAATGC(1/-1)
Bsp1407I 1420 TGTACA T^GTACA
BspDI 1375 ATCGAT AT^CGAT
BspHI 3432 TCATGA T^CATGA
BsrGI 1420 TGTACA T^GTACA
BssSI 2433 CACGAG CACGAG(-5/-1)
Bsu36I 3840 CCTCAGG CC^T[ACGT]AGG
ClaI 1375 ATCGAT AT^CGAT
DraIII 2385 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3840 CCTCAGG CC^T[ACGT]AGG
FbaI 3546 TGATCA T^GATCA
FspI 2047 TGCGCA TGC^GCA
HindIII 3792 AAGCTT A^AGCTT
MfeI 1234 CAATTG C^AATTG
MluNI 4308 TGGCCA TGG^CCA
MscI 4308 TGGCCA TGG^CCA
MunI 1234 CAATTG C^AATTG
NaeI 2778 GCCGGC GCC^GGC
NgoMIV 2778 GCCGGC G^CCGGC
PmaCI 2829 CACGTG CAC^GTG
PmlI 2829 CACGTG CAC^GTG
PshBI 546 ATTAAT AT^TAAT
Psp1406I 4755 AACGTT AA^CGTT
PvuI 1286 CGATCG CGAT^CG
RcaI 3432 TCATGA T^CATGA
SapI 102 GAAGAGC GCTCTTC(1/4)
SmaI 1132 CCCGGG CCC^GGG
SspBI 1420 TGTACA T^GTACA
SspI 4714 AATATT AAT^ATT
XmaCI 1132 CCCGGG C^CCGGG
XmaI 1132 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1681 ACGTGT A^C[GA][CT]GT
1804 ACGTGT
AlwNI 207 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
958 CAGTGACTG
Aor51HI 2320 AGCGCT AGC^GCT
4641 AGCGCT
ApaI 732 GGGCCC GGGCC^C
4212 GGGCCC
Asp700I 47 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2400 GAAGGGGTTC
AspI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4010 GACCAGGTC
BglI 1300 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4111 GCCAGCCAGGC
BglII 120 AGATCT A^GATCT
1030 AGATCT
BsaBI 115 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3547 GATCACCATC
BsmBI 368 CGTCTC CGTCTC(1/5)
2074 GAGACG
BsrBI 833 CCGCTC CCGCTC(-3/-3)
1907 CCGCTC
BsrDI 2869 GCAATG GCAATG(2/0)
2926 GCAATG
BsrFI 2673 ACCGGC [GA]^CCGG[CT]
2778 GCCGGC
BssHII 2055 GCGCGC G^CGCGC
2057 GCGCGC
Cfr10I 2673 ACCGGC [GA]^CCGG[CT]
2778 GCCGGC
Eco47III 2320 AGCGCT AGC^GCT
4641 AGCGCT
MamI 115 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3547 GATCACCATC
PaeR7I 1103 CTCGAG C^TCGAG
2202 CTCGAG
PflFI 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4010 GACCAGGTC
SacI 450 GAGCTC GAGCT^C
2756 GAGCTC
SfiI 1299 GGCCTGAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4110 GGCCAGCCAGGCC
SphI 2371 GCATGC GCATG^C
3670 GCATGC
StuI 3040 AGGCCT AGG^CCT
3815 AGGCCT
Tth111I 825 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4010 GACCAGGTC
XcmI 3090 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3209 CCATGGGGGTAGTGG
XhoI 1103 CTCGAG C^TCGAG
2202 CTCGAG
XmnI 47 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2400 GAAGGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII