##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AlwNI AscI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BspEI BspHI BssHII BssSI BstAPI
BstBI BstEII BstPI CpoI DraII
DrdI DsaI EcoNI EcoO109I EcoO65I
FbaI FseI FspI Hin1I KasI
KpnI KspI MluI MroI NarI
NcoI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 71 enzymes) #
###################################
AccI 1795 GTATAC GT^[AC][GT]AC
AclI 3204 AACGTT AA^CGTT
AhdI 3637 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2270 GTGCAC G^TGCAC
AocI 1401 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2447 AGCGCT AGC^GCT
ApaI 266 GGGCCC GGGCC^C
ApaLI 2270 GTGCAC G^TGCAC
AseI 597 ATTAAT AT^TAAT
AsnI 597 ATTAAT AT^TAAT
Asp700I 326 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3637 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 261 GGTCC G^G[AT]CC
BalI 458 TGGCCA TGG^CCA
BanI 1723 GGCACC G^G[CT][GA]CC
BanII 266 GGGCCC G[GA]GC[CT]^C
BbsI 330 GTCTTC GAAGAC(2/6)
BcgI 548 GCACAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2682 GGATAC GTATCC(6/5)
BglI 370 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1744 AGATCT A^GATCT
BlpI 999 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 999 GCTGAGC GC^T[ACGT]AGC
BpuAI 330 GTCTTC GAAGAC(2/6)
BsaBI 1739 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 19 CGGCCG CG[GA][CT]^CG
BspDI 1337 ATCGAT AT^CGAT
BsrBI 2691 CCGCTC CCGCTC(-3/-3)
BsrFI 63 GCCGGC [GA]^CCGG[CT]
Bst1107I 1795 GTATAC GTA^TAC
BstF5I 3122 GGATG GGATG(2/0)
BstZ17I 1795 GTATAC GTA^TAC
Bsu36I 1401 CCTTAGG CC^T[ACGT]AGG
CelII 999 GCTGAGC GC^T[ACGT]AGC
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
ClaI 1337 ATCGAT AT^CGAT
EagI 19 CGGCCG C^GGCCG
Eam1105I 3637 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 19 CGGCCG C^GGCCG
Eco47III 2447 AGCGCT AGC^GCT
Eco52I 19 CGGCCG C^GGCCG
Eco81I 1401 CCTTAGG CC^T[ACGT]AGG
EcoRI 1948 GAATTC G^AATTC
EcoRV 2195 GATATC GAT^ATC
EcoT22I 847 ATGCAT ATGCA^T
FauI 3421 CCCGC CCCGC(4/6)
FokI 3122 GGATG GGATG(9/13)
HaeII 2447 AGCGCT [GA]GCGC^[CT]
HincII 2083 GTTAAC GT[CT]^[GA]AC
HindII 2083 GTTAAC GT[CT]^[GA]AC
HindIII 2887 AAGCTT A^AGCTT
HpaI 2083 GTTAAC GTT^AAC
MamI 1739 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1224 CAATTG C^AATTG
MluNI 458 TGGCCA TGG^CCA
MscI 458 TGGCCA TGG^CCA
MspA1I 221 CAGCGG C[AC]G^C[GT]G
MunI 1224 CAATTG C^AATTG
NaeI 63 GCCGGC GCC^GGC
NgoMIV 63 GCCGGC G^CCGGC
NheI 2146 GCTAGC G^CTAGC
NsiI 847 ATGCAT ATGCA^T
PflMI 3080 CCATATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 597 ATTAAT AT^TAAT
Psp1406I 3204 AACGTT AA^CGTT
SapI 2693 GCTCTTC GCTCTTC(1/4)
SpeI 1663 ACTAGT A^CTAGT
SwaI 1965 ATTTAAAT ATTT^AAAT
Van91I 3080 CCATATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2827 TCTAGA T^CTAGA
XmnI 326 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 23 CGCG CG^CG
71 CGCG
AflII 1953 CTTAAG C^TTAAG
2306 CTTAAG
BfrI 1953 CTTAAG C^TTAAG
2306 CTTAAG
BsmAI 112 GAGAC GTCTC(1/5)
1202 GTCTC
BsmI 845 GAATGC GAATGC(1/-1)
2325 GAATGC
BspLU11I 919 ACATGT A^CATGT
1008 ACATGT
BspMI 10 ACCTGC ACCTGC(4/8)
2247 ACCTGC
BstUI 23 CGCG CG^CG
71 CGCG
DraIII 1872 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2066 CACTGTGTG
EaeI 19 CGGCCG [CT]^GGCC[GA]
458 TGGCCA
EcoT14I 1316 CCTTGG C^C[AT][AT]GG
2678 CCTTGG
HgaI 298 GACGC GACGC(5/10)
2433 GACGC
MvnI 23 CGCG CG^CG
71 CGCG
NdeI 3276 CATATG CA^TATG
3394 CATATG
SmaI 189 CCCGGG CCC^GGG
269 CCCGGG
SmlI 1953 CTTAAG C^T[CT][GA]AG
2306 CTTAAG
SspI 1921 AATATT AAT^ATT
3460 AATATT
StuI 384 AGGCCT AGG^CCT
1244 AGGCCT
StyI 1316 CCTTGG C^C[AT][AT]GG
2678 CCTTGG
TfiI 2098 GATTC G^A[AT]TC
2785 GATTC
XcmI 2615 CCATTGTCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3351 CCAAAAACTAAATGG
XmaCI 189 CCCGGG C^CCGGG
269 CCCGGG
XmaI 189 CCCGGG C^CCGGG
269 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SspBI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII