##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BstBI CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI KspI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I StuI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 108 GTATAC GT^[AC][GT]AC
AccII 1671 CGCG CG^CG
AclI 3546 AACGTT AA^CGTT
AlwNI 3012 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2029 CCTTAGG CC^T[ACGT]AGG
AspHI 578 GAGCTC G[AT]GC[AT]^C
BalI 2869 TGGCCA TGG^CCA
BamHI 762 GGATCC G^GATCC
BanI 1661 GGTGCC G^G[CT][GA]CC
BbrPI 126 CACGTG CAC^GTG
BbvCI 1715 CCTCAGC CCTCAGC(-5/-2)
BciVI 158 GTATCC GTATCC(6/5)
BcnI 1693 CCCGG CC^[GC]GG
BglII 3086 AGATCT A^GATCT
BpmI 1949 CTCCAG CTGGAG(16/14)
BsaAI 126 CACGTG [CT]AC^GT[GA]
BseRI 575 GAGGAG GAGGAG(10/8)
BsiHKAI 578 GAGCTC G[AT]GC[AT]^C
BsmFI 1425 GTCCC GGGAC(10/14)
BspLU11I 3530 ACATGT A^CATGT
Bst1107I 108 GTATAC GTA^TAC
BstEII 1471 GGTGACC G^GT[ACGT]ACC
BstPI 1471 GGTGACC G^GT[ACGT]ACC
BstUI 1671 CGCG CG^CG
BstXI 1725 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 108 GTATAC GTA^TAC
Bsu36I 2029 CCTTAGG CC^T[ACGT]AGG
DsaI 174 CCATGG C^C[GA][CT]GG
EaeI 2869 TGGCCA [CT]^GGCC[GA]
Eco81I 2029 CCTTAGG CC^T[ACGT]AGG
EcoNI 1175 CCTTTAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1471 GGTGACC G^GT[ACGT]ACC
EcoT22I 3309 ATGCAT ATGCA^T
FauI 1294 GCGGG CCCGC(4/6)
HaeIII 2870 GGCC GG^CC
HapII 1694 CCGG C^CGG
HgaI 1672 GCGTC GACGC(5/10)
HpaI 3208 GTTAAC GTT^AAC
HpaII 1694 CCGG C^CGG
MluI 1670 ACGCGT A^CGCGT
MluNI 2869 TGGCCA TGG^CCA
MscI 2869 TGGCCA TGG^CCA
MspI 1694 CCGG C^CGG
MvnI 1671 CGCG CG^CG
NciI 1693 CCCGG CC^[GC]GG
NcoI 174 CCATGG C^CATGG
NdeI 3632 CATATG CA^TATG
NsiI 3309 ATGCAT ATGCA^T
NspI 3530 ACATGT [GA]CATG^[CT]
PflMI 323 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 126 CACGTG CAC^GTG
PmlI 126 CACGTG CAC^GTG
Psp1406I 3546 AACGTT AA^CGTT
SacI 578 GAGCTC GAGCT^C
SfaNI 1929 GCATC GCATC(5/9)
SmlI 1353 CTTGAG C^T[CT][GA]AG
SpeI 727 ACTAGT A^CTAGT
SwaI 853 ATTTAAAT ATTT^AAAT
Van91I 323 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1017 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 1670 ACGCGT A^C[GA][CT]GT
3530 ACATGT
BsaBI 673 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4096 GATATACATC
BsaI 1622 GAGACC GGTCTC(1/5)
1170 GGTCTC
BsgI 1866 CTGCAC GTGCAG(16/14)
1654 GTGCAG
BsmI 3307 GAATGC GAATGC(1/-1)
3311 GCATTC
BsrDI 3337 CATTGC GCAATG(2/0)
702 GCAATG
BssSI 398 CTCGTG CACGAG(-5/-1)
1751 CTCGTG
BstAPI 1680 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3359 GCATATTTTGC
BstYI 762 GGATCC [GA]^GATC[CT]
3086 AGATCT
DraII 61 AGGTCCT [GA]G^G[ACGT]CC[CT]
1413 AGGACCC
EcoO109I 61 AGGTCCT [GA]G^G[ACGT]CC[CT]
1413 AGGACCC
MamI 673 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4096 GATATACATC
MfeI 789 CAATTG C^AATTG
2221 CAATTG
MflI 762 GGATCC [GA]^GATC[CT]
3086 AGATCT
MunI 789 CAATTG C^AATTG
2221 CAATTG
NheI 357 GCTAGC G^CTAGC
648 GCTAGC
PpuMI 61 AGGTCCT [GA]G^G[AT]CC[CT]
1413 AGGACCC
PshAI 131 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1624 GACCAGAGTC
PstI 1649 CTGCAG CTGCA^G
2044 CTGCAG
ScaI 724 AGTACT AGT^ACT
2275 AGTACT
XhoII 762 GGATCC [GA]^GATC[CT]
3086 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AvaII
BanII BbsI BbvI BclI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PshBI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfcI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI