##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
BanI BbeI BbrPI BcgI BcnI
BglI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FseI FspI HaeII
Hin1I KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 53 CGCG CG^CG
AgeI 43 ACCGGT A^CCGGT
AvrII 2148 CCTAGG C^CTAGG
BalI 2960 TGGCCA TGG^CCA
BamHI 643 GGATCC G^GATCC
BanII 1180 GAGCTC G[GA]GC[CT]^C
BbvCI 1684 CCTCAGC CCTCAGC(-5/-2)
BbvI 723 GCAGC GCAGC(8/12)
BglII 1174 AGATCT A^GATCT
BlnI 2148 CCTAGG C^CTAGG
BsaAI 1257 TACGTG [CT]AC^GT[GA]
BsaBI 638 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 43 ACCGGT [AT]^CCGG[AT]
BseRI 3733 CTCCTC GAGGAG(10/8)
BsgI 92 GTGCAG GTGCAG(16/14)
Bsp1407I 1281 TGTACA T^GTACA
BsrDI 2696 GCAATG GCAATG(2/0)
BsrFI 43 ACCGGT [GA]^CCGG[CT]
BsrGI 1281 TGTACA T^GTACA
BstUI 53 CGCG CG^CG
CfoI 54 GCGC GCG^C
Cfr10I 43 ACCGGT [GA]^CCGG[CT]
DsaI 1050 CCACGG C^C[GA][CT]GG
EcoT14I 2148 CCTAGG C^C[AT][AT]GG
EcoT22I 3783 ATGCAT ATGCA^T
HapII 44 CCGG C^CGG
HgaI 72 GCGTC GACGC(5/10)
HhaI 54 GCGC GCG^C
HinP1I 54 GCGC G^CGC
HpaII 44 CCGG C^CGG
MamI 638 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2960 TGGCCA TGG^CCA
MscI 2960 TGGCCA TGG^CCA
MspI 44 CCGG C^CGG
MvnI 53 CGCG CG^CG
NdeI 1892 CATATG CA^TATG
NsiI 3783 ATGCAT ATGCA^T
PflMI 2119 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 43 ACCGGT A^CCGGT
PstI 32 CTGCAG CTGCA^G
SacI 1180 GAGCTC GAGCT^C
ScaI 991 AGTACT AGT^ACT
SexAI 577 ACCTGGT A^CC[AT]GGT
SspBI 1281 TGTACA T^GTACA
SspI 2062 AATATT AAT^ATT
StyI 2148 CCTAGG C^C[AT][AT]GG
TseI 723 GCAGC G^C[AT]GC
Van91I 2119 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3433 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2410 ACATGT A^C[GA][CT]GT
4007 ACATGT
AlwNI 2120 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
3479 CAGTCTCTG
AocI 665 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
BclI 105 TGATCA T^GATCA
3722 TGATCA
BspHI 3627 TCATGA T^CATGA
4031 TCATGA
BspLU11I 2410 ACATGT A^CATGT
4007 ACATGT
BspMI 30 ACCTGC ACCTGC(4/8)
2087 GCAGGT
Bst1107I 2774 GTATAC GTA^TAC
3256 GTATAC
BstZ17I 2774 GTATAC GTA^TAC
3256 GTATAC
Bsu36I 665 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
EaeI 674 CGGCCA [CT]^GGCC[GA]
2960 TGGCCA
Eco81I 665 CCTGAGG CC^T[ACGT]AGG
3299 CCTTAGG
FbaI 105 TGATCA T^GATCA
3722 TGATCA
HindIII 1119 AAGCTT A^AGCTT
3940 AAGCTT
HpaI 1919 GTTAAC GTT^AAC
2740 GTTAAC
PacI 1595 TTAATTAA TTAAT^TAA
1783 TTAATTAA
PpuMI 2198 AGGTCCC [GA]G^G[AT]CC[CT]
3830 GGGACCT
RcaI 3627 TCATGA T^CATGA
4031 TCATGA
TaqI 247 TCGA T^CGA
2135 TCGA
TthHB8I 247 TCGA T^CGA
2135 TCGA
XcmI 1050 CCACGGTGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1508 CCAGGGAAAGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AspI AvaII BbsI
BciVI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PflFI PleI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I XhoII
XmnI