##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbvCI BcgI BlnI
BlpI Bpu1102I BsaBI BseAI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MluI MroI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I RsrII SacII
SbfI SexAI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2397 GACGTC GACGT^C
AflII 3889 CTTAAG C^TTAAG
AhdI 1018 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2700 GTGCAC G^TGCAC
AocI 2252 CCTGAGG CC^T[ACGT]AGG
ApaLI 2700 GTGCAC G^TGCAC
AspEI 1018 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2870 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1929 TGATCA T^GATCA
BfrI 3889 CTTAAG C^TTAAG
BglII 3171 AGATCT A^GATCT
BsaWI 881 ACCGGA [AT]^CCGG[AT]
BsiWI 1234 CGTACG C^GTACG
BsmBI 2931 GAGACG CGTCTC(1/5)
BsmI 924 GCATTC GAATGC(1/-1)
BspHI 1926 TCATGA T^CATGA
BspMI 120 GCAGGT ACCTGC(4/8)
BssHII 257 GCGCGC G^CGCGC
BssSI 1000 CTCGTG CACGAG(-5/-1)
Bsu36I 2252 CCTGAGG CC^T[ACGT]AGG
DraIII 4297 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 713 CGGCCG C^GGCCG
Eam1105I 1018 GACGCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 713 CGGCCG C^GGCCG
Eco52I 713 CGGCCG C^GGCCG
Eco81I 2252 CCTGAGG CC^T[ACGT]AGG
FauI 1600 GCGGG CCCGC(4/6)
FbaI 1929 TGATCA T^GATCA
HaeII 340 GGCGCT [GA]GCGC^[CT]
MfeI 703 CAATTG C^AATTG
MunI 703 CAATTG C^AATTG
PflFI 2870 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1066 CAGCTG CAG^CTG
RcaI 1926 TCATGA T^CATGA
SacI 872 GAGCTC GAGCT^C
SalI 1944 GTCGAC G^TCGAC
ScaI 2118 AGTACT AGT^ACT
SfiI 3686 GGCCCATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3291 CCCGGG CCC^GGG
SpeI 3831 ACTAGT A^CTAGT
Tth111I 2870 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3291 CCCGGG C^CCGGG
XmaI 3291 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1955 AAATTT [GA]^AATT[CT]
3862 AAATTT
AcyI 384 GGCGTC G[GA]^CG[CT]C
2397 GACGTC
AfaI 1235 GTAC GT^AC
2119 GTAC
AflIII 1489 ACGTGT A^C[GA][CT]GT
2154 ACACGT
ApoI 1955 AAATTT [GA]^AATT[CT]
3862 AAATTT
Asp700I 727 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1051 GAACTTTTTC
AvaI 73 CTCGGG C^[CT]CG[GA]G
3291 CCCGGG
BamHI 2501 GGATCC G^GATCC
3938 GGATCC
BbrPI 1488 CACGTG CAC^GTG
2155 CACGTG
BciVI 2847 GGATAC GTATCC(6/5)
1868 GTATCC
BsaAI 1488 CACGTG [CT]AC^GT[GA]
2155 CACGTG
BsaHI 384 GGCGTC G[GA]^CG[CT]C
2397 GACGTC
BsaI 2828 GGTCTC GGTCTC(1/5)
2985 GGTCTC
BsoBI 73 CTCGGG C^[CT]CG[GA]G
3291 CCCGGG
EcoNI 2463 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3130 CCTTGCAGAGG
Hin1I 384 GGCGTC G[GA]^CG[CT]C
2397 GACGTC
HincII 1223 GTCAAC GT[CT]^[GA]AC
1944 GTCGAC
HindII 1223 GTCAAC GT[CT]^[GA]AC
1944 GTCGAC
HindIII 3188 AAGCTT A^AGCTT
4197 AAGCTT
MspA1I 1066 CAGCTG C[AC]G^C[GT]G
1078 CCGCTG
NaeI 307 GCCGGC GCC^GGC
708 GCCGGC
NgoMIV 307 GCCGGC G^CCGGC
708 GCCGGC
PmaCI 1488 CACGTG CAC^GTG
2155 CACGTG
PmlI 1488 CACGTG CAC^GTG
2155 CACGTG
PpuMI 1969 GGGTCCC [GA]G^G[AT]CC[CT]
3908 GGGTCCT
PvuI 297 CGATCG CGAT^CG
566 CGATCG
RsaI 1235 GTAC GT^AC
2119 GTAC
TaqI 1536 TCGA T^CGA
1945 TCGA
TthHB8I 1536 TCGA T^CGA
1945 TCGA
XmnI 727 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1051 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PstI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII