##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HapII HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 1496 GAATTC [GA]^AATT[CT]
AflIII 1405 ACATGT A^C[GA][CT]GT
ApoI 1496 GAATTC [GA]^AATT[CT]
Asp700I 1577 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2263 GTGCTC G[AT]GC[AT]^C
BclI 1631 TGATCA T^GATCA
BglII 415 AGATCT A^GATCT
BsaBI 410 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2263 GTGCTC G[AT]GC[AT]^C
BsmBI 2817 GAGACG CGTCTC(1/5)
Bsp1407I 599 TGTACA T^GTACA
BspHI 78 TCATGA T^CATGA
BspLU11I 1405 ACATGT A^CATGT
BspMI 955 ACCTGC ACCTGC(4/8)
BsrBI 2258 GAGCGG CCGCTC(-3/-3)
BsrGI 599 TGTACA T^GTACA
DraI 593 TTTAAA TTT^AAA
DraII 2250 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 2164 CCATGG C^C[GA][CT]GG
EcoNI 492 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2250 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1496 GAATTC G^AATTC
FbaI 1631 TGATCA T^GATCA
HaeII 1925 AGCGCC [GA]GCGC^[CT]
HincII 1196 GTCAAC GT[CT]^[GA]AC
HindII 1196 GTCAAC GT[CT]^[GA]AC
MaeII 1950 ACGT A^CGT
MamI 410 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2164 CCATGG C^CATGG
NheI 701 GCTAGC G^CTAGC
NspI 1405 ACATGT [GA]CATG^[CT]
PpuMI 2250 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 78 TCATGA T^CATGA
SexAI 2365 ACCTGGT A^CC[AT]GGT
SspBI 599 TGTACA T^GTACA
TaqI 1373 TCGA T^CGA
TthHB8I 1373 TCGA T^CGA
XmnI 1577 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 734 GACGCC G[GA]^CG[CT]C
1022 GACGCC
AfaI 600 GTAC GT^AC
2293 GTAC
BanI 2201 GGCACC G^G[CT][GA]CC
2226 GGTGCC
BbsI 401 GAAGAC GAAGAC(2/6)
338 GTCTTC
BfaI 702 CTAG C^TAG
1798 CTAG
BglI 2577 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2607 GCCTCCAAGGC
BpuAI 401 GAAGAC GAAGAC(2/6)
338 GTCTTC
BsaHI 734 GACGCC G[GA]^CG[CT]C
1022 GACGCC
BstXI 460 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
554 CCAACTCGCTGG
DraIII 199 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
295 CACCAAGTG
FauI 2382 GCGGG CCCGC(4/6)
2581 GCGGG
Hin1I 734 GACGCC G[GA]^CG[CT]C
1022 GACGCC
MaeI 702 CTAG C^TAG
1798 CTAG
MslI 866 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1559 CATCCGTGTG
PflMI 1710 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2072 CCAGGTGATGG
RsaI 600 GTAC GT^AC
2293 GTAC
Van91I 1710 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2072 CCAGGTGATGG
XcmI 1555 CCAACATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2068 CCAGCCAGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvCI BbvI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII