################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HapII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 1496 GAATTC [GA]^AATT[CT] AflIII 1405 ACATGT A^C[GA][CT]GT ApoI 1496 GAATTC [GA]^AATT[CT] Asp700I 1577 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 2263 GTGCTC G[AT]GC[AT]^C BclI 1631 TGATCA T^GATCA BglII 415 AGATCT A^GATCT BsaBI 410 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2263 GTGCTC G[AT]GC[AT]^C BsmBI 2817 GAGACG CGTCTC(1/5) Bsp1407I 599 TGTACA T^GTACA BspHI 78 TCATGA T^CATGA BspLU11I 1405 ACATGT A^CATGT BspMI 955 ACCTGC ACCTGC(4/8) BsrBI 2258 GAGCGG CCGCTC(-3/-3) BsrGI 599 TGTACA T^GTACA DraI 593 TTTAAA TTT^AAA DraII 2250 GGGACCC [GA]G^G[ACGT]CC[CT] DsaI 2164 CCATGG C^C[GA][CT]GG EcoNI 492 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 2250 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 1496 GAATTC G^AATTC FbaI 1631 TGATCA T^GATCA HaeII 1925 AGCGCC [GA]GCGC^[CT] HincII 1196 GTCAAC GT[CT]^[GA]AC HindII 1196 GTCAAC GT[CT]^[GA]AC MaeII 1950 ACGT A^CGT MamI 410 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 2164 CCATGG C^CATGG NheI 701 GCTAGC G^CTAGC NspI 1405 ACATGT [GA]CATG^[CT] PpuMI 2250 GGGACCC [GA]G^G[AT]CC[CT] RcaI 78 TCATGA T^CATGA SexAI 2365 ACCTGGT A^CC[AT]GGT SspBI 599 TGTACA T^GTACA TaqI 1373 TCGA T^CGA TthHB8I 1373 TCGA T^CGA XmnI 1577 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 734 GACGCC G[GA]^CG[CT]C 1022 GACGCC AfaI 600 GTAC GT^AC 2293 GTAC BanI 2201 GGCACC G^G[CT][GA]CC 2226 GGTGCC BbsI 401 GAAGAC GAAGAC(2/6) 338 GTCTTC BfaI 702 CTAG C^TAG 1798 CTAG BglI 2577 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2607 GCCTCCAAGGC BpuAI 401 GAAGAC GAAGAC(2/6) 338 GTCTTC BsaHI 734 GACGCC G[GA]^CG[CT]C 1022 GACGCC BstXI 460 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 554 CCAACTCGCTGG DraIII 199 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 295 CACCAAGTG FauI 2382 GCGGG CCCGC(4/6) 2581 GCGGG Hin1I 734 GACGCC G[GA]^CG[CT]C 1022 GACGCC MaeI 702 CTAG C^TAG 1798 CTAG MslI 866 CACGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1559 CATCCGTGTG PflMI 1710 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2072 CCAGGTGATGG RsaI 600 GTAC GT^AC 2293 GTAC Van91I 1710 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2072 CCAGGTGATGG XcmI 1555 CCAACATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2068 CCAGCCAGGTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AluI AlwI AlwNI AvaII BanII BbvCI BbvI BmyI BpmI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII