##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
Asp718I AviII AvrII BbsI BciVI
BfrI BlnI BpuAI BsaBI BsiWI
BsmBI BspDI BspLU11I BssSI Bst1107I
BstZ17I ClaI CpoI DraI DraIII
EcoRI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII ScaI SexAI SnaBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 680 GACGTC GACGT^C
AccIII 2974 TCCGGA T^CCGGA
AcsI 1571 AAATTT [GA]^AATT[CT]
AhdI 45 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2288 GTGCAC G^TGCAC
AocI 2260 CCTCAGG CC^T[ACGT]AGG
ApaLI 2288 GTGCAC G^TGCAC
ApoI 1571 AAATTT [GA]^AATT[CT]
AspEI 45 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1592 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 756 TGGCCA TGG^CCA
BbrPI 3798 CACGTG CAC^GTG
BcgI 2192 GCAATTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2490 TGATCA T^GATCA
BlpI 3675 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3675 GCTCAGC GC^T[ACGT]AGC
BsaI 2812 GGTCTC GGTCTC(1/5)
BseAI 2974 TCCGGA T^CCGGA
BsmI 3383 GCATTC GAATGC(1/-1)
BspEI 2974 TCCGGA T^CCGGA
BspHI 2823 TCATGA T^CATGA
BsrDI 2440 CATTGC GCAATG(2/0)
BstAPI 3383 GCATTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1359 TTCGAA TT^CGAA
BstXI 2418 CCACTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2260 CCTCAGG CC^T[ACGT]AGG
CelII 3675 GCTCAGC GC^T[ACGT]AGC
Eam1105I 45 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2260 CCTCAGG CC^T[ACGT]AGG
EcoRV 1442 GATATC GAT^ATC
EcoT22I 3390 ATGCAT ATGCA^T
FbaI 2490 TGATCA T^GATCA
HincII 2203 GTCGAC GT[CT]^[GA]AC
HindII 2203 GTCGAC GT[CT]^[GA]AC
MluNI 756 TGGCCA TGG^CCA
MroI 2974 TCCGGA T^CCGGA
MscI 756 TGGCCA TGG^CCA
NaeI 3119 GCCGGC GCC^GGC
NgoMIV 3119 GCCGGC G^CCGGC
NsiI 3390 ATGCAT ATGCA^T
NspV 1359 TTCGAA TT^CGAA
PflFI 1592 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2563 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3798 CACGTG CAC^GTG
PmlI 3798 CACGTG CAC^GTG
PpuMI 3173 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 2823 TCATGA T^CATGA
SalI 2203 GTCGAC G^TCGAC
SapI 3788 GCTCTTC GCTCTTC(1/4)
SbfI 937 CCTGCAGG CCTGCA^GG
SfiI 3113 GGCCTCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1359 TTCGAA TT^CGAA
SgrAI 690 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1925 CTTGAG C^T[CT][GA]AG
SpeI 1781 ACTAGT A^CTAGT
Sse8387I 937 CCTGCAGG CCTGCA^GG
SspI 1232 AATATT AAT^ATT
Tth111I 1592 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2563 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2836 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2203 GTCGAC GT^[AC][GT]AC
3453 GTAGAC
AlwNI 2136 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
3870 CAGCGGCTG
AseI 1235 ATTAAT AT^TAAT
2632 ATTAAT
AsnI 1235 ATTAAT AT^TAAT
2632 ATTAAT
Asp700I 1202 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1568 GAAAAATTTC
BbeI 376 GGCGCC GGCGC^C
3036 GGCGCC
BsaAI 1184 TACGTG [CT]AC^GT[GA]
3798 CACGTG
Bsp1407I 2241 TGTACA T^GTACA
4110 TGTACA
BsrFI 691 ACCGGC [GA]^CCGG[CT]
3119 GCCGGC
BsrGI 2241 TGTACA T^GTACA
4110 TGTACA
BstEII 1147 GGTCACC G^GT[ACGT]ACC
3974 GGTAACC
BstPI 1147 GGTCACC G^GT[ACGT]ACC
3974 GGTAACC
Cfr10I 691 ACCGGC [GA]^CCGG[CT]
3119 GCCGGC
DrdI 1466 GACTCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3611 GACAATTTGGTC
EagI 230 CGGCCG C^GGCCG
812 CGGCCG
EclXI 230 CGGCCG C^GGCCG
812 CGGCCG
Eco52I 230 CGGCCG C^GGCCG
812 CGGCCG
EcoO65I 1147 GGTCACC G^GT[ACGT]ACC
3974 GGTAACC
KasI 376 GGCGCC G^GCGCC
3036 GGCGCC
MslI 2419 CACTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3796 CACACGTGTG
NarI 376 GGCGCC GG^CGCC
3036 GGCGCC
NcoI 2235 CCATGG C^CATGG
2272 CCATGG
PshBI 1235 ATTAAT AT^TAAT
2632 ATTAAT
SfoI 376 GGCGCC GGC^GCC
3036 GGCGCC
SphI 3858 GCATGC GCATG^C
3991 GCATGC
SspBI 2241 TGTACA T^GTACA
4110 TGTACA
XcmI 2563 CCAGATCTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3754 CCACTTCTTCCCTGG
XmnI 1202 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1568 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI Aor51HI ApaI AspHI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI