##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BbrPI
BbvCI BfrI BlnI BsaBI BsaWI
BseAI BspEI BspHI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CpoI DraI DrdI
Eam1105I Eco47III EcoO65I FspI HindIII
KpnI MamI MfeI MroI MunI
NcoI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2641 GACGTC GACGT^C
AccI 1267 GTCGAC GT^[AC][GT]AC
AflIII 977 ACGCGT A^C[GA][CT]GT
AocI 2031 CCTAAGG CC^T[ACGT]AGG
Asp700I 1537 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 2135 GCAGTGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 378 GTATCC GTATCC(6/5)
BclI 3116 TGATCA T^GATCA
BglII 2937 AGATCT A^GATCT
BsiWI 941 CGTACG C^GTACG
BspDI 2143 ATCGAT AT^CGAT
BspMI 593 ACCTGC ACCTGC(4/8)
BsrBI 3081 CCGCTC CCGCTC(-3/-3)
Bsu36I 2031 CCTAAGG CC^T[ACGT]AGG
ClaI 2143 ATCGAT AT^CGAT
Eco81I 2031 CCTAAGG CC^T[ACGT]AGG
EcoRV 211 GATATC GAT^ATC
FbaI 3116 TGATCA T^GATCA
FseI 846 GGCCGGCC GGCCGG^CC
HpaI 3733 GTTAAC GTT^AAC
MluI 977 ACGCGT A^CGCGT
PaeR7I 238 CTCGAG C^TCGAG
SalI 1267 GTCGAC G^TCGAC
SapI 172 GAAGAGC GCTCTTC(1/4)
ScaI 1917 AGTACT AGT^ACT
SphI 3592 GCATGC GCATG^C
SspI 3694 AATATT AAT^ATT
XcmI 959 CCACCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 238 CTCGAG C^TCGAG
XmnI 1537 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 338 GTGCAC G^TGCAC
3150 GTGCAC
ApaLI 338 GTGCAC G^TGCAC
3150 GTGCAC
BamHI 133 GGATCC G^GATCC
2387 GGATCC
BbeI 742 GGCGCC GGCGC^C
2479 GGCGCC
BlpI 921 GCTGAGC GC^T[ACGT]AGC
3083 GCTCAGC
Bpu1102I 921 GCTGAGC GC^T[ACGT]AGC
3083 GCTCAGC
BsaAI 939 CACGTA [CT]AC^GT[GA]
2110 TACGTG
BsiEI 845 CGGCCG CG[GA][CT]^CG
2048 CGGCCG
BsmBI 2213 CGTCTC CGTCTC(1/5)
3854 GAGACG
Bsp1407I 4007 TGTACA T^GTACA
4061 TGTACA
BsrGI 4007 TGTACA T^GTACA
4061 TGTACA
BssSI 1239 CACGAG CACGAG(-5/-1)
2566 CTCGTG
CelII 921 GCTGAGC GC^T[ACGT]AGC
3083 GCTCAGC
EagI 845 CGGCCG C^GGCCG
2048 CGGCCG
EclXI 845 CGGCCG C^GGCCG
2048 CGGCCG
Eco52I 845 CGGCCG C^GGCCG
2048 CGGCCG
EcoRI 3252 GAATTC G^AATTC
3360 GAATTC
EcoT22I 3594 ATGCAT ATGCA^T
4038 ATGCAT
KasI 742 GGCGCC G^GCGCC
2479 GGCGCC
KspI 553 CCGCGG CCGC^GG
1322 CCGCGG
NarI 742 GGCGCC GG^CGCC
2479 GGCGCC
NsiI 3594 ATGCAT ATGCA^T
4038 ATGCAT
PflMI 402 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1659 CCAGCTCCTGG
PshAI 1692 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2757 GACAGCTGTC
SacII 553 CCGCGG CCGC^GG
1322 CCGCGG
SfoI 742 GGCGCC GGC^GCC
2479 GGCGCC
SmlI 238 CTCGAG C^T[CT][GA]AG
3448 CTCAAG
SspBI 4007 TGTACA T^GTACA
4061 TGTACA
StuI 2553 AGGCCT AGG^CCT
2813 AGGCCT
Van91I 402 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1659 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII