################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BbvCI BfrI BlnI BsaBI BsaWI BseAI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DraI DrdI Eam1105I Eco47III EcoO65I FspI HindIII KpnI MamI MfeI MroI MunI NcoI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### AatII 2641 GACGTC GACGT^C AccI 1267 GTCGAC GT^[AC][GT]AC AflIII 977 ACGCGT A^C[GA][CT]GT AocI 2031 CCTAAGG CC^T[ACGT]AGG Asp700I 1537 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 2135 GCAGTGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 378 GTATCC GTATCC(6/5) BclI 3116 TGATCA T^GATCA BglII 2937 AGATCT A^GATCT BsiWI 941 CGTACG C^GTACG BspDI 2143 ATCGAT AT^CGAT BspMI 593 ACCTGC ACCTGC(4/8) BsrBI 3081 CCGCTC CCGCTC(-3/-3) Bsu36I 2031 CCTAAGG CC^T[ACGT]AGG ClaI 2143 ATCGAT AT^CGAT Eco81I 2031 CCTAAGG CC^T[ACGT]AGG EcoRV 211 GATATC GAT^ATC FbaI 3116 TGATCA T^GATCA FseI 846 GGCCGGCC GGCCGG^CC HpaI 3733 GTTAAC GTT^AAC MluI 977 ACGCGT A^CGCGT PaeR7I 238 CTCGAG C^TCGAG SalI 1267 GTCGAC G^TCGAC SapI 172 GAAGAGC GCTCTTC(1/4) ScaI 1917 AGTACT AGT^ACT SphI 3592 GCATGC GCATG^C SspI 3694 AATATT AAT^ATT XcmI 959 CCACCAAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 238 CTCGAG C^TCGAG XmnI 1537 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Alw44I 338 GTGCAC G^TGCAC 3150 GTGCAC ApaLI 338 GTGCAC G^TGCAC 3150 GTGCAC BamHI 133 GGATCC G^GATCC 2387 GGATCC BbeI 742 GGCGCC GGCGC^C 2479 GGCGCC BlpI 921 GCTGAGC GC^T[ACGT]AGC 3083 GCTCAGC Bpu1102I 921 GCTGAGC GC^T[ACGT]AGC 3083 GCTCAGC BsaAI 939 CACGTA [CT]AC^GT[GA] 2110 TACGTG BsiEI 845 CGGCCG CG[GA][CT]^CG 2048 CGGCCG BsmBI 2213 CGTCTC CGTCTC(1/5) 3854 GAGACG Bsp1407I 4007 TGTACA T^GTACA 4061 TGTACA BsrGI 4007 TGTACA T^GTACA 4061 TGTACA BssSI 1239 CACGAG CACGAG(-5/-1) 2566 CTCGTG CelII 921 GCTGAGC GC^T[ACGT]AGC 3083 GCTCAGC EagI 845 CGGCCG C^GGCCG 2048 CGGCCG EclXI 845 CGGCCG C^GGCCG 2048 CGGCCG Eco52I 845 CGGCCG C^GGCCG 2048 CGGCCG EcoRI 3252 GAATTC G^AATTC 3360 GAATTC EcoT22I 3594 ATGCAT ATGCA^T 4038 ATGCAT KasI 742 GGCGCC G^GCGCC 2479 GGCGCC KspI 553 CCGCGG CCGC^GG 1322 CCGCGG NarI 742 GGCGCC GG^CGCC 2479 GGCGCC NsiI 3594 ATGCAT ATGCA^T 4038 ATGCAT PflMI 402 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1659 CCAGCTCCTGG PshAI 1692 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2757 GACAGCTGTC SacII 553 CCGCGG CCGC^GG 1322 CCGCGG SfoI 742 GGCGCC GGC^GCC 2479 GGCGCC SmlI 238 CTCGAG C^T[CT][GA]AG 3448 CTCAAG SspBI 4007 TGTACA T^GTACA 4061 TGTACA StuI 2553 AGGCCT AGG^CCT 2813 AGGCCT Van91I 402 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1659 CCAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII