##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I AocI ApaI ApaLI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BciVI
BfrI BglII BlnI BsaBI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssSI Bst1107I BstBI BstZ17I Bsu36I
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MscI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 3607 AACGTT AA^CGTT
AlwNI 1489 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1746 AGCGCT AGC^GCT
AscI 106 GGCGCGCC GG^CGCGCC
AseI 2925 ATTAAT AT^TAAT
AsnI 2925 ATTAAT AT^TAAT
Asp700I 268 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 568 GGCACC G^G[CT][GA]CC
BbrPI 3103 CACGTG CAC^GTG
BclI 4054 TGATCA T^GATCA
BglI 14 GCCACGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1675 GAGACC GGTCTC(1/5)
BsmI 2523 GAATGC GAATGC(1/-1)
BspHI 1112 TCATGA T^CATGA
BspLU11I 699 ACATGT A^CATGT
BssHII 107 GCGCGC G^CGCGC
BstEII 1247 GGTCACC G^GT[ACGT]ACC
BstPI 1247 GGTCACC G^GT[ACGT]ACC
BstXI 1641 CCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 127 AGATCC [GA]^GATC[CT]
DraII 3056 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1250 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1746 AGCGCT AGC^GCT
EcoO109I 3056 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1247 GGTCACC G^GT[ACGT]ACC
EcoRI 4121 GAATTC G^AATTC
EcoRV 3234 GATATC GAT^ATC
FbaI 4054 TGATCA T^GATCA
HaeII 1746 AGCGCT [GA]GCGC^[CT]
MflI 127 AGATCC [GA]^GATC[CT]
NheI 3723 GCTAGC G^CTAGC
NspI 699 ACATGT [GA]CATG^[CT]
PmaCI 3103 CACGTG CAC^GTG
PmlI 3103 CACGTG CAC^GTG
PpuMI 3056 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2925 ATTAAT AT^TAAT
Psp1406I 3607 AACGTT AA^CGTT
RcaI 1112 TCATGA T^CATGA
ScaI 2342 AGTACT AGT^ACT
StuI 3890 AGGCCT AGG^CCT
XcmI 665 CCATTCTATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 127 AGATCC [GA]^GATC[CT]
XmnI 268 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 130 TCCGGA T^CCGGA
3436 TCCGGA
AcyI 658 GACGCC G[GA]^CG[CT]C
1852 GGCGTC
AvaI 144 CCCGAG C^[CT]CG[GA]G
1355 CCCGAG
BcnI 745 CCCGG CC^[GC]GG
2050 CCCGG
BpmI 114 CTGGAG CTGGAG(16/14)
1426 CTGGAG
BsaHI 658 GACGCC G[GA]^CG[CT]C
1852 GGCGTC
BsaWI 130 TCCGGA [AT]^CCGG[AT]
3436 TCCGGA
BseAI 130 TCCGGA T^CCGGA
3436 TCCGGA
BsgI 1828 CTGCAC GTGCAG(16/14)
3106 GTGCAG
BsoBI 144 CCCGAG C^[CT]CG[GA]G
1355 CCCGAG
BspEI 130 TCCGGA T^CCGGA
3436 TCCGGA
BspMI 2500 ACCTGC ACCTGC(4/8)
1599 GCAGGT
BstAPI 617 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1506 GCAAGTTGTGC
EcoNI 675 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1425 CCTGGAGGAGG
Hin1I 658 GACGCC G[GA]^CG[CT]C
1852 GGCGTC
MfeI 449 CAATTG C^AATTG
2477 CAATTG
MroI 130 TCCGGA T^CCGGA
3436 TCCGGA
MunI 449 CAATTG C^AATTG
2477 CAATTG
NciI 745 CCCGG CC^[GC]GG
2050 CCCGG
PflMI 1419 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2061 CCATTTTCTGG
PstI 1597 CTGCAG CTGCA^G
1926 CTGCAG
PvuII 761 CAGCTG CAG^CTG
1492 CAGCTG
SapI 1545 GCTCTTC GCTCTTC(1/4)
2351 GCTCTTC
TaqI 1637 TCGA T^CGA
2007 TCGA
TthHB8I 1637 TCGA T^CGA
2007 TCGA
Van91I 1419 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2061 CCATTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanII BbsI BbvI BfaI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI