################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AvrII BamHI BbeI BbrPI BlnI BsaAI BsaWI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoT22I FseI HgaI HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI TaqI TthHB8I XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 4081 GACGTC GACGT^C AccI 4361 GTCTAC GT^[AC][GT]AC AcyI 4081 GACGTC G[GA]^CG[CT]C AflII 4061 CTTAAG C^TTAAG AspI 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 3024 GAAGAC GAAGAC(2/6) BbvCI 2272 GCTGAGG CCTCAGC(-5/-2) BclI 4317 TGATCA T^GATCA BfrI 4061 CTTAAG C^TTAAG BpuAI 3024 GAAGAC GAAGAC(2/6) BsaHI 4081 GACGTC G[GA]^CG[CT]C BsiEI 3781 CGGCCG CG[GA][CT]^CG BsmI 4426 GCATTC GAATGC(1/-1) Bsp1407I 3852 TGTACA T^GTACA BspHI 1068 TCATGA T^CATGA BsrDI 3637 CATTGC GCAATG(2/0) BsrFI 4288 GCCGGC [GA]^CCGG[CT] BsrGI 3852 TGTACA T^GTACA Cfr10I 4288 GCCGGC [GA]^CCGG[CT] DrdI 4204 GACCTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3781 CGGCCG C^GGCCG EclXI 3781 CGGCCG C^GGCCG Eco52I 3781 CGGCCG C^GGCCG EcoRV 998 GATATC GAT^ATC FbaI 4317 TGATCA T^GATCA HaeII 603 AGCGCC [GA]GCGC^[CT] Hin1I 4081 GACGTC G[GA]^CG[CT]C HincII 4187 GTCAAC GT[CT]^[GA]AC HindII 4187 GTCAAC GT[CT]^[GA]AC NaeI 4288 GCCGGC GCC^GGC NgoMIV 4288 GCCGGC G^CCGGC PflFI 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2199 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 2541 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1068 TCATGA T^CATGA SacI 3629 GAGCTC GAGCT^C SapI 4442 GAAGAGC GCTCTTC(1/4) SmaI 3660 CCCGGG CCC^GGG SspBI 3852 TGTACA T^GTACA Tth111I 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2199 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3660 CCCGGG C^CCGGG XmaI 3660 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 449 GAATTC [GA]^AATT[CT] 2287 GAATTC ApaI 1551 GGGCCC GGGCC^C 3256 GGGCCC ApoI 449 GAATTC [GA]^AATT[CT] 2287 GAATTC Asp700I 2695 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3303 GAAGGGCTTC AvaI 3489 CCCGAG C^[CT]CG[GA]G 3660 CCCGGG AviII 1643 TGCGCA TGC^GCA 4227 TGCGCA BcgI 3491 CGAGATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4350 GCAACCTCTTCG BciVI 842 GGATAC GTATCC(6/5) 4031 GGATAC BglI 749 GCCCGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3340 GCCTGGGTGGC BglII 2447 AGATCT A^GATCT 3493 AGATCT BlpI 2085 GCTGAGC GC^T[ACGT]AGC 3124 GCTGAGC Bpu1102I 2085 GCTGAGC GC^T[ACGT]AGC 3124 GCTGAGC BsaBI 516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 994 GATAGATATC BsaI 1453 GAGACC GGTCTC(1/5) 4202 GAGACC BsoBI 3489 CCCGAG C^[CT]CG[GA]G 3660 CCCGGG BspLU11I 814 ACATGT A^CATGT 4209 ACATGT BsrBI 4465 CCGCTC CCGCTC(-3/-3) 3622 GAGCGG BssSI 1450 CACGAG CACGAG(-5/-1) 3872 CACGAG BstAPI 1646 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3506 GCAGCCAGTGC CelII 2085 GCTGAGC GC^T[ACGT]AGC 3124 GCTGAGC DraIII 249 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1888 CACTCTGTG EcoRI 449 GAATTC G^AATTC 2287 GAATTC FspI 1643 TGCGCA TGC^GCA 4227 TGCGCA MamI 516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 994 GATAGATATC NcoI 1815 CCATGG C^CATGG 4110 CCATGG SmlI 3893 CTCAAG C^T[CT][GA]AG 4061 CTTAAG SspI 398 AATATT AAT^ATT 2687 AATATT XbaI 365 TCTAGA T^CTAGA 2619 TCTAGA XcmI 1773 CCAGAATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4110 CCATGGATCTGATGG XmnI 2695 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3303 GAAGGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### Acc65I AciI AfaI AflIII AluI AlwI AlwNI AocI Asp718I AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII