##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AvrII BamHI BbeI
BbrPI BlnI BsaAI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoT22I
FseI HgaI HindIII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI TaqI TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 4081 GACGTC GACGT^C
AccI 4361 GTCTAC GT^[AC][GT]AC
AcyI 4081 GACGTC G[GA]^CG[CT]C
AflII 4061 CTTAAG C^TTAAG
AspI 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 3024 GAAGAC GAAGAC(2/6)
BbvCI 2272 GCTGAGG CCTCAGC(-5/-2)
BclI 4317 TGATCA T^GATCA
BfrI 4061 CTTAAG C^TTAAG
BpuAI 3024 GAAGAC GAAGAC(2/6)
BsaHI 4081 GACGTC G[GA]^CG[CT]C
BsiEI 3781 CGGCCG CG[GA][CT]^CG
BsmI 4426 GCATTC GAATGC(1/-1)
Bsp1407I 3852 TGTACA T^GTACA
BspHI 1068 TCATGA T^CATGA
BsrDI 3637 CATTGC GCAATG(2/0)
BsrFI 4288 GCCGGC [GA]^CCGG[CT]
BsrGI 3852 TGTACA T^GTACA
Cfr10I 4288 GCCGGC [GA]^CCGG[CT]
DrdI 4204 GACCTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3781 CGGCCG C^GGCCG
EclXI 3781 CGGCCG C^GGCCG
Eco52I 3781 CGGCCG C^GGCCG
EcoRV 998 GATATC GAT^ATC
FbaI 4317 TGATCA T^GATCA
HaeII 603 AGCGCC [GA]GCGC^[CT]
Hin1I 4081 GACGTC G[GA]^CG[CT]C
HincII 4187 GTCAAC GT[CT]^[GA]AC
HindII 4187 GTCAAC GT[CT]^[GA]AC
NaeI 4288 GCCGGC GCC^GGC
NgoMIV 4288 GCCGGC G^CCGGC
PflFI 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2199 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2541 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1068 TCATGA T^CATGA
SacI 3629 GAGCTC GAGCT^C
SapI 4442 GAAGAGC GCTCTTC(1/4)
SmaI 3660 CCCGGG CCC^GGG
SspBI 3852 TGTACA T^GTACA
Tth111I 705 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2199 CCAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3660 CCCGGG C^CCGGG
XmaI 3660 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 449 GAATTC [GA]^AATT[CT]
2287 GAATTC
ApaI 1551 GGGCCC GGGCC^C
3256 GGGCCC
ApoI 449 GAATTC [GA]^AATT[CT]
2287 GAATTC
Asp700I 2695 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3303 GAAGGGCTTC
AvaI 3489 CCCGAG C^[CT]CG[GA]G
3660 CCCGGG
AviII 1643 TGCGCA TGC^GCA
4227 TGCGCA
BcgI 3491 CGAGATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4350 GCAACCTCTTCG
BciVI 842 GGATAC GTATCC(6/5)
4031 GGATAC
BglI 749 GCCCGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3340 GCCTGGGTGGC
BglII 2447 AGATCT A^GATCT
3493 AGATCT
BlpI 2085 GCTGAGC GC^T[ACGT]AGC
3124 GCTGAGC
Bpu1102I 2085 GCTGAGC GC^T[ACGT]AGC
3124 GCTGAGC
BsaBI 516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATAGATATC
BsaI 1453 GAGACC GGTCTC(1/5)
4202 GAGACC
BsoBI 3489 CCCGAG C^[CT]CG[GA]G
3660 CCCGGG
BspLU11I 814 ACATGT A^CATGT
4209 ACATGT
BsrBI 4465 CCGCTC CCGCTC(-3/-3)
3622 GAGCGG
BssSI 1450 CACGAG CACGAG(-5/-1)
3872 CACGAG
BstAPI 1646 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3506 GCAGCCAGTGC
CelII 2085 GCTGAGC GC^T[ACGT]AGC
3124 GCTGAGC
DraIII 249 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1888 CACTCTGTG
EcoRI 449 GAATTC G^AATTC
2287 GAATTC
FspI 1643 TGCGCA TGC^GCA
4227 TGCGCA
MamI 516 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATAGATATC
NcoI 1815 CCATGG C^CATGG
4110 CCATGG
SmlI 3893 CTCAAG C^T[CT][GA]AG
4061 CTTAAG
SspI 398 AATATT AAT^ATT
2687 AATATT
XbaI 365 TCTAGA T^CTAGA
2619 TCTAGA
XcmI 1773 CCAGAATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4110 CCATGGATCTGATGG
XmnI 2695 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3303 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
Acc65I AciI AfaI AflIII AluI
AlwI AlwNI AocI Asp718I AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII