##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BglI
BlnI BmyI BsaAI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspDI BspEI BspHI BsrBI
BsrFI BssSI BstBI BstXI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV FauI FseI FspI
HaeII HgaI KasI KpnI KspI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1113 GACGTC GACGT^C
AccI 1697 GTATAC GT^[AC][GT]AC
AccII 893 CGCG CG^CG
AcyI 1113 GACGTC G[GA]^CG[CT]C
AocI 2142 CCTTAGG CC^T[ACGT]AGG
AspI 3599 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 828 CTCGAG C^[CT]CG[GA]G
BclI 2695 TGATCA T^GATCA
BpmI 1581 CTCCAG CTGGAG(16/14)
BsaBI 1358 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1113 GACGTC G[GA]^CG[CT]C
BseRI 669 CTCCTC GAGGAG(10/8)
BsgI 230 GTGCAG GTGCAG(16/14)
BsmFI 27 GTCCC GGGAC(10/14)
BsoBI 828 CTCGAG C^[CT]CG[GA]G
Bsp1407I 504 TGTACA T^GTACA
BsrGI 504 TGTACA T^GTACA
BssHII 892 GCGCGC G^CGCGC
Bst1107I 1697 GTATAC GTA^TAC
BstAPI 3173 GCACAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3690 GGTAACC G^GT[ACGT]ACC
BstPI 3690 GGTAACC G^GT[ACGT]ACC
BstUI 893 CGCG CG^CG
BstZ17I 1697 GTATAC GTA^TAC
Bsu36I 2142 CCTTAGG CC^T[ACGT]AGG
DraII 1168 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 2142 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1168 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3690 GGTAACC G^GT[ACGT]ACC
EcoRI 1338 GAATTC G^AATTC
EcoT22I 3615 ATGCAT ATGCA^T
FbaI 2695 TGATCA T^GATCA
Hin1I 1113 GACGTC G[GA]^CG[CT]C
HpaI 365 GTTAAC GTT^AAC
MamI 1358 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1494 CAATTG C^AATTG
MunI 1494 CAATTG C^AATTG
MvnI 893 CGCG CG^CG
NsiI 3615 ATGCAT ATGCA^T
PacI 2151 TTAATTAA TTAAT^TAA
PaeR7I 828 CTCGAG C^TCGAG
PflFI 3599 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1168 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 901 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3018 AGTACT AGT^ACT
SexAI 3712 ACCTGGT A^CC[AT]GGT
SpeI 2552 ACTAGT A^CTAGT
SspBI 504 TGTACA T^GTACA
StuI 3373 AGGCCT AGG^CCT
Tth111I 3599 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 828 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 2634 CTTAAG C^TTAAG
2968 CTTAAG
AlwNI 3225 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3902 CAGATACTG
Asp700I 650 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1521 GAACATCTTC
BalI 1012 TGGCCA TGG^CCA
3608 TGGCCA
BciVI 171 GGATAC GTATCC(6/5)
2432 GGATAC
BcnI 30 CCCGG CC^[GC]GG
3590 CCGGG
BfrI 2634 CTTAAG C^TTAAG
2968 CTTAAG
BlpI 287 GCTCAGC GC^T[ACGT]AGC
3569 GCTAAGC
Bpu1102I 287 GCTCAGC GC^T[ACGT]AGC
3569 GCTAAGC
BsmAI 1351 GTCTC GTCTC(1/5)
1852 GTCTC
BspLU11I 3336 ACATGT A^CATGT
3725 ACATGT
CelII 287 GCTCAGC GC^T[ACGT]AGC
3569 GCTAAGC
EaeI 1012 TGGCCA [CT]^GGCC[GA]
3608 TGGCCA
EcoT14I 141 CCAAGG C^C[AT][AT]GG
1015 CCAAGG
HincII 365 GTTAAC GT[CT]^[GA]AC
3332 GTCAAC
HindII 365 GTTAAC GT[CT]^[GA]AC
3332 GTCAAC
MluNI 1012 TGGCCA TGG^CCA
3608 TGGCCA
MscI 1012 TGGCCA TGG^CCA
3608 TGGCCA
MslI 2204 CATAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3461 CATACTAATG
MspA1I 233 CAGCTG C[AC]G^C[GT]G
1533 CAGCTG
NciI 30 CCCGG CC^[GC]GG
3590 CCGGG
NdeI 809 CATATG CA^TATG
1792 CATATG
PflMI 1004 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCAAAGCCTGG
PvuII 233 CAGCTG CAG^CTG
1533 CAGCTG
StyI 141 CCAAGG C^C[AT][AT]GG
1015 CCAAGG
Tsp45I 1423 GTCAC ^GT[GC]AC
3994 GTGAC
Van91I 1004 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1295 CCAAAGCCTGG
XbaI 2127 TCTAGA T^CTAGA
2670 TCTAGA
XmnI 650 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1521 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BglII BpuAI BsaJI BsiYI
BslI BsmI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII