##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AlwNI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AviII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FseI FspI HapII HgaI
Hin1I HpaI HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MspI MvnI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AclI 2756 AACGTT AA^CGTT
Aor51HI 2282 AGCGCT AGC^GCT
AvaI 1901 CCCGAG C^[CT]CG[GA]G
AvaII 3233 GGACC G^G[AT]CC
BamHI 97 GGATCC G^GATCC
BglI 1118 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 395 AGATCT A^GATCT
BsmI 879 GAATGC GAATGC(1/-1)
BsoBI 1901 CCCGAG C^[CT]CG[GA]G
BspMI 2843 ACCTGC ACCTGC(4/8)
Eco47III 2282 AGCGCT AGC^GCT
FauI 3360 GCGGG CCCGC(4/6)
HaeII 2282 AGCGCT [GA]GCGC^[CT]
NcoI 931 CCATGG C^CATGG
NdeI 2892 CATATG CA^TATG
NheI 1539 GCTAGC G^CTAGC
PflMI 571 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2863 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2756 AACGTT AA^CGTT
ScaI 3673 AGTACT AGT^ACT
SmlI 1217 CTCAAG C^T[CT][GA]AG
SpeI 2457 ACTAGT A^CTAGT
Van91I 571 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 244 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 335 GTATAC GT^[AC][GT]AC
2406 GTATAC
AflIII 574 ACATGT A^C[GA][CT]GT
3160 ACATGT
Alw44I 1985 GTGCAC G^TGCAC
3283 GTGCAC
ApaLI 1985 GTGCAC G^TGCAC
3283 GTGCAC
AspHI 1985 GTGCAC G[AT]GC[AT]^C
3283 GTGCAC
AvrII 543 CCTAGG C^CTAGG
3237 CCTAGG
BclI 306 TGATCA T^GATCA
678 TGATCA
BlnI 543 CCTAGG C^CTAGG
3237 CCTAGG
BmyI 1985 GTGCAC G[AGT]GC[ACT]^C
3283 GTGCAC
BpmI 2175 CTCCAG CTGGAG(16/14)
2246 CTGGAG
BsaI 1662 GGTCTC GGTCTC(1/5)
3266 GGTCTC
BseRI 1436 CTCCTC GAGGAG(10/8)
766 GAGGAG
BsiHKAI 1985 GTGCAC G[AT]GC[AT]^C
3283 GTGCAC
Bsp1286I 1985 GTGCAC G[AGT]GC[ACT]^C
3283 GTGCAC
BspLU11I 574 ACATGT A^CATGT
3160 ACATGT
Bst1107I 335 GTATAC GTA^TAC
2406 GTATAC
BstXI 340 CCAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
909 CCAAGTAGATGG
BstZ17I 335 GTATAC GTA^TAC
2406 GTATAC
CfoI 2283 GCGC GCG^C
3222 GCGC
Cfr13I 2272 GGCCC G^G[ACGT]CC
3233 GGACC
DsaI 931 CCATGG C^C[GA][CT]GG
1748 CCGTGG
EcoNI 1582 CCTATGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3208 CCTAATACAGG
EcoT22I 2581 ATGCAT ATGCA^T
3134 ATGCAT
FbaI 306 TGATCA T^GATCA
678 TGATCA
HhaI 2283 GCGC GCG^C
3222 GCGC
HinP1I 2283 GCGC G^CGC
3222 GCGC
HindIII 781 AAGCTT A^AGCTT
1351 AAGCTT
NsiI 2581 ATGCAT ATGCA^T
3134 ATGCAT
Sau96I 2272 GGCCC G^G[ACGT]CC
3233 GGACC
SexAI 658 ACCTGGT A^CC[AT]GGT
3191 ACCTGGT
SfaNI 1801 GCATC GCATC(5/9)
2583 GCATC
SspI 145 AATATT AAT^ATT
593 AATATT
Tsp45I 1933 GTGAC ^GT[GC]AC
1970 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MunI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI ScrFI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII
XmnI