##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvrII BanII BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BsaI BsiEI BsiWI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI HpaI KpnI
KspI MfeI MluI MunI MvnI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 505 TCCGGA T^CCGGA
AcyI 470 GGCGCC G[GA]^CG[CT]C
AflIII 3393 ACGTGT A^C[GA][CT]GT
AseI 2382 ATTAAT AT^TAAT
AsnI 2382 ATTAAT AT^TAAT
Asp700I 1081 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1116 CTCGGG C^[CT]CG[GA]G
BanI 470 GGCGCC G^G[CT][GA]CC
BbeI 470 GGCGCC GGCGC^C
BciVI 3874 GGATAC GTATCC(6/5)
BglII 2879 AGATCT A^GATCT
BsaAI 1628 TACGTG [CT]AC^GT[GA]
BsaBI 2823 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 470 GGCGCC G[GA]^CG[CT]C
BseAI 505 TCCGGA T^CCGGA
BsmBI 366 GAGACG CGTCTC(1/5)
BsoBI 1116 CTCGGG C^[CT]CG[GA]G
BspEI 505 TCCGGA T^CCGGA
BssSI 733 CACGAG CACGAG(-5/-1)
BstAPI 4294 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3014 GGTCACC G^GT[ACGT]ACC
BstPI 3014 GGTCACC G^GT[ACGT]ACC
DraII 3088 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1905 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 352 CCACGG C^C[GA][CT]GG
EcoNI 474 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3088 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3014 GGTCACC G^GT[ACGT]ACC
FauI 376 GCGGG CCCGC(4/6)
HgaI 368 GACGC GACGC(5/10)
Hin1I 470 GGCGCC G[GA]^CG[CT]C
HincII 3122 GTCAAC GT[CT]^[GA]AC
HindII 3122 GTCAAC GT[CT]^[GA]AC
KasI 470 GGCGCC G^GCGCC
MamI 2823 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 505 TCCGGA T^CCGGA
MspA1I 1885 CAGCTG C[AC]G^C[GT]G
NarI 470 GGCGCC GG^CGCC
NdeI 1394 CATATG CA^TATG
PflMI 3093 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2382 ATTAAT AT^TAAT
PvuII 1885 CAGCTG CAG^CTG
SapI 396 GCTCTTC GCTCTTC(1/4)
ScaI 4362 AGTACT AGT^ACT
SfoI 470 GGCGCC GGC^GCC
SphI 3175 GCATGC GCATG^C
Van91I 3093 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1081 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 713 CCGC CCGC(-3/-1)
376 GCGG
AlwNI 1134 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3261 CAGGTTCTG
AocI 687 CCTGAGG CC^T[ACGT]AGG
4080 CCTAAGG
AspHI 1502 GAGCAC G[AT]GC[AT]^C
3164 GAGCAC
AvaII 360 GGACC G^G[AT]CC
873 GGTCC
AviII 86 TGCGCA TGC^GCA
540 TGCGCA
BamHI 718 GGATCC G^GATCC
4085 GGATCC
BlpI 381 GCTGAGC GC^T[ACGT]AGC
3179 GCTTAGC
Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC
3179 GCTTAGC
BsaWI 362 ACCGGA [AT]^CCGG[AT]
505 TCCGGA
BsgI 730 CTGCAC GTGCAG(16/14)
649 GTGCAG
BsiHKAI 1502 GAGCAC G[AT]GC[AT]^C
3164 GAGCAC
BsmAI 366 GAGAC GTCTC(1/5)
415 GAGAC
BsmI 2146 GAATGC GAATGC(1/-1)
2873 GCATTC
Bsu36I 687 CCTGAGG CC^T[ACGT]AGG
4080 CCTAAGG
CelII 381 GCTGAGC GC^T[ACGT]AGC
3179 GCTTAGC
DrdI 1756 GACACAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1783 GACTGGCGAGTC
Eco81I 687 CCTGAGG CC^T[ACGT]AGG
4080 CCTAAGG
EcoRI 2098 GAATTC G^AATTC
2283 GAATTC
EcoT22I 985 ATGCAT ATGCA^T
1295 ATGCAT
FspI 86 TGCGCA TGC^GCA
540 TGCGCA
HapII 363 CCGG C^CGG
506 CCGG
HindIII 1316 AAGCTT A^AGCTT
3739 AAGCTT
HpaII 363 CCGG C^CGG
506 CCGG
MslI 593 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1902 CACCACTGTG
MspI 363 CCGG C^CGG
506 CCGG
NsiI 985 ATGCAT ATGCA^T
1295 ATGCAT
PstI 2832 CTGCAG CTGCA^G
3382 CTGCAG
SpeI 2200 ACTAGT A^CTAGT
2798 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BalI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII