##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
BamHI BbrPI BcgI BsaAI BsaBI
BsaI BsiEI BsiWI BspDI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FseI FspI KspI MamI MfeI
MluI MunI MvnI NaeI NgoMIV
NheI NotI NruI NspV PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 406 GGTACC G^GTACC
AccIII 3755 TCCGGA T^CCGGA
AcyI 1596 GGCGCC G[GA]^CG[CT]C
Asp700I 4624 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 406 GGTACC G^GTACC
AspHI 4560 GTGCTC G[AT]GC[AT]^C
BbeI 1596 GGCGCC GGCGC^C
BclI 250 TGATCA T^GATCA
BglI 374 GCCATTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1567 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1567 GCTCAGC GC^T[ACGT]AGC
BsaHI 1596 GGCGCC G[GA]^CG[CT]C
BsaWI 3755 TCCGGA [AT]^CCGG[AT]
BseAI 3755 TCCGGA T^CCGGA
BsiHKAI 4560 GTGCTC G[AT]GC[AT]^C
BsmBI 4408 CGTCTC CGTCTC(1/5)
BsmI 3577 GAATGC GAATGC(1/-1)
BspEI 3755 TCCGGA T^CCGGA
BstXI 3678 CCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1567 GCTCAGC GC^T[ACGT]AGC
CfoI 1597 GCGC GCG^C
DraII 472 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 4427 GACCTTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 472 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2734 ATGCAT ATGCA^T
FauI 4349 CCCGC CCCGC(4/6)
FbaI 250 TGATCA T^GATCA
HaeII 1596 GGCGCC [GA]GCGC^[CT]
HhaI 1597 GCGC GCG^C
Hin1I 1596 GGCGCC G[GA]^CG[CT]C
HinP1I 1597 GCGC G^CGC
KasI 1596 GGCGCC G^GCGCC
KpnI 406 GGTACC GGTAC^C
MroI 3755 TCCGGA T^CCGGA
MslI 3433 CACACTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 4172 CAGCTG C[AC]G^C[GT]G
NarI 1596 GGCGCC GG^CGCC
NcoI 2056 CCATGG C^CATGG
NdeI 948 CATATG CA^TATG
NsiI 2734 ATGCAT ATGCA^T
PacI 4156 TTAATTAA TTAAT^TAA
PflMI 4634 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 472 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 4172 CAGCTG CAG^CTG
SfoI 1596 GGCGCC GGC^GCC
SmaI 1648 CCCGGG CCC^GGG
SphI 3828 GCATGC GCATG^C
Van91I 4634 CCATACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 411 CCATTCCAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1648 CCCGGG C^CCGGG
XmaI 1648 CCCGGG C^CCGGG
XmnI 4624 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 4350 CCGC CCGC(-3/-1)
41 GCGG
AflII 1953 CTTAAG C^TTAAG
2344 CTTAAG
AflIII 634 ACATGT A^C[GA][CT]GT
1329 ACATGT
AlwNI 653 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
4172 CAGCTGCTG
AvrII 1544 CCTAGG C^CTAGG
2606 CCTAGG
BanII 2225 GGGCTC G[GA]GC[CT]^C
3812 GGGCTC
BbvCI 1624 GCTGAGG CCTCAGC(-5/-2)
2812 GCTGAGG
BfrI 1953 CTTAAG C^TTAAG
2344 CTTAAG
BglII 223 AGATCT A^GATCT
2621 AGATCT
BlnI 1544 CCTAGG C^CTAGG
2606 CCTAGG
BspLU11I 634 ACATGT A^CATGT
1329 ACATGT
BspMI 838 ACCTGC ACCTGC(4/8)
1064 ACCTGC
DsaI 522 CCGTGG C^C[GA][CT]GG
2056 CCATGG
EarI 3888 CTCTTC CTCTTC(1/4)
1021 GAAGAG
HgaI 2806 GACGC GACGC(5/10)
4125 GACGC
HindIII 3182 AAGCTT A^AGCTT
4686 AAGCTT
HpaI 1309 GTTAAC GTT^AAC
3198 GTTAAC
Ksp632I 3888 CTCTTC CTCTTC(1/4)
1021 GAAGAG
ScaI 2992 AGTACT AGT^ACT
3007 AGTACT
TaqI 506 TCGA T^CGA
1680 TCGA
TthHB8I 506 TCGA T^CGA
1680 TCGA
XbaI 226 TCTAGA T^CTAGA
3610 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI AvaI AvaII BalI
BanI BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII