##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI BanII BbeI
BbrPI BbvCI BcgI BcnI BfrI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmFI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FauI FseI HapII HpaII KasI
KspI MamI MluI MroI MspI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 2106 CGCG CG^CG
AcyI 371 GGCGTC G[GA]^CG[CT]C
AlwNI 2161 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 4073 ATTAAT AT^TAAT
AsnI 4073 ATTAAT AT^TAAT
AspI 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 554 CCTAGG C^CTAGG
BalI 2710 TGGCCA TGG^CCA
BamHI 2382 GGATCC G^GATCC
BciVI 185 GTATCC GTATCC(6/5)
BclI 2872 TGATCA T^GATCA
BglI 1143 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 554 CCTAGG C^CTAGG
BsaAI 2344 CACGTA [CT]AC^GT[GA]
BsaHI 371 GGCGTC G[GA]^CG[CT]C
BsaI 124 GAGACC GGTCTC(1/5)
BsmI 3224 GAATGC GAATGC(1/-1)
BsrDI 2765 CATTGC GCAATG(2/0)
BssSI 2535 CTCGTG CACGAG(-5/-1)
BstUI 2106 CGCG CG^CG
DraII 3833 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 853 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2710 TGGCCA [CT]^GGCC[GA]
EcoO109I 3833 AGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 2872 TGATCA T^GATCA
HaeII 4601 GGCGCT [GA]GCGC^[CT]
Hin1I 371 GGCGTC G[GA]^CG[CT]C
HpaI 3717 GTTAAC GTT^AAC
MfeI 1042 CAATTG C^AATTG
MluNI 2710 TGGCCA TGG^CCA
MscI 2710 TGGCCA TGG^CCA
MspA1I 1341 CAGCTG C[AC]G^C[GT]G
MunI 1042 CAATTG C^AATTG
MvnI 2106 CGCG CG^CG
NdeI 1263 CATATG CA^TATG
PacI 837 TTAATTAA TTAAT^TAA
PaeR7I 1367 CTCGAG C^TCGAG
PflFI 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3833 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3058 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4073 ATTAAT AT^TAAT
PstI 43 CTGCAG CTGCA^G
PvuII 1341 CAGCTG CAG^CTG
SmlI 1367 CTCGAG C^T[CT][GA]AG
SphI 3228 GCATGC GCATG^C
SwaI 739 ATTTAAAT ATTT^AAAT
Tth111I 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1367 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1935 GTAGAC GT^[AC][GT]AC
2074 GTAGAC
AspHI 385 GAGCAC G[AT]GC[AT]^C
1258 GAGCAC
AvaI 490 CTCGGG C^[CT]CG[GA]G
1367 CTCGAG
BbsI 1122 GAAGAC GAAGAC(2/6)
2961 GTCTTC
BglII 1056 AGATCT A^GATCT
1400 AGATCT
BpmI 2450 CTCCAG CTGGAG(16/14)
1344 CTGGAG
BpuAI 1122 GAAGAC GAAGAC(2/6)
2961 GTCTTC
BseRI 2239 GAGGAG GAGGAG(10/8)
2296 GAGGAG
BsgI 1496 CTGCAC GTGCAG(16/14)
4021 CTGCAC
BsiHKAI 385 GAGCAC G[AT]GC[AT]^C
1258 GAGCAC
BsmBI 2176 GAGACG CGTCTC(1/5)
2242 GAGACG
BsoBI 490 CTCGGG C^[CT]CG[GA]G
1367 CTCGAG
BspLU11I 534 ACATGT A^CATGT
4655 ACATGT
EcoRV 70 GATATC GAT^ATC
582 GATATC
EcoT22I 3226 ATGCAT ATGCA^T
3593 ATGCAT
HgaI 2104 GACGC GACGC(5/10)
372 GCGTC
NsiI 3226 ATGCAT ATGCA^T
3593 ATGCAT
PleI 1289 GAGTC GAGTC(4/5)
3789 GAGTC
ScaI 2820 AGTACT AGT^ACT
3210 AGTACT
XcmI 1894 CCACTGTTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3022 CCAGTATTTTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I Asp718I
AvaII AviII BanI BbvI BfaI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BspHI BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI FspI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII XmnI