################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AspEI BanII BbeI BbrPI BbvCI BcgI BcnI BfrI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmFI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI HapII HpaII KasI KspI MamI MluI MroI MspI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 2106 CGCG CG^CG AcyI 371 GGCGTC G[GA]^CG[CT]C AlwNI 2161 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 4073 ATTAAT AT^TAAT AsnI 4073 ATTAAT AT^TAAT AspI 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 554 CCTAGG C^CTAGG BalI 2710 TGGCCA TGG^CCA BamHI 2382 GGATCC G^GATCC BciVI 185 GTATCC GTATCC(6/5) BclI 2872 TGATCA T^GATCA BglI 1143 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 554 CCTAGG C^CTAGG BsaAI 2344 CACGTA [CT]AC^GT[GA] BsaHI 371 GGCGTC G[GA]^CG[CT]C BsaI 124 GAGACC GGTCTC(1/5) BsmI 3224 GAATGC GAATGC(1/-1) BsrDI 2765 CATTGC GCAATG(2/0) BssSI 2535 CTCGTG CACGAG(-5/-1) BstUI 2106 CGCG CG^CG DraII 3833 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 853 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2710 TGGCCA [CT]^GGCC[GA] EcoO109I 3833 AGGACCT [GA]G^G[ACGT]CC[CT] FbaI 2872 TGATCA T^GATCA HaeII 4601 GGCGCT [GA]GCGC^[CT] Hin1I 371 GGCGTC G[GA]^CG[CT]C HpaI 3717 GTTAAC GTT^AAC MfeI 1042 CAATTG C^AATTG MluNI 2710 TGGCCA TGG^CCA MscI 2710 TGGCCA TGG^CCA MspA1I 1341 CAGCTG C[AC]G^C[GT]G MunI 1042 CAATTG C^AATTG MvnI 2106 CGCG CG^CG NdeI 1263 CATATG CA^TATG PacI 837 TTAATTAA TTAAT^TAA PaeR7I 1367 CTCGAG C^TCGAG PflFI 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 3833 AGGACCT [GA]G^G[AT]CC[CT] PshAI 3058 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4073 ATTAAT AT^TAAT PstI 43 CTGCAG CTGCA^G PvuII 1341 CAGCTG CAG^CTG SmlI 1367 CTCGAG C^T[CT][GA]AG SphI 3228 GCATGC GCATG^C SwaI 739 ATTTAAAT ATTT^AAAT Tth111I 2104 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1367 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1935 GTAGAC GT^[AC][GT]AC 2074 GTAGAC AspHI 385 GAGCAC G[AT]GC[AT]^C 1258 GAGCAC AvaI 490 CTCGGG C^[CT]CG[GA]G 1367 CTCGAG BbsI 1122 GAAGAC GAAGAC(2/6) 2961 GTCTTC BglII 1056 AGATCT A^GATCT 1400 AGATCT BpmI 2450 CTCCAG CTGGAG(16/14) 1344 CTGGAG BpuAI 1122 GAAGAC GAAGAC(2/6) 2961 GTCTTC BseRI 2239 GAGGAG GAGGAG(10/8) 2296 GAGGAG BsgI 1496 CTGCAC GTGCAG(16/14) 4021 CTGCAC BsiHKAI 385 GAGCAC G[AT]GC[AT]^C 1258 GAGCAC BsmBI 2176 GAGACG CGTCTC(1/5) 2242 GAGACG BsoBI 490 CTCGGG C^[CT]CG[GA]G 1367 CTCGAG BspLU11I 534 ACATGT A^CATGT 4655 ACATGT EcoRV 70 GATATC GAT^ATC 582 GATATC EcoT22I 3226 ATGCAT ATGCA^T 3593 ATGCAT HgaI 2104 GACGC GACGC(5/10) 372 GCGTC NsiI 3226 ATGCAT ATGCA^T 3593 ATGCAT PleI 1289 GAGTC GAGTC(4/5) 3789 GAGTC ScaI 2820 AGTACT AGT^ACT 3210 AGTACT XcmI 1894 CCACTGTTCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3022 CCAGTATTTTAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### Acc65I AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I Asp718I AvaII AviII BanI BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI FspI HaeIII HhaI HinP1I HincII HindII HindIII HinfI HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI