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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AlwNI Aor51HI ApaI AscI
AspI AvaI BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BglI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Cfr10I CpoI EaeI
EagI EclXI Eco47III Eco52I EcoO65I
FauI FseI HaeII HgaI Hin1I
KasI KspI MluI MluNI MroI
MscI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 2155 GGTACC G^GTACC
AccI 1965 GTAGAC GT^[AC][GT]AC
AciI 1556 CCGC CCGC(-3/-1)
AflII 2216 CTTAAG C^TTAAG
AflIII 2095 ACGTGT A^C[GA][CT]GT
AhdI 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 308 GTGCAC G^TGCAC
AocI 3548 CCTTAGG CC^T[ACGT]AGG
ApaLI 308 GTGCAC G^TGCAC
Asp718I 2155 GGTACC G^GTACC
AspEI 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1523 TGCGCA TGC^GCA
AvrII 2502 CCTAGG C^CTAGG
BclI 2942 TGATCA T^GATCA
BfrI 2216 CTTAAG C^TTAAG
BlnI 2502 CCTAGG C^CTAGG
BlpI 1558 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1558 GCTAAGC GC^T[ACGT]AGC
BsaAI 1730 TACGTG [CT]AC^GT[GA]
BsaI 1444 GAGACC GGTCTC(1/5)
BsgI 3024 GTGCAG GTGCAG(16/14)
BsmFI 691 GTCCC GGGAC(10/14)
BspDI 2671 ATCGAT AT^CGAT
BsrDI 2750 CATTGC GCAATG(2/0)
BssSI 2911 CACGAG CACGAG(-5/-1)
BstAPI 2522 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1990 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3548 CCTTAGG CC^T[ACGT]AGG
CelII 1558 GCTAAGC GC^T[ACGT]AGC
CfoI 1524 GCGC GCG^C
ClaI 2671 ATCGAT AT^CGAT
DraII 1121 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1404 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2812 CCATGG C^C[GA][CT]GG
Eam1105I 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3548 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1121 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 326 ATGCAT ATGCA^T
FbaI 2942 TGATCA T^GATCA
FspI 1523 TGCGCA TGC^GCA
HapII 437 CCGG C^CGG
HhaI 1524 GCGC GCG^C
HinP1I 1524 GCGC G^CGC
HindIII 3633 AAGCTT A^AGCTT
HpaI 4280 GTTAAC GTT^AAC
HpaII 437 CCGG C^CGG
KpnI 2155 GGTACC GGTAC^C
MfeI 2587 CAATTG C^AATTG
MspI 437 CCGG C^CGG
MunI 2587 CAATTG C^AATTG
NcoI 2812 CCATGG C^CATGG
NsiI 326 ATGCAT ATGCA^T
PacI 4521 TTAATTAA TTAAT^TAA
PflMI 3063 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1121 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1141 CTGCAG CTGCA^G
SacI 1397 GAGCTC GAGCT^C
ScaI 1588 AGTACT AGT^ACT
SphI 185 GCATGC GCATG^C
StuI 1281 AGGCCT AGG^CCT
SwaI 2412 ATTTAAAT ATTT^AAAT
Van91I 3063 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 227 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 3560 GATCC GGATC(4/5)
2129 GGATC
Asp700I 473 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
946 GAAATAATTC
BanI 1552 GGCACC G^G[CT][GA]CC
2155 GGTACC
BbsI 394 GAAGAC GAAGAC(2/6)
3084 GAAGAC
BpuAI 394 GAAGAC GAAGAC(2/6)
3084 GAAGAC
BsaBI 1091 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2124 GATATGGATC
BspHI 302 TCATGA T^CATGA
3640 TCATGA
DrdI 2090 GACAAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4016 GACAAGATTGTC
EcoNI 1137 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2502 CCTAGGGTAGG
EcoRI 1937 GAATTC G^AATTC
3491 GAATTC
EcoRV 99 GATATC GAT^ATC
1323 GATATC
HincII 4214 GTCAAC GT[CT]^[GA]AC
4280 GTTAAC
HindII 4214 GTCAAC GT[CT]^[GA]AC
4280 GTTAAC
MamI 1091 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2124 GATATGGATC
MspA1I 1019 CAGCTG C[AC]G^C[GT]G
3533 CAGCTG
NspI 185 GCATGC [GA]CATG^[CT]
3347 ACATGC
PleI 491 GAGTC GAGTC(4/5)
3661 GAGTC
PvuII 1019 CAGCTG CAG^CTG
3533 CAGCTG
RcaI 302 TCATGA T^CATGA
3640 TCATGA
SpeI 93 ACTAGT A^CTAGT
3524 ACTAGT
XmnI 473 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
946 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI AspHI AvaII BanII BbvI
BfaI BglII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII