################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccII AccIII AclI AcyI AgeI AlwNI Aor51HI ApaI AscI AspI AvaI BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I CpoI EaeI EagI EclXI Eco47III Eco52I EcoO65I FauI FseI HaeII HgaI Hin1I KasI KspI MluI MluNI MroI MscI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 2155 GGTACC G^GTACC AccI 1965 GTAGAC GT^[AC][GT]AC AciI 1556 CCGC CCGC(-3/-1) AflII 2216 CTTAAG C^TTAAG AflIII 2095 ACGTGT A^C[GA][CT]GT AhdI 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 308 GTGCAC G^TGCAC AocI 3548 CCTTAGG CC^T[ACGT]AGG ApaLI 308 GTGCAC G^TGCAC Asp718I 2155 GGTACC G^GTACC AspEI 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1523 TGCGCA TGC^GCA AvrII 2502 CCTAGG C^CTAGG BclI 2942 TGATCA T^GATCA BfrI 2216 CTTAAG C^TTAAG BlnI 2502 CCTAGG C^CTAGG BlpI 1558 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1558 GCTAAGC GC^T[ACGT]AGC BsaAI 1730 TACGTG [CT]AC^GT[GA] BsaI 1444 GAGACC GGTCTC(1/5) BsgI 3024 GTGCAG GTGCAG(16/14) BsmFI 691 GTCCC GGGAC(10/14) BspDI 2671 ATCGAT AT^CGAT BsrDI 2750 CATTGC GCAATG(2/0) BssSI 2911 CACGAG CACGAG(-5/-1) BstAPI 2522 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1990 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 3548 CCTTAGG CC^T[ACGT]AGG CelII 1558 GCTAAGC GC^T[ACGT]AGC CfoI 1524 GCGC GCG^C ClaI 2671 ATCGAT AT^CGAT DraII 1121 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 1404 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2812 CCATGG C^C[GA][CT]GG Eam1105I 3883 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3548 CCTTAGG CC^T[ACGT]AGG EcoO109I 1121 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT22I 326 ATGCAT ATGCA^T FbaI 2942 TGATCA T^GATCA FspI 1523 TGCGCA TGC^GCA HapII 437 CCGG C^CGG HhaI 1524 GCGC GCG^C HinP1I 1524 GCGC G^CGC HindIII 3633 AAGCTT A^AGCTT HpaI 4280 GTTAAC GTT^AAC HpaII 437 CCGG C^CGG KpnI 2155 GGTACC GGTAC^C MfeI 2587 CAATTG C^AATTG MspI 437 CCGG C^CGG MunI 2587 CAATTG C^AATTG NcoI 2812 CCATGG C^CATGG NsiI 326 ATGCAT ATGCA^T PacI 4521 TTAATTAA TTAAT^TAA PflMI 3063 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1121 AGGACCT [GA]G^G[AT]CC[CT] PstI 1141 CTGCAG CTGCA^G SacI 1397 GAGCTC GAGCT^C ScaI 1588 AGTACT AGT^ACT SphI 185 GCATGC GCATG^C StuI 1281 AGGCCT AGG^CCT SwaI 2412 ATTTAAAT ATTT^AAAT Van91I 3063 CCACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 227 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 3560 GATCC GGATC(4/5) 2129 GGATC Asp700I 473 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 946 GAAATAATTC BanI 1552 GGCACC G^G[CT][GA]CC 2155 GGTACC BbsI 394 GAAGAC GAAGAC(2/6) 3084 GAAGAC BpuAI 394 GAAGAC GAAGAC(2/6) 3084 GAAGAC BsaBI 1091 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2124 GATATGGATC BspHI 302 TCATGA T^CATGA 3640 TCATGA DrdI 2090 GACAAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4016 GACAAGATTGTC EcoNI 1137 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2502 CCTAGGGTAGG EcoRI 1937 GAATTC G^AATTC 3491 GAATTC EcoRV 99 GATATC GAT^ATC 1323 GATATC HincII 4214 GTCAAC GT[CT]^[GA]AC 4280 GTTAAC HindII 4214 GTCAAC GT[CT]^[GA]AC 4280 GTTAAC MamI 1091 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2124 GATATGGATC MspA1I 1019 CAGCTG C[AC]G^C[GT]G 3533 CAGCTG NspI 185 GCATGC [GA]CATG^[CT] 3347 ACATGC PleI 491 GAGTC GAGTC(4/5) 3661 GAGTC PvuII 1019 CAGCTG CAG^CTG 3533 CAGCTG RcaI 302 TCATGA T^CATGA 3640 TCATGA SpeI 93 ACTAGT A^CTAGT 3524 ACTAGT XmnI 473 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 946 GAAATAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI AspHI AvaII BanII BbvI BfaI BglII BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII