##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I AlwNI Aor51HI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BcgI BciVI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI CelII CfoI Cfr10I ClaI
CpoI DraIII EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 2148 GACGCC G[GA]^CG[CT]C
AhdI 100 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2069 CCTAAGG CC^T[ACGT]AGG
ApaI 2191 GGGCCC GGGCC^C
AseI 3705 ATTAAT AT^TAAT
AsnI 3705 ATTAAT AT^TAAT
Asp700I 411 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 100 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1726 GGACC G^G[AT]CC
BbrPI 3915 CACGTG CAC^GTG
BbvCI 1705 CCTCAGC CCTCAGC(-5/-2)
BpmI 1637 CTGGAG CTGGAG(16/14)
BsaAI 3915 CACGTG [CT]AC^GT[GA]
BsaHI 2148 GACGCC G[GA]^CG[CT]C
BsgI 1642 GTGCAG GTGCAG(16/14)
BspHI 837 TCATGA T^CATGA
BspLU11I 3101 ACATGT A^CATGT
BsrBI 34 CCGCTC CCGCTC(-3/-3)
BsrDI 2444 GCAATG GCAATG(2/0)
Bst1107I 751 GTATAC GTA^TAC
BstZ17I 751 GTATAC GTA^TAC
Bsu36I 2069 CCTAAGG CC^T[ACGT]AGG
DraII 2191 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 209 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2820 CCGTGG C^C[GA][CT]GG
Eam1105I 100 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2069 CCTAAGG CC^T[ACGT]AGG
EcoO109I 2191 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 773 GAATTC G^AATTC
HgaI 2148 GACGC GACGC(5/10)
Hin1I 2148 GACGCC G[GA]^CG[CT]C
MspA1I 263 CAGCTG C[AC]G^C[GT]G
NdeI 466 CATATG CA^TATG
PmaCI 3915 CACGTG CAC^GTG
PmlI 3915 CACGTG CAC^GTG
PshBI 3705 ATTAAT AT^TAAT
PvuII 263 CAGCTG CAG^CTG
RcaI 837 TCATGA T^CATGA
SphI 2825 GCATGC GCATG^C
StuI 1878 AGGCCT AGG^CCT
TaqI 1946 TCGA T^CGA
TthHB8I 1946 TCGA T^CGA
XbaI 798 TCTAGA T^CTAGA
XmnI 411 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 3101 ACATGT A^C[GA][CT]GT
3914 ACACGT
BbsI 2865 GAAGAC GAAGAC(2/6)
879 GTCTTC
BcnI 1751 CCGGG CC^[GC]GG
2219 CCCGG
BglII 1465 AGATCT A^GATCT
3009 AGATCT
BpuAI 2865 GAAGAC GAAGAC(2/6)
879 GTCTTC
BsaI 936 GGTCTC GGTCTC(1/5)
3412 GGTCTC
BstXI 1860 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2092 CCACAAACTTGG
FauI 33 CCCGC CCCGC(4/6)
1839 CCCGC
HapII 1751 CCGG C^CGG
2220 CCGG
HpaII 1751 CCGG C^CGG
2220 CCGG
MspI 1751 CCGG C^CGG
2220 CCGG
NciI 1751 CCGGG CC^[GC]GG
2219 CCCGG
PstI 6 CTGCAG CTGCA^G
291 CTGCAG
SacI 1822 GAGCTC GAGCT^C
3643 GAGCTC
SapI 3640 GAAGAGC GCTCTTC(1/4)
3645 GCTCTTC
SfaNI 1833 GATGC GCATC(5/9)
3023 GCATC
XcmI 153 CCACTAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2092 CCACAAACTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI BanII BbvI
BclI BfaI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII