##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BclI BcnI BglI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
Bsp1407I BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI Bsu36I CfoI
Cfr10I CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AclI 2132 AACGTT AA^CGTT
AflII 1140 CTTAAG C^TTAAG
AflIII 1697 ACATGT A^C[GA][CT]GT
Alw44I 655 GTGCAC G^TGCAC
ApaLI 655 GTGCAC G^TGCAC
AseI 3485 ATTAAT AT^TAAT
AsnI 3485 ATTAAT AT^TAAT
AvaI 21 CCCGAG C^[CT]CG[GA]G
BanI 701 GGTGCC G^G[CT][GA]CC
BfrI 1140 CTTAAG C^TTAAG
BglII 1390 AGATCT A^GATCT
BsaI 3937 GAGACC GGTCTC(1/5)
BsmAI 3937 GAGAC GTCTC(1/5)
BsoBI 21 CCCGAG C^[CT]CG[GA]G
BspDI 1989 ATCGAT AT^CGAT
BspHI 383 TCATGA T^CATGA
BspLU11I 1697 ACATGT A^CATGT
ClaI 1989 ATCGAT AT^CGAT
FauI 16 CCCGC CCCGC(4/6)
HgaI 305 GCGTC GACGC(5/10)
MslI 3840 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 3898 GCTAGC G^CTAGC
PpuMI 1135 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3485 ATTAAT AT^TAAT
Psp1406I 2132 AACGTT AA^CGTT
RcaI 383 TCATGA T^CATGA
SpeI 1374 ACTAGT A^CTAGT
XcmI 2443 CCATCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BbsI 2712 GAAGAC GAAGAC(2/6)
40 GTCTTC
BbvCI 1658 CCTCAGC CCTCAGC(-5/-2)
1332 GCTGAGG
BciVI 271 GTATCC GTATCC(6/5)
732 GTATCC
BlpI 426 GCTGAGC GC^T[ACGT]AGC
1433 GCTGAGC
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
1433 GCTGAGC
BpuAI 2712 GAAGAC GAAGAC(2/6)
40 GTCTTC
BsmI 607 GAATGC GAATGC(1/-1)
1624 GCATTC
BspMI 3940 ACCTGC ACCTGC(4/8)
1250 GCAGGT
Bst1107I 648 GTATAC GTA^TAC
3006 GTATAC
BstXI 637 CCATGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3839 CCATCTTTGTGG
BstZ17I 648 GTATAC GTA^TAC
3006 GTATAC
CelII 426 GCTGAGC GC^T[ACGT]AGC
1433 GCTGAGC
DraII 1135 AGGACCT [GA]G^G[ACGT]CC[CT]
2992 AGGCCCC
EcoNI 2010 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2496 CCTTTCCCAGG
EcoO109I 1135 AGGACCT [GA]G^G[ACGT]CC[CT]
2992 AGGCCCC
EcoRV 561 GATATC GAT^ATC
2750 GATATC
HindIII 900 AAGCTT A^AGCTT
3068 AAGCTT
NcoI 73 CCATGG C^CATGG
435 CCATGG
NspI 315 GCATGT [GA]CATG^[CT]
1697 ACATGT
PflMI 495 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1995 CCATTTGGTGG
PstI 421 CTGCAG CTGCA^G
739 CTGCAG
SacI 429 GAGCTC GAGCT^C
2306 GAGCTC
SapI 1428 GAAGAGC GCTCTTC(1/4)
2673 GAAGAGC
Van91I 495 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1995 CCATTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI