##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BclI BcnI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AciI 49 CCGC CCGC(-3/-1)
AflII 144 CTTAAG C^TTAAG
AflIII 1080 ACATGT A^C[GA][CT]GT
AlwNI 677 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1270 CCTCAGG CC^T[ACGT]AGG
AseI 1905 ATTAAT AT^TAAT
AsnI 1905 ATTAAT AT^TAAT
AspI 1702 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 584 CGATGAGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1125 GGATAC GTATCC(6/5)
BfrI 144 CTTAAG C^TTAAG
BpmI 1560 CTGGAG CTGGAG(16/14)
BsaBI 1968 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1032 GAGACC GGTCTC(1/5)
BseRI 52 CTCCTC GAGGAG(10/8)
BsgI 998 CTGCAC GTGCAG(16/14)
BspLU11I 1080 ACATGT A^CATGT
BsrBI 49 CCGCTC CCGCTC(-3/-3)
BssSI 1489 CACGAG CACGAG(-5/-1)
Bsu36I 1270 CCTCAGG CC^T[ACGT]AGG
EarI 1392 GAAGAG CTCTTC(1/4)
Eco81I 1270 CCTCAGG CC^T[ACGT]AGG
EcoRI 1476 GAATTC G^AATTC
FauI 48 CCCGC CCCGC(4/6)
HapII 82 CCGG C^CGG
HgaI 1228 GACGC GACGC(5/10)
HpaII 82 CCGG C^CGG
HphI 1308 GGTGA GGTGA(8/7)
Ksp632I 1392 GAAGAG CTCTTC(1/4)
MaeII 1583 ACGT A^CGT
MamI 1968 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1928 CAATTG C^AATTG
MspI 82 CCGG C^CGG
MunI 1928 CAATTG C^AATTG
NheI 1912 GCTAGC G^CTAGC
PflFI 1702 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2333 GACTC GAGTC(4/5)
PshBI 1905 ATTAAT AT^TAAT
SapI 1392 GAAGAGC GCTCTTC(1/4)
SexAI 231 ACCAGGT A^CC[AT]GGT
SfcI 1734 CTGTAG C^T[GA][CT]AG
SphI 2283 GCATGC GCATG^C
SwaI 1762 ATTTAAAT ATTT^AAAT
Tth111I 1702 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1964 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 1869 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2267 GAACAACTTC
BglII 1967 AGATCT A^GATCT
2414 AGATCT
BmyI 886 GTGCCC G[AGT]GC[ACT]^C
1135 GTGCCC
BsmI 1161 GAATGC GAATGC(1/-1)
1891 GCATTC
Bsp1286I 886 GTGCCC G[AGT]GC[ACT]^C
1135 GTGCCC
BstAPI 676 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2146 GCAGTGTTTGC
DraIII 230 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1024 CACACTGTG
EcoRV 667 GATATC GAT^ATC
1938 GATATC
HincII 940 GTCAAC GT[CT]^[GA]AC
1095 GTTGAC
HindII 940 GTCAAC GT[CT]^[GA]AC
1095 GTTGAC
MslI 386 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1068 CACAATAATG
PpuMI 517 AGGACCC [GA]G^G[AT]CC[CT]
1432 GGGTCCC
SspI 1498 AATATT AAT^ATT
2375 AATATT
TaqI 1150 TCGA T^CGA
2164 TCGA
TthHB8I 1150 TCGA T^CGA
2164 TCGA
XcmI 776 CCACAATTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1898 CCAATTCATTAATGG
XmnI 1869 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2267 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanI BbvI BfaI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EcoO109I EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII