##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI AlwNI Aor51HI AscI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BcgI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI BstAPI BstEII BstPI
BstUI BstXI CelII CfoI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KspI MluI MroI MvnI
NaeI NarI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 3279 CTTAAG C^TTAAG
Alw44I 342 GTGCAC G^TGCAC
ApaI 1251 GGGCCC GGGCC^C
ApaLI 342 GTGCAC G^TGCAC
AseI 2526 ATTAAT AT^TAAT
AsnI 2526 ATTAAT AT^TAAT
BalI 3809 TGGCCA TGG^CCA
BamHI 4034 GGATCC G^GATCC
BfrI 3279 CTTAAG C^TTAAG
BsaAI 3226 CACGTA [CT]AC^GT[GA]
BsaI 835 GAGACC GGTCTC(1/5)
BsgI 1750 GTGCAG GTGCAG(16/14)
BsmI 1802 GAATGC GAATGC(1/-1)
BspDI 908 ATCGAT AT^CGAT
BspHI 776 TCATGA T^CATGA
BspLU11I 2355 ACATGT A^CATGT
BspMI 2228 GCAGGT ACCTGC(4/8)
BsrDI 3572 GCAATG GCAATG(2/0)
BsrFI 323 ACCGGC [GA]^CCGG[CT]
BstBI 3485 TTCGAA TT^CGAA
Cfr10I 323 ACCGGC [GA]^CCGG[CT]
ClaI 908 ATCGAT AT^CGAT
DsaI 32 CCATGG C^C[GA][CT]GG
EaeI 3809 TGGCCA [CT]^GGCC[GA]
EcoNI 1075 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3110 GAATTC G^AATTC
EcoRV 905 GATATC GAT^ATC
EcoT22I 2623 ATGCAT ATGCA^T
HindIII 1997 AAGCTT A^AGCTT
HpaI 3006 GTTAAC GTT^AAC
MluNI 3809 TGGCCA TGG^CCA
MscI 3809 TGGCCA TGG^CCA
MspA1I 487 CAGCTG C[AC]G^C[GT]G
NcoI 32 CCATGG C^CATGG
NsiI 2623 ATGCAT ATGCA^T
NspV 3485 TTCGAA TT^CGAA
PaeR7I 222 CTCGAG C^TCGAG
PshBI 2526 ATTAAT AT^TAAT
PstI 3596 CTGCAG CTGCA^G
PvuII 487 CAGCTG CAG^CTG
RcaI 776 TCATGA T^CATGA
SfuI 3485 TTCGAA TT^CGAA
SmaI 599 CCCGGG CCC^GGG
SphI 2621 GCATGC GCATG^C
SspI 4009 AATATT AAT^ATT
XhoI 222 CTCGAG C^TCGAG
XmaCI 599 CCCGGG C^CCGGG
XmaI 599 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 2291 GGTACC G^GTACC
2953 GGTACC
AccI 3066 GTATAC GT^[AC][GT]AC
3679 GTATAC
AflIII 2355 ACATGT A^C[GA][CT]GT
3225 ACACGT
AocI 1075 CCTGAGG CC^T[ACGT]AGG
1663 CCTGAGG
Asp718I 2291 GGTACC G^GTACC
2953 GGTACC
AvaI 222 CTCGAG C^[CT]CG[GA]G
599 CCCGGG
BcnI 599 CCCGG CC^[GC]GG
600 CCGGG
BglI 1204 GCCCACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1898 GCCTGCCTGGC
BsaBI 1832 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3094 GATTCAGATC
BsoBI 222 CTCGAG C^[CT]CG[GA]G
599 CCCGGG
Bsp1407I 2585 TGTACA T^GTACA
3960 TGTACA
BsrGI 2585 TGTACA T^GTACA
3960 TGTACA
Bst1107I 3066 GTATAC GTA^TAC
3679 GTATAC
BstZ17I 3066 GTATAC GTA^TAC
3679 GTATAC
Bsu36I 1075 CCTGAGG CC^T[ACGT]AGG
1663 CCTGAGG
Eco81I 1075 CCTGAGG CC^T[ACGT]AGG
1663 CCTGAGG
FauI 923 GCGGG CCCGC(4/6)
1362 GCGGG
HapII 324 CCGG C^CGG
600 CCGG
HpaII 324 CCGG C^CGG
600 CCGG
KpnI 2291 GGTACC GGTAC^C
2953 GGTACC
MamI 1832 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3094 GATTCAGATC
MfeI 2223 CAATTG C^AATTG
2990 CAATTG
MspI 324 CCGG C^CGG
600 CCGG
MunI 2223 CAATTG C^AATTG
2990 CAATTG
NciI 599 CCCGG CC^[GC]GG
600 CCGGG
NdeI 2589 CATATG CA^TATG
2900 CATATG
PflMI 1443 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3286 CCACTACCTGG
SacI 620 GAGCTC GAGCT^C
1414 GAGCTC
ScaI 1696 AGTACT AGT^ACT
3592 AGTACT
SspBI 2585 TGTACA T^GTACA
3960 TGTACA
Van91I 1443 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3286 CCACTACCTGG
XcmI 800 CCAAGCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1145 CCATCATGCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BciVI BclI BfaI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII