##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI BbrPI BclI
BfrI BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 381 GTCTAC GT^[AC][GT]AC
AccIII 883 TCCGGA T^CCGGA
AgeI 1303 ACCGGT A^CCGGT
Aor51HI 1193 AGCGCT AGC^GCT
AvrII 2168 CCTAGG C^CTAGG
BalI 1397 TGGCCA TGG^CCA
BciVI 1840 GTATCC GTATCC(6/5)
BlnI 2168 CCTAGG C^CTAGG
BseAI 883 TCCGGA T^CCGGA
BsiEI 176 CGGCCG CG[GA][CT]^CG
BsmBI 3196 CGTCTC CGTCTC(1/5)
BspEI 883 TCCGGA T^CCGGA
BsrDI 128 CATTGC GCAATG(2/0)
BstXI 3455 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1731 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 176 CGGCCG C^GGCCG
EclXI 176 CGGCCG C^GGCCG
Eco47III 1193 AGCGCT AGC^GCT
Eco52I 176 CGGCCG C^GGCCG
HincII 1227 GTCAAC GT[CT]^[GA]AC
HindII 1227 GTCAAC GT[CT]^[GA]AC
HindIII 2892 AAGCTT A^AGCTT
MluNI 1397 TGGCCA TGG^CCA
MroI 883 TCCGGA T^CCGGA
MscI 1397 TGGCCA TGG^CCA
MseI 3828 TTAA T^TAA
PaeR7I 3217 CTCGAG C^TCGAG
PinAI 1303 ACCGGT A^CCGGT
PshAI 2947 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2099 CCTGCAGG CCTGCA^GG
ScaI 1873 AGTACT AGT^ACT
SfiI 2784 GGCCAGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1864 GCATGC GCATG^C
Sse8387I 2099 CCTGCAGG CCTGCA^GG
TfiI 1422 GAATC G^A[AT]TC
Tru9I 3828 TTAA T^TAA
XcmI 955 CCACACTCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3217 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1088 GAATTT [GA]^AATT[CT]
3817 AAATTT
AflIII 1231 ACGTGT A^C[GA][CT]GT
1663 ACGTGT
AocI 3265 CCTCAGG CC^T[ACGT]AGG
3757 CCTGAGG
ApoI 1088 GAATTT [GA]^AATT[CT]
3817 AAATTT
Asp700I 287 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1688 GAAGATCTTC
AviII 124 TGCGCA TGC^GCA
1597 TGCGCA
BamHI 113 GGATCC G^GATCC
3682 GGATCC
BbsI 1256 GAAGAC GAAGAC(2/6)
2739 GAAGAC
BglII 1690 AGATCT A^GATCT
2708 AGATCT
BlpI 537 GCTGAGC GC^T[ACGT]AGC
2351 GCTCAGC
Bpu1102I 537 GCTGAGC GC^T[ACGT]AGC
2351 GCTCAGC
BpuAI 1256 GAAGAC GAAGAC(2/6)
2739 GAAGAC
Bsu36I 3265 CCTCAGG CC^T[ACGT]AGG
3757 CCTGAGG
CelII 537 GCTGAGC GC^T[ACGT]AGC
2351 GCTCAGC
DrdI 2909 GACAGAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3720 GACCTCCCGGTC
Eco81I 3265 CCTCAGG CC^T[ACGT]AGG
3757 CCTGAGG
FspI 124 TGCGCA TGC^GCA
1597 TGCGCA
NaeI 257 GCCGGC GCC^GGC
706 GCCGGC
NgoMIV 257 GCCGGC G^CCGGC
706 GCCGGC
PflMI 2686 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3425 CCACAGCCTGG
SexAI 1167 ACCTGGT A^CC[AT]GGT
1486 ACCTGGT
SmaI 12 CCCGGG CCC^GGG
421 CCCGGG
StuI 3695 AGGCCT AGG^CCT
3754 AGGCCT
Tsp509I 1089 AATT ^AATT
3818 AATT
Van91I 2686 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3425 CCACAGCCTGG
XmaCI 12 CCCGGG C^CCGGG
421 CCCGGG
XmaI 12 CCCGGG C^CCGGG
421 CCCGGG
XmnI 287 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1688 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII