##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII BalI
BamHI BanI BbeI BbrPI BcgI
BglI BlpI Bpu1102I BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BspMI
BsrBI BsrGI BssSI BstAPI BstBI
BstEII BstPI Bsu36I CelII CpoI
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI FauI FseI FspI
HaeII Hin1I HindIII KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PleI
PmaCI PmlI PshAI PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 834 CGCG CG^CG
AciI 988 CCGC CCGC(-3/-1)
AclI 2471 AACGTT AA^CGTT
Alw44I 2162 GTGCAC G^TGCAC
AlwNI 270 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2162 GTGCAC G^TGCAC
AspI 268 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3441 CCTAGG C^CTAGG
BanII 1030 GAGCTC G[GA]GC[CT]^C
BbvCI 1354 CCTCAGC CCTCAGC(-5/-2)
BciVI 4066 GTATCC GTATCC(6/5)
BglII 3354 AGATCT A^GATCT
BlnI 3441 CCTAGG C^CTAGG
BpmI 3808 CTGGAG CTGGAG(16/14)
BsaAI 2144 TACGTG [CT]AC^GT[GA]
BsaBI 298 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 323 ACCGGA [AT]^CCGG[AT]
BseRI 488 GAGGAG GAGGAG(10/8)
BsiYI 294 CCACGATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 294 CCACGATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1084 GAATGC GAATGC(1/-1)
BspDI 669 ATCGAT AT^CGAT
BsrFI 3937 GCCGGT [GA]^CCGG[CT]
BssHII 833 GCGCGC G^CGCGC
Bst1107I 722 GTATAC GTA^TAC
BstNI 33 CCAGG CC^[AT]GG
BstUI 834 CGCG CG^CG
BstXI 807 CCACAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 722 GTATAC GTA^TAC
Cfr10I 3937 GCCGGT [GA]^CCGG[CT]
ClaI 669 ATCGAT AT^CGAT
DraIII 295 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRII 33 CCAGG ^CC[AT]GG
EcoRV 2277 GATATC GAT^ATC
EcoT22I 1509 ATGCAT ATGCA^T
HgaI 659 GCGTC GACGC(5/10)
HinfI 2618 GAATC G^A[ACGT]TC
MamI 298 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 33 CCAGG CC^[AT]GG
MvnI 834 CGCG CG^CG
NdeI 2241 CATATG CA^TATG
NsiI 1509 ATGCAT ATGCA^T
PflFI 268 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 294 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1100 GTTTAAAC GTTT^AAAC
Psp1406I 2471 AACGTT AA^CGTT
SacI 1030 GAGCTC GAGCT^C
StuI 49 AGGCCT AGG^CCT
TfiI 2618 GAATC G^A[AT]TC
Tth111I 268 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 294 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 383 ACATGT A^C[GA][CT]GT
2201 ACATGT
Asp700I 2694 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4019 GAAAGGCTTC
BbvI 191 GCTGC GCAGC(8/12)
364 GCTGC
BclI 2062 TGATCA T^GATCA
2859 TGATCA
BcnI 146 CCCGG CC^[GC]GG
1438 CCGGG
BspLU11I 383 ACATGT A^CATGT
2201 ACATGT
BsrDI 3208 CATTGC GCAATG(2/0)
3284 GCAATG
BstYI 1571 GGATCT [GA]^GATC[CT]
3354 AGATCT
DraII 1931 GGGACCC [GA]G^G[ACGT]CC[CT]
3456 AGGTCCC
EcoO109I 1931 GGGACCC [GA]G^G[ACGT]CC[CT]
3456 AGGTCCC
FbaI 2062 TGATCA T^GATCA
2859 TGATCA
Fnu4HI 191 GCTGC GC^[ACGT]GC
364 GCTGC
HincII 1268 GTTAAC GT[CT]^[GA]AC
2847 GTTAAC
HindII 1268 GTTAAC GT[CT]^[GA]AC
2847 GTTAAC
HpaI 1268 GTTAAC GTT^AAC
2847 GTTAAC
ItaI 191 GCTGC GC^[ACGT]GC
364 GCTGC
MflI 1571 GGATCT [GA]^GATC[CT]
3354 AGATCT
NciI 146 CCCGG CC^[GC]GG
1438 CCGGG
PpuMI 1931 GGGACCC [GA]G^G[AT]CC[CT]
3456 AGGTCCC
SapI 888 GAAGAGC GCTCTTC(1/4)
1716 GAAGAGC
ScaI 1799 AGTACT AGT^ACT
2931 AGTACT
SfaNI 231 GATGC GCATC(5/9)
2169 GATGC
SfcI 3501 CTACAG C^T[GA][CT]AG
3533 CTGTAG
SpeI 1335 ACTAGT A^CTAGT
2937 ACTAGT
TseI 191 GCTGC G^C[AT]GC
364 GCTGC
XhoII 1571 GGATCT [GA]^GATC[CT]
3354 AGATCT
XmnI 2694 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4019 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BbsI BfaI BfrI BmyI
BpuAI BsaJI BsiHKAI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoT14I FokI HaeIII HapII
HhaI HinP1I HpaII HphI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MwoI
NdeII NlaIII NlaIV NspI PshBI
RsaI Sau3AI Sau96I ScrFI SmlI
SspI StyI TaqI Tru9I Tsp45I
Tsp509I TspRI TthHB8I