##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AspI BbeI BbrPI
BciVI BclI BglII BsaAI BsaBI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoT22I FbaI FseI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 699 CGCG CG^CG
AcsI 280 GAATTT [GA]^AATT[CT]
AflII 558 CTTAAG C^TTAAG
Alw44I 2126 GTGCAC G^TGCAC
ApaLI 2126 GTGCAC G^TGCAC
ApoI 280 GAATTT [GA]^AATT[CT]
AseI 794 ATTAAT AT^TAAT
AsnI 794 ATTAAT AT^TAAT
AviII 1268 TGCGCA TGC^GCA
AvrII 3199 CCTAGG C^CTAGG
BamHI 724 GGATCC G^GATCC
BanI 2621 GGTGCC G^G[CT][GA]CC
BbsI 2063 GAAGAC GAAGAC(2/6)
BcgI 2883 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 558 CTTAAG C^TTAAG
BlnI 3199 CCTAGG C^CTAGG
BpuAI 2063 GAAGAC GAAGAC(2/6)
BsmBI 136 CGTCTC CGTCTC(1/5)
BsmI 1983 GAATGC GAATGC(1/-1)
BspHI 473 TCATGA T^CATGA
BsrBI 948 GAGCGG CCGCTC(-3/-3)
BsrDI 1300 CATTGC GCAATG(2/0)
BstUI 699 CGCG CG^CG
DraI 619 TTTAAA TTT^AAA
DraIII 2092 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 698 CCGCGG C^C[GA][CT]GG
EcoRV 813 GATATC GAT^ATC
FspI 1268 TGCGCA TGC^GCA
KspI 698 CCGCGG CCGC^GG
MaeII 1882 ACGT A^CGT
MslI 2131 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 699 CGCG CG^CG
PaeR7I 3146 CTCGAG C^TCGAG
PshBI 794 ATTAAT AT^TAAT
RcaI 473 TCATGA T^CATGA
SacII 698 CCGCGG CCGC^GG
SapI 3111 GCTCTTC GCTCTTC(1/4)
SbfI 1422 CCTGCAGG CCTGCA^GG
SfiI 2392 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1422 CCTGCAGG CCTGCA^GG
XbaI 419 TCTAGA T^CTAGA
XcmI 1076 CCACCATGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3146 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 2279 GGCGTC G[GA]^CG[CT]C
2957 GACGCC
AfaI 629 GTAC GT^AC
3025 GTAC
AflIII 735 ACATGT A^C[GA][CT]GT
770 ACATGT
BalI 115 TGGCCA TGG^CCA
2907 TGGCCA
BsaHI 2279 GGCGTC G[GA]^CG[CT]C
2957 GACGCC
BsaI 128 GAGACC GGTCTC(1/5)
2639 GAGACC
BspLU11I 735 ACATGT A^CATGT
770 ACATGT
BspMI 1265 ACCTGC ACCTGC(4/8)
1886 GCAGGT
HaeII 952 GGCGCT [GA]GCGC^[CT]
2329 GGCGCT
Hin1I 2279 GGCGTC G[GA]^CG[CT]C
2957 GACGCC
MluNI 115 TGGCCA TGG^CCA
2907 TGGCCA
MscI 115 TGGCCA TGG^CCA
2907 TGGCCA
PshAI 130 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1622 GACTCAGGTC
RsaI 629 GTAC GT^AC
3025 GTAC
SmaI 1923 CCCGGG CCC^GGG
2004 CCCGGG
StuI 303 AGGCCT AGG^CCT
2400 AGGCCT
TfiI 392 GAATC G^A[AT]TC
681 GAATC
Tsp509I 158 AATT ^AATT
281 AATT
XmaCI 1923 CCCGGG C^CCGGG
2004 CCCGGG
XmaI 1923 CCCGGG C^CCGGG
2004 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII