##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AocI Aor51HI ApaI AscI
AvaI AvaII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BcnI
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI Bsu36I Cac8I Cfr10I
Cfr13I ClaI CpoI DraII DrdI
DsaI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT14I EcoT22I FseI HapII
HgaI Hin1I HpaI HpaII KasI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
Sau96I SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1276 GGTACC G^GTACC
AciI 866 GCGG CCGC(-3/-1)
AflII 2228 CTTAAG C^TTAAG
AflIII 1236 ACATGT A^C[GA][CT]GT
AhdI 537 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1536 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1435 ATTAAT AT^TAAT
AsnI 1435 ATTAAT AT^TAAT
Asp718I 1276 GGTACC G^GTACC
AspEI 537 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 714 TGCGCA TGC^GCA
BfrI 2228 CTTAAG C^TTAAG
BglI 1544 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1862 AGATCT A^GATCT
BlpI 1796 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1796 GCTGAGC GC^T[ACGT]AGC
BseRI 1894 GAGGAG GAGGAG(10/8)
BspHI 2021 TCATGA T^CATGA
BspLU11I 1236 ACATGT A^CATGT
BspMI 1257 ACCTGC ACCTGC(4/8)
BstF5I 1429 CATCC GGATG(2/0)
BstYI 1862 AGATCT [GA]^GATC[CT]
CelII 1796 GCTGAGC GC^T[ACGT]AGC
Eam1105I 537 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 866 GCGGG CCCGC(4/6)
FokI 1429 CATCC GGATG(9/13)
FspI 714 TGCGCA TGC^GCA
HaeII 628 AGCGCC [GA]GCGC^[CT]
HindIII 1411 AAGCTT A^AGCTT
KpnI 1276 GGTACC GGTAC^C
MflI 1862 AGATCT [GA]^GATC[CT]
NdeI 1245 CATATG CA^TATG
PflMI 1350 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1435 ATTAAT AT^TAAT
RcaI 2021 TCATGA T^CATGA
SacI 1682 GAGCTC GAGCT^C
SapI 365 GAAGAGC GCTCTTC(1/4)
ScaI 983 AGTACT AGT^ACT
SspI 1362 AATATT AAT^ATT
TaqI 900 TCGA T^CGA
TfiI 71 GAATC G^A[AT]TC
TthHB8I 900 TCGA T^CGA
Van91I 1350 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1862 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1240 GTATAC GT^[AC][GT]AC
2027 GTATAC
Alw44I 460 GTGCAC G^TGCAC
1268 GTGCAC
AlwI 1561 GATCC GGATC(4/5)
2222 GGATC
ApaLI 460 GTGCAC G^TGCAC
1268 GTGCAC
AspI 1478 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2437 GACCCTGTC
BanII 1682 GAGCTC G[GA]GC[CT]^C
2207 GAGCCC
BclI 1423 TGATCA T^GATCA
2398 TGATCA
BsaI 87 GAGACC GGTCTC(1/5)
2435 GAGACC
BsmFI 1591 GGGAC GGGAC(10/14)
1722 GGGAC
BsrDI 1043 CATTGC GCAATG(2/0)
2407 CATTGC
Bst1107I 1240 GTATAC GTA^TAC
2027 GTATAC
BstXI 774 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1712 CCAAAGTGCTGG
BstZ17I 1240 GTATAC GTA^TAC
2027 GTATAC
DraI 1005 TTTAAA TTT^AAA
1135 TTTAAA
EarI 720 CTCTTC CTCTTC(1/4)
365 GAAGAG
FbaI 1423 TGATCA T^GATCA
2398 TGATCA
HincII 815 GTCAAC GT[CT]^[GA]AC
2424 GTTGAC
HindII 815 GTCAAC GT[CT]^[GA]AC
2424 GTTGAC
Ksp632I 720 CTCTTC CTCTTC(1/4)
365 GAAGAG
MaeII 1263 ACGT A^CGT
1982 ACGT
PflFI 1478 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2437 GACCCTGTC
StuI 284 AGGCCT AGG^CCT
788 AGGCCT
Tsp45I 1494 GTCAC ^GT[GC]AC
1942 GTGAC
Tth111I 1478 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2437 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI BanI BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstAPI
BstNI CfoI DdeI DpnII DraIII
EcoRI EcoRII Fnu4HI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI ScrFI SfaNI SfcI SmlI
Tru9I TseI Tsp509I TspRI XmnI