##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AvrII BamHI BbsI BcgI
BclI BlnI BpuAI BsmI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MfeI MluI MunI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 191 GGTACC G^GTACC
ApaI 3251 GGGCCC GGGCC^C
Asp718I 191 GGTACC G^GTACC
AspI 4247 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3367 GGCGCC GGCGC^C
BglI 53 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1082 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1082 GCTGAGC GC^T[ACGT]AGC
BsaBI 2312 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1294 CGTACG C^GTACG
BsrDI 1144 GCAATG GCAATG(2/0)
CelII 1082 GCTGAGC GC^T[ACGT]AGC
DraI 3694 TTTAAA TTT^AAA
KasI 3367 GGCGCC G^GCGCC
KpnI 191 GGTACC GGTAC^C
KspI 134 CCGCGG CCGC^GG
MamI 2312 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3367 GGCGCC GG^CGCC
NcoI 274 CCATGG C^CATGG
NdeI 468 CATATG CA^TATG
PflFI 4247 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 893 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 134 CCGCGG CCGC^GG
SfoI 3367 GGCGCC GGC^GCC
SspI 3622 AATATT AAT^ATT
Tth111I 4247 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 893 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 739 TCCGGA T^CCGGA
1870 TCCGGA
AflII 1878 CTTAAG C^TTAAG
3959 CTTAAG
Alw44I 852 GTGCAC G^TGCAC
4125 GTGCAC
AocI 2627 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
ApaLI 852 GTGCAC G^TGCAC
4125 GTGCAC
Asp700I 1446 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2582 GAACGCCTTC
BbrPI 2222 CACGTG CAC^GTG
3162 CACGTG
BbvCI 1193 CCTCAGC CCTCAGC(-5/-2)
3646 GCTGAGG
BciVI 1712 GGATAC GTATCC(6/5)
4099 GGATAC
BfaI 2800 CTAG C^TAG
4092 CTAG
BfrI 1878 CTTAAG C^TTAAG
3959 CTTAAG
BsaAI 2222 CACGTG [CT]AC^GT[GA]
3162 CACGTG
BseAI 739 TCCGGA T^CCGGA
1870 TCCGGA
Bsp1407I 1324 TGTACA T^GTACA
3110 TGTACA
BspEI 739 TCCGGA T^CCGGA
1870 TCCGGA
BsrBI 593 GAGCGG CCGCTC(-3/-3)
870 GAGCGG
BsrGI 1324 TGTACA T^GTACA
3110 TGTACA
Bsu36I 2627 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
DraIII 2100 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2660 CACCTCGTG
DrdI 1017 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3024 GACGCCAAAGTC
EagI 2072 CGGCCG C^GGCCG
2235 CGGCCG
EclXI 2072 CGGCCG C^GGCCG
2235 CGGCCG
Eco52I 2072 CGGCCG C^GGCCG
2235 CGGCCG
Eco81I 2627 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
EcoRI 1791 GAATTC G^AATTC
3350 GAATTC
HaeII 173 GGCGCT [GA]GCGC^[CT]
3367 GGCGCC
HindIII 240 AAGCTT A^AGCTT
2176 AAGCTT
MaeI 2800 CTAG C^TAG
4092 CTAG
MroI 739 TCCGGA T^CCGGA
1870 TCCGGA
NaeI 460 GCCGGC GCC^GGC
2148 GCCGGC
NgoMIV 460 GCCGGC G^CCGGC
2148 GCCGGC
PmaCI 2222 CACGTG CAC^GTG
3162 CACGTG
PmlI 2222 CACGTG CAC^GTG
3162 CACGTG
SapI 1511 GCTCTTC GCTCTTC(1/4)
2852 GCTCTTC
SexAI 967 ACCTGGT A^CC[AT]GGT
2101 ACCAGGT
SmaI 1671 CCCGGG CCC^GGG
4180 CCCGGG
SspBI 1324 TGTACA T^GTACA
3110 TGTACA
XcmI 1768 CCAGGAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3716 CCAAGGACACAGTGG
XmaCI 1671 CCCGGG C^CCGGG
4180 CCCGGG
XmaI 1671 CCCGGG C^CCGGG
4180 CCCGGG
XmnI 1446 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2582 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvI BcnI BglII BmyI
BpmI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII