##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp718I AviII BbeI BciVI BfrI
BglII BsaBI BsiWI BspDI BspLU11I
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DrdI Eco47III EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MamI MfeI MluI MunI NarI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SalI ScaI SfiI
SfoI SfuI SnaBI SpeI SphI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 1618 TCCGGA T^CCGGA
AcyI 203 GACGCC G[GA]^CG[CT]C
AhdI 998 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2763 CCTGAGG CC^T[ACGT]AGG
Asp700I 1321 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 998 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1208 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2750 CCTAGG C^CTAGG
BbvCI 4172 GCTGAGG CCTCAGC(-5/-2)
BcgI 3178 GCATCTCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2199 TGATCA T^GATCA
BlnI 2750 CCTAGG C^CTAGG
BlpI 3022 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3022 GCTCAGC GC^T[ACGT]AGC
BsaHI 203 GACGCC G[GA]^CG[CT]C
BseAI 1618 TCCGGA T^CCGGA
BsmBI 4061 CGTCTC CGTCTC(1/5)
BsmI 2327 GCATTC GAATGC(1/-1)
Bsp1407I 987 TGTACA T^GTACA
BspEI 1618 TCCGGA T^CCGGA
BspHI 3273 TCATGA T^CATGA
BsrGI 987 TGTACA T^GTACA
BssSI 269 CTCGTG CACGAG(-5/-1)
BstAPI 3159 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2763 CCTGAGG CC^T[ACGT]AGG
CelII 3022 GCTCAGC GC^T[ACGT]AGC
Eam1105I 998 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2763 CCTGAGG CC^T[ACGT]AGG
EcoRI 2834 GAATTC G^AATTC
FbaI 2199 TGATCA T^GATCA
Hin1I 203 GACGCC G[GA]^CG[CT]C
MroI 1618 TCCGGA T^CCGGA
NdeI 4239 CATATG CA^TATG
NheI 139 GCTAGC G^CTAGC
NspI 2217 ACATGC [GA]CATG^[CT]
PaeR7I 3794 CTCGAG C^TCGAG
PflFI 1208 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2000 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3273 TCATGA T^CATGA
SbfI 2488 CCTGCAGG CCTGCA^GG
SgrAI 4 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 2488 CCTGCAGG CCTGCA^GG
SspBI 987 TGTACA T^GTACA
SwaI 4376 ATTTAAAT ATTT^AAAT
Tth111I 1208 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2404 TCTAGA T^CTAGA
XhoI 3794 CTCGAG C^TCGAG
XmnI 1321 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1728 ACACGT A^C[GA][CT]GT
3940 ACACGT
Alw44I 1860 GTGCAC G^TGCAC
4502 GTGCAC
AlwNI 1922 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3248 CAGGCACTG
ApaLI 1860 GTGCAC G^TGCAC
4502 GTGCAC
BamHI 1185 GGATCC G^GATCC
1585 GGATCC
BbrPI 1679 CACGTG CAC^GTG
3941 CACGTG
BsaI 1827 GAGACC GGTCTC(1/5)
3238 GGTCTC
BsaWI 447 TCCGGT [AT]^CCGG[AT]
1618 TCCGGA
BsrFI 5 GCCGGC [GA]^CCGG[CT]
570 GCCGGC
BssHII 1 GCGCGC G^CGCGC
47 GCGCGC
BstYI 1185 GGATCC [GA]^GATC[CT]
1585 GGATCC
Cfr10I 5 GCCGGC [GA]^CCGG[CT]
570 GCCGGC
DraIII 1854 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4259 CACCTTGTG
HaeII 90 GGCGCT [GA]GCGC^[CT]
2935 AGCGCC
HincII 1214 GTCAAC GT[CT]^[GA]AC
1982 GTTGAC
HindII 1214 GTCAAC GT[CT]^[GA]AC
1982 GTTGAC
MflI 1185 GGATCC [GA]^GATC[CT]
1585 GGATCC
NaeI 5 GCCGGC GCC^GGC
570 GCCGGC
NcoI 1491 CCATGG C^CATGG
1822 CCATGG
NgoMIV 5 GCCGGC G^CCGGC
570 GCCGGC
PmaCI 1679 CACGTG CAC^GTG
3941 CACGTG
PmlI 1679 CACGTG CAC^GTG
3941 CACGTG
PpuMI 70 GGGACCT [GA]G^G[AT]CC[CT]
499 AGGACCT
SapI 2280 GCTCTTC GCTCTTC(1/4)
3136 GCTCTTC
SexAI 1209 ACCTGGT A^CC[AT]GGT
1855 ACCTGGT
XhoII 1185 GGATCC [GA]^GATC[CT]
1585 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XmaCI XmaI