##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AclI AgeI ApaI AscI Asp700I
AspI AviII AvrII BbrPI BcgI
BclI BlnI BsiWI BsmBI BsmI
BspHI BspLU11I BsrDI Bst1107I BstEII
BstPI BstZ17I CpoI DraIII EcoO65I
EcoRV EcoT22I FbaI FseI FspI
MluI NcoI NheI NotI NruI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII ScaI SfiI SgrAI
SnaBI SpeI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2727 GGTACC G^GTACC
AflII 2314 CTTAAG C^TTAAG
Aor51HI 73 AGCGCT AGC^GCT
AseI 2116 ATTAAT AT^TAAT
AsnI 2116 ATTAAT AT^TAAT
Asp718I 2727 GGTACC G^GTACC
BbeI 2369 GGCGCC GGCGC^C
BbvCI 3214 GCTGAGG CCTCAGC(-5/-2)
BciVI 2279 GTATCC GTATCC(6/5)
BfrI 2314 CTTAAG C^TTAAG
BsaAI 2897 TACGTG [CT]AC^GT[GA]
BspDI 677 ATCGAT AT^CGAT
BssHII 128 GCGCGC G^CGCGC
BstBI 2961 TTCGAA TT^CGAA
BstXI 1909 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 677 ATCGAT AT^CGAT
DraI 3713 TTTAAA TTT^AAA
DrdI 140 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 73 AGCGCT AGC^GCT
EcoRI 2067 GAATTC G^AATTC
HpaI 3887 GTTAAC GTT^AAC
KasI 2369 GGCGCC G^GCGCC
KpnI 2727 GGTACC GGTAC^C
KspI 194 CCGCGG CCGC^GG
MfeI 3095 CAATTG C^AATTG
MunI 3095 CAATTG C^AATTG
NarI 2369 GGCGCC GG^CGCC
NspV 2961 TTCGAA TT^CGAA
PaeR7I 336 CTCGAG C^TCGAG
PshBI 2116 ATTAAT AT^TAAT
SacII 194 CCGCGG CCGC^GG
SalI 2413 GTCGAC G^TCGAC
SapI 3744 GCTCTTC GCTCTTC(1/4)
SfoI 2369 GGCGCC GGC^GCC
SfuI 2961 TTCGAA TT^CGAA
StuI 1068 AGGCCT AGG^CCT
SwaI 3712 ATTTAAAT ATTT^AAAT
XhoI 336 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3419 GACGTC GACGT^C
3848 GACGTC
AccI 149 GTCTAC GT^[AC][GT]AC
2413 GTCGAC
AccIII 2223 TCCGGA T^CCGGA
2988 TCCGGA
AcsI 2067 GAATTC [GA]^AATT[CT]
3710 AAATTT
AflIII 2898 ACGTGT A^C[GA][CT]GT
3975 ACGTGT
Alw44I 408 GTGCAC G^TGCAC
3305 GTGCAC
ApaLI 408 GTGCAC G^TGCAC
3305 GTGCAC
ApoI 2067 GAATTC [GA]^AATT[CT]
3710 AAATTT
BalI 1050 TGGCCA TGG^CCA
1602 TGGCCA
BamHI 331 GGATCC G^GATCC
1403 GGATCC
BglI 392 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2361 GCCTGGGAGGC
BlpI 28 GCTGAGC GC^T[ACGT]AGC
4030 GCTAAGC
Bpu1102I 28 GCTGAGC GC^T[ACGT]AGC
4030 GCTAAGC
BsaBI 1108 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3391 GATGAATATC
BseAI 2223 TCCGGA T^CCGGA
2988 TCCGGA
BsgI 1340 CTGCAC GTGCAG(16/14)
1997 GTGCAG
Bsp1407I 3778 TGTACA T^GTACA
3899 TGTACA
BspEI 2223 TCCGGA T^CCGGA
2988 TCCGGA
BsrGI 3778 TGTACA T^GTACA
3899 TGTACA
BssSI 632 CACGAG CACGAG(-5/-1)
1589 CACGAG
CelII 28 GCTGAGC GC^T[ACGT]AGC
4030 GCTAAGC
HindIII 949 AAGCTT A^AGCTT
1055 AAGCTT
MamI 1108 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3391 GATGAATATC
MluNI 1050 TGGCCA TGG^CCA
1602 TGGCCA
MroI 2223 TCCGGA T^CCGGA
2988 TCCGGA
MscI 1050 TGGCCA TGG^CCA
1602 TGGCCA
NdeI 270 CATATG CA^TATG
365 CATATG
NspI 478 GCATGC [GA]CATG^[CT]
1371 GCATGC
PflMI 177 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
278 CCACAGGTTGG
SexAI 381 ACCTGGT A^CC[AT]GGT
426 ACCAGGT
SmaI 591 CCCGGG CCC^GGG
1236 CCCGGG
SphI 478 GCATGC GCATG^C
1371 GCATGC
SspBI 3778 TGTACA T^GTACA
3899 TGTACA
SspI 2113 AATATT AAT^ATT
3904 AATATT
Van91I 177 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
278 CCACAGGTTGG
XcmI 1320 CCACCCGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4141 CCATCAATAAAGTGG
XmaCI 591 CCCGGG C^CCGGG
1236 CCCGGG
XmaI 591 CCCGGG C^CCGGG
1236 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII