##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BalI BbrPI BciVI
BfrI BglII BlpI Bpu1102I BsaAI
BsiWI BspDI BspHI BstBI CelII
ClaI CpoI DraIII DrdI Eco47III
EcoNI EcoT22I FseI FspI HpaI
KpnI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 2078 GACGTC GACGT^C
AccIII 3392 TCCGGA T^CCGGA
AflIII 2789 ACATGT A^C[GA][CT]GT
AgeI 3565 ACCGGT A^CCGGT
AhdI 3343 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2412 GGGCCC GGGCC^C
AspEI 3343 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 179 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1390 CCTAGG C^CTAGG
BcgI 744 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2799 TGATCA T^GATCA
BglI 1545 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1390 CCTAGG C^CTAGG
BsaBI 1020 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 638 GAGACC GGTCTC(1/5)
BseAI 3392 TCCGGA T^CCGGA
Bsp1407I 3702 TGTACA T^GTACA
BspEI 3392 TCCGGA T^CCGGA
BspLU11I 2789 ACATGT A^CATGT
BsrBI 3691 CCGCTC CCGCTC(-3/-3)
BsrDI 3018 CATTGC GCAATG(2/0)
BsrGI 3702 TGTACA T^GTACA
BssHII 54 GCGCGC G^CGCGC
BssSI 621 CACGAG CACGAG(-5/-1)
Bst1107I 903 GTATAC GTA^TAC
BstAPI 1599 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 958 CCATTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 903 GTATAC GTA^TAC
DraI 562 TTTAAA TTT^AAA
EagI 3678 CGGCCG C^GGCCG
Eam1105I 3343 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3678 CGGCCG C^GGCCG
Eco52I 3678 CGGCCG C^GGCCG
EcoRV 1171 GATATC GAT^ATC
FbaI 2799 TGATCA T^GATCA
HincII 2875 GTCAAC GT[CT]^[GA]AC
HindII 2875 GTCAAC GT[CT]^[GA]AC
HindIII 2780 AAGCTT A^AGCTT
KspI 34 CCGCGG CCGC^GG
MamI 1020 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3392 TCCGGA T^CCGGA
NaeI 117 GCCGGC GCC^GGC
NgoMIV 117 GCCGGC G^CCGGC
PaeR7I 2453 CTCGAG C^TCGAG
PflFI 179 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3565 ACCGGT A^CCGGT
PshAI 829 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 724 CAGCTG CAG^CTG
SacI 3241 GAGCTC GAGCT^C
SacII 34 CCGCGG CCGC^GG
SmaI 63 CCCGGG CCC^GGG
SspBI 3702 TGTACA T^GTACA
Tth111I 179 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2453 CTCGAG C^TCGAG
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 305 GTCTAC GT^[AC][GT]AC
903 GTATAC
AlwNI 414 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3151 CAGTGCCTG
AocI 108 CCTGAGG CC^T[ACGT]AGG
2944 CCTGAGG
BamHI 2030 GGATCC G^GATCC
2271 GGATCC
BbeI 188 GGCGCC GGCGC^C
710 GGCGCC
BbsI 2521 GAAGAC GAAGAC(2/6)
1907 GTCTTC
BbvCI 2034 CCTCAGC CCTCAGC(-5/-2)
738 GCTGAGG
BpuAI 2521 GAAGAC GAAGAC(2/6)
1907 GTCTTC
BsaWI 3392 TCCGGA [AT]^CCGG[AT]
3565 ACCGGT
BsiEI 26 CGGTCG CG[GA][CT]^CG
3678 CGGCCG
BsmBI 241 CGTCTC CGTCTC(1/5)
2076 GAGACG
BsmI 2533 GAATGC GAATGC(1/-1)
2841 GAATGC
BspMI 2936 ACCTGC ACCTGC(4/8)
3202 ACCTGC
BstEII 1107 GGTGACC G^GT[ACGT]ACC
2856 GGTCACC
BstPI 1107 GGTGACC G^GT[ACGT]ACC
2856 GGTCACC
BstYI 2030 GGATCC [GA]^GATC[CT]
2271 GGATCC
Bsu36I 108 CCTGAGG CC^T[ACGT]AGG
2944 CCTGAGG
Eco81I 108 CCTGAGG CC^T[ACGT]AGG
2944 CCTGAGG
EcoO65I 1107 GGTGACC G^GT[ACGT]ACC
2856 GGTCACC
EcoRI 1335 GAATTC G^AATTC
3007 GAATTC
HaeII 188 GGCGCC [GA]GCGC^[CT]
710 GGCGCC
KasI 188 GGCGCC G^GCGCC
710 GGCGCC
MaeII 372 ACGT A^CGT
2079 ACGT
MflI 2030 GGATCC [GA]^GATC[CT]
2271 GGATCC
NarI 188 GGCGCC GG^CGCC
710 GGCGCC
NspI 2175 GCATGT [GA]CATG^[CT]
2789 ACATGT
PflMI 413 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3250 CCAGAGAGTGG
PstI 2318 CTGCAG CTGCA^G
3509 CTGCAG
SapI 2374 GAAGAGC GCTCTTC(1/4)
3136 GAAGAGC
SfoI 188 GGCGCC GGC^GCC
710 GGCGCC
SpeI 1502 ACTAGT A^CTAGT
2101 ACTAGT
StuI 1190 AGGCCT AGG^CCT
1393 AGGCCT
TaqI 636 TCGA T^CGA
2454 TCGA
TfiI 750 GAATC G^A[AT]TC
871 GATTC
TthHB8I 636 TCGA T^CGA
2454 TCGA
Van91I 413 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3250 CCAGAGAGTGG
XhoII 2030 GGATCC [GA]^GATC[CT]
2271 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI