##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI BspDI BspEI
BsrFI BssSI Bst1107I BstAPI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 712 GTCTAC GT^[AC][GT]AC
AclI 3044 AACGTT AA^CGTT
AlwNI 2546 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2927 CCTTAGG CC^T[ACGT]AGG
ApaI 3142 GGGCCC GGGCC^C
Asp700I 4246 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 136 CTCGGG C^[CT]CG[GA]G
BcgI 95 CGAAGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 86 GGATAC GTATCC(6/5)
BpmI 221 CTGGAG CTGGAG(16/14)
BsaWI 209 ACCGGA [AT]^CCGG[AT]
BseRI 316 GAGGAG GAGGAG(10/8)
BsiEI 427 CGGTCG CG[GA][CT]^CG
BsmBI 1188 GAGACG CGTCTC(1/5)
BsoBI 136 CTCGGG C^[CT]CG[GA]G
BspHI 33 TCATGA T^CATGA
BsrBI 201 GAGCGG CCGCTC(-3/-3)
BssHII 57 GCGCGC G^CGCGC
BstBI 3129 TTCGAA TT^CGAA
Bsu36I 2927 CCTTAGG CC^T[ACGT]AGG
DrdI 147 GACCCAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2927 CCTTAGG CC^T[ACGT]AGG
EcoRI 1368 GAATTC G^AATTC
EcoRV 833 GATATC GAT^ATC
HaeII 38 AGCGCC [GA]GCGC^[CT]
HapII 210 CCGG C^CGG
HincII 3824 GTTAAC GT[CT]^[GA]AC
HindII 3824 GTTAAC GT[CT]^[GA]AC
HpaI 3824 GTTAAC GTT^AAC
HpaII 210 CCGG C^CGG
MaeII 3045 ACGT A^CGT
MfeI 1385 CAATTG C^AATTG
MspI 210 CCGG C^CGG
MunI 1385 CAATTG C^AATTG
NcoI 3015 CCATGG C^CATGG
NspV 3129 TTCGAA TT^CGAA
PshAI 1606 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3044 AACGTT AA^CGTT
RcaI 33 TCATGA T^CATGA
SacI 1874 GAGCTC GAGCT^C
SfuI 3129 TTCGAA TT^CGAA
SmlI 66 CTTGAG C^T[CT][GA]AG
StuI 3401 AGGCCT AGG^CCT
XbaI 2840 TCTAGA T^CTAGA
XmnI 4246 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 213 GACGCC G[GA]^CG[CT]C
375 GACGCC
BanII 1874 GAGCTC G[GA]GC[CT]^C
3142 GGGCCC
BbvCI 1257 CCTCAGC CCTCAGC(-5/-2)
2197 CCTCAGC
BglI 415 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
658 GCCAGCTTGGC
BsaHI 213 GACGCC G[GA]^CG[CT]C
375 GACGCC
BsgI 682 CTGCAC GTGCAG(16/14)
2617 GTGCAG
BsmI 2202 GCATTC GAATGC(1/-1)
3647 GCATTC
Bsp1407I 2964 TGTACA T^GTACA
4142 TGTACA
BsrDI 3053 CATTGC GCAATG(2/0)
3109 GCAATG
BsrGI 2964 TGTACA T^GTACA
4142 TGTACA
BstXI 3286 CCAACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4266 CCACATTTCTGG
BstYI 305 GGATCT [GA]^GATC[CT]
472 GGATCT
DsaI 788 CCGTGG C^C[GA][CT]GG
3015 CCATGG
EarI 96 GAAGAG CTCTTC(1/4)
2980 GAAGAG
Hin1I 213 GACGCC G[GA]^CG[CT]C
375 GACGCC
HindIII 1981 AAGCTT A^AGCTT
2763 AAGCTT
Ksp632I 96 GAAGAG CTCTTC(1/4)
2980 GAAGAG
MflI 305 GGATCT [GA]^GATC[CT]
472 GGATCT
MspA1I 142 CAGCTG C[AC]G^C[GT]G
2266 CAGCTG
PflMI 2662 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3214 CCACATGTTGG
PpuMI 510 AGGACCC [GA]G^G[AT]CC[CT]
3078 AGGTCCT
PvuII 142 CAGCTG CAG^CTG
2266 CAGCTG
SfaNI 2068 GATGC GCATC(5/9)
2578 GATGC
SspBI 2964 TGTACA T^GTACA
4142 TGTACA
SwaI 2119 ATTTAAAT ATTT^AAAT
3495 ATTTAAAT
Van91I 2662 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3214 CCACATGTTGG
XhoII 305 GGATCT [GA]^GATC[CT]
472 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaII BalI BanI BbsI
BbvI BclI BfaI BmyI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MvaI MvnI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfcI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI