##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI BbeI BcgI BclI BfrI
BglII BsmI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI HpaI KasI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SfoI SfuI SnaBI SphI SwaI
Tru9I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 7185 GACGTC GACGT^C
Acc65I 3562 GGTACC G^GTACC
AccIII 6778 TCCGGA T^CCGGA
AgeI 925 ACCGGT A^CCGGT
AhdI 5870 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3562 GGTACC G^GTACC
AspEI 5870 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 7214 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 7294 TGCGCA TGC^GCA
AvrII 1176 CCTAGG C^CTAGG
BamHI 3785 GGATCC G^GATCC
BbsI 4249 GAAGAC GAAGAC(2/6)
BciVI 7514 GGATAC GTATCC(6/5)
BlnI 1176 CCTAGG C^CTAGG
BpuAI 4249 GAAGAC GAAGAC(2/6)
BsaBI 5570 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 6778 TCCGGA T^CCGGA
BsiEI 5239 CGACCG CG[GA][CT]^CG
BsiWI 5873 CGTACG C^GTACG
BspEI 6778 TCCGGA T^CCGGA
BspHI 4715 TCATGA T^CATGA
BspLU11I 7502 ACATGT A^CATGT
BssHII 7555 GCGCGC G^CGCGC
Eam1105I 5870 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 7294 TGCGCA TGC^GCA
HincII 929 GTCAAC GT[CT]^[GA]AC
HindII 929 GTCAAC GT[CT]^[GA]AC
HindIII 7394 AAGCTT A^AGCTT
KpnI 3562 GGTACC GGTAC^C
KspI 6377 CCGCGG CCGC^GG
MamI 5570 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 6778 TCCGGA T^CCGGA
PaeR7I 3289 CTCGAG C^TCGAG
PflFI 7214 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 925 ACCGGT A^CCGGT
RcaI 4715 TCATGA T^CATGA
SacII 6377 CCGCGG CCGC^GG
SbfI 4982 CCTGCAGG CCTGCA^GG
ScaI 6841 AGTACT AGT^ACT
SexAI 801 ACCAGGT A^CC[AT]GGT
SgrAI 848 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3798 ACTAGT A^CTAGT
Sse8387I 4982 CCTGCAGG CCTGCA^GG
SspI 5677 AATATT AAT^ATT
Tth111I 7214 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3289 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Aor51HI 6160 AGCGCT AGC^GCT
7159 AGCGCT
Asp700I 1282 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2977 GAACCTCTTC
BsaWI 925 ACCGGT [AT]^CCGG[AT]
6778 TCCGGA
Bsp1407I 2760 TGTACA T^GTACA
7505 TGTACA
BsrDI 7001 CATTGC GCAATG(2/0)
7021 GCAATG
BsrGI 2760 TGTACA T^GTACA
7505 TGTACA
BstEII 4280 GGTGACC G^GT[ACGT]ACC
5456 GGTGACC
BstPI 4280 GGTGACC G^GT[ACGT]ACC
5456 GGTGACC
Eco47III 6160 AGCGCT AGC^GCT
7159 AGCGCT
EcoO65I 4280 GGTGACC G^GT[ACGT]ACC
5456 GGTGACC
EcoRI 1418 GAATTC G^AATTC
2897 GAATTC
NspI 6546 GCATGT [GA]CATG^[CT]
7502 ACATGT
SapI 6720 GAAGAGC GCTCTTC(1/4)
6438 GCTCTTC
SspBI 2760 TGTACA T^GTACA
7505 TGTACA
XmnI 1282 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2977 GAACCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI