################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AccI AclI AflII AscI AseI AsnI Asp700I AspI AviII BamHI BciVI BfrI BglII BsaBI BsmI BspDI BspHI BspLU11I BsrDI Bst1107I BstAPI BstBI BstZ17I ClaI DraIII DrdI EcoRI EcoT22I FseI FspI HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI SapI ScaI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3263 GACGTC GACGT^C AccIII 2017 TCCGGA T^CCGGA AcsI 3162 AAATTC [GA]^AATT[CT] AflIII 2386 ACGTGT A^C[GA][CT]GT AgeI 1018 ACCGGT A^CCGGT AhdI 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 273 GTGCAC G^TGCAC ApaLI 273 GTGCAC G^TGCAC ApoI 3162 AAATTC [GA]^AATT[CT] AspEI 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 159 CCTAGG C^CTAGG BfaI 160 CTAG C^TAG BlnI 159 CCTAGG C^CTAGG BlpI 2249 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2249 GCTGAGC GC^T[ACGT]AGC BseAI 2017 TCCGGA T^CCGGA BsgI 29 GTGCAG GTGCAG(16/14) BsiWI 3561 CGTACG C^GTACG BsmBI 971 GAGACG CGTCTC(1/5) Bsp1407I 202 TGTACA T^GTACA BspEI 2017 TCCGGA T^CCGGA BsrGI 202 TGTACA T^GTACA BssHII 2235 GCGCGC G^CGCGC BstEII 3290 GGTGACC G^GT[ACGT]ACC BstPI 3290 GGTGACC G^GT[ACGT]ACC BstYI 3131 GGATCT [GA]^GATC[CT] CelII 2249 GCTGAGC GC^T[ACGT]AGC CpoI 1350 CGGACCG CG^G[AT]CCG DraI 3496 TTTAAA TTT^AAA Eam1105I 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3290 GGTGACC G^GT[ACGT]ACC EcoRV 2127 GATATC GAT^ATC HincII 3602 GTCAAC GT[CT]^[GA]AC HindII 3602 GTCAAC GT[CT]^[GA]AC HindIII 2538 AAGCTT A^AGCTT KspI 2939 CCGCGG CCGC^GG MaeI 160 CTAG C^TAG MflI 3131 GGATCT [GA]^GATC[CT] MroI 2017 TCCGGA T^CCGGA PinAI 1018 ACCGGT A^CCGGT RsrII 1350 CGGACCG CG^G[AT]CCG SacI 1108 GAGCTC GAGCT^C SacII 2939 CCGCGG CCGC^GG SexAI 946 ACCAGGT A^CC[AT]GGT SphI 2838 GCATGC GCATG^C SspBI 202 TGTACA T^GTACA TaqI 3070 TCGA T^CGA Tsp509I 3163 AATT ^AATT TthHB8I 3070 TCGA T^CGA XhoII 3131 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 331 GGTACC G^GTACC 950 GGTACC Aor51HI 1921 AGCGCT AGC^GCT 2971 AGCGCT Asp718I 331 GGTACC G^GTACC 950 GGTACC BalI 1672 TGGCCA TGG^CCA 1906 TGGCCA BbrPI 2385 CACGTG CAC^GTG 2653 CACGTG BbvCI 700 CCTCAGC CCTCAGC(-5/-2) 2433 GCTGAGG BcgI 187 CGAGGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1103 GCACCGAGCTCG BclI 2527 TGATCA T^GATCA 2992 TGATCA BsaAI 2385 CACGTG [CT]AC^GT[GA] 2653 CACGTG BssSI 2860 CACGAG CACGAG(-5/-1) 2349 CTCGTG BstXI 901 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2564 CCATAGCTCTGG Eco47III 1921 AGCGCT AGC^GCT 2971 AGCGCT EcoNI 284 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 667 CCTCCACCAGG FbaI 2527 TGATCA T^GATCA 2992 TGATCA KpnI 331 GGTACC GGTAC^C 950 GGTACC MluNI 1672 TGGCCA TGG^CCA 1906 TGGCCA MscI 1672 TGGCCA TGG^CCA 1906 TGGCCA MslI 680 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3144 CACTACTATG NcoI 346 CCATGG C^CATGG 985 CCATGG PflMI 141 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 876 CCAGAGCCTGG PmaCI 2385 CACGTG CAC^GTG 2653 CACGTG PmlI 2385 CACGTG CAC^GTG 2653 CACGTG SbfI 1286 CCTGCAGG CCTGCA^GG 2960 CCTGCAGG Sse8387I 1286 CCTGCAGG CCTGCA^GG 2960 CCTGCAGG StuI 922 AGGCCT AGG^CCT 1447 AGGCCT Van91I 141 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 876 CCAGAGCCTGG XcmI 215 CCAGGACGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 673 CCAGGGCCACTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I TspRI XmaCI XmaI