##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccI AclI AflII AscI AseI
AsnI Asp700I AspI AviII BamHI
BciVI BfrI BglII BsaBI BsmI
BspDI BspHI BspLU11I BsrDI Bst1107I
BstAPI BstBI BstZ17I ClaI DraIII
DrdI EcoRI EcoT22I FseI FspI
HpaI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SalI SapI ScaI SfuI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3263 GACGTC GACGT^C
AccIII 2017 TCCGGA T^CCGGA
AcsI 3162 AAATTC [GA]^AATT[CT]
AflIII 2386 ACGTGT A^C[GA][CT]GT
AgeI 1018 ACCGGT A^CCGGT
AhdI 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 273 GTGCAC G^TGCAC
ApaLI 273 GTGCAC G^TGCAC
ApoI 3162 AAATTC [GA]^AATT[CT]
AspEI 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 159 CCTAGG C^CTAGG
BfaI 160 CTAG C^TAG
BlnI 159 CCTAGG C^CTAGG
BlpI 2249 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2249 GCTGAGC GC^T[ACGT]AGC
BseAI 2017 TCCGGA T^CCGGA
BsgI 29 GTGCAG GTGCAG(16/14)
BsiWI 3561 CGTACG C^GTACG
BsmBI 971 GAGACG CGTCTC(1/5)
Bsp1407I 202 TGTACA T^GTACA
BspEI 2017 TCCGGA T^CCGGA
BsrGI 202 TGTACA T^GTACA
BssHII 2235 GCGCGC G^CGCGC
BstEII 3290 GGTGACC G^GT[ACGT]ACC
BstPI 3290 GGTGACC G^GT[ACGT]ACC
BstYI 3131 GGATCT [GA]^GATC[CT]
CelII 2249 GCTGAGC GC^T[ACGT]AGC
CpoI 1350 CGGACCG CG^G[AT]CCG
DraI 3496 TTTAAA TTT^AAA
Eam1105I 3468 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3290 GGTGACC G^GT[ACGT]ACC
EcoRV 2127 GATATC GAT^ATC
HincII 3602 GTCAAC GT[CT]^[GA]AC
HindII 3602 GTCAAC GT[CT]^[GA]AC
HindIII 2538 AAGCTT A^AGCTT
KspI 2939 CCGCGG CCGC^GG
MaeI 160 CTAG C^TAG
MflI 3131 GGATCT [GA]^GATC[CT]
MroI 2017 TCCGGA T^CCGGA
PinAI 1018 ACCGGT A^CCGGT
RsrII 1350 CGGACCG CG^G[AT]CCG
SacI 1108 GAGCTC GAGCT^C
SacII 2939 CCGCGG CCGC^GG
SexAI 946 ACCAGGT A^CC[AT]GGT
SphI 2838 GCATGC GCATG^C
SspBI 202 TGTACA T^GTACA
TaqI 3070 TCGA T^CGA
Tsp509I 3163 AATT ^AATT
TthHB8I 3070 TCGA T^CGA
XhoII 3131 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 331 GGTACC G^GTACC
950 GGTACC
Aor51HI 1921 AGCGCT AGC^GCT
2971 AGCGCT
Asp718I 331 GGTACC G^GTACC
950 GGTACC
BalI 1672 TGGCCA TGG^CCA
1906 TGGCCA
BbrPI 2385 CACGTG CAC^GTG
2653 CACGTG
BbvCI 700 CCTCAGC CCTCAGC(-5/-2)
2433 GCTGAGG
BcgI 187 CGAGGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1103 GCACCGAGCTCG
BclI 2527 TGATCA T^GATCA
2992 TGATCA
BsaAI 2385 CACGTG [CT]AC^GT[GA]
2653 CACGTG
BssSI 2860 CACGAG CACGAG(-5/-1)
2349 CTCGTG
BstXI 901 CCAGCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2564 CCATAGCTCTGG
Eco47III 1921 AGCGCT AGC^GCT
2971 AGCGCT
EcoNI 284 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
667 CCTCCACCAGG
FbaI 2527 TGATCA T^GATCA
2992 TGATCA
KpnI 331 GGTACC GGTAC^C
950 GGTACC
MluNI 1672 TGGCCA TGG^CCA
1906 TGGCCA
MscI 1672 TGGCCA TGG^CCA
1906 TGGCCA
MslI 680 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3144 CACTACTATG
NcoI 346 CCATGG C^CATGG
985 CCATGG
PflMI 141 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
876 CCAGAGCCTGG
PmaCI 2385 CACGTG CAC^GTG
2653 CACGTG
PmlI 2385 CACGTG CAC^GTG
2653 CACGTG
SbfI 1286 CCTGCAGG CCTGCA^GG
2960 CCTGCAGG
Sse8387I 1286 CCTGCAGG CCTGCA^GG
2960 CCTGCAGG
StuI 922 AGGCCT AGG^CCT
1447 AGGCCT
Van91I 141 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
876 CCAGAGCCTGG
XcmI 215 CCAGGACGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
673 CCAGGGCCACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
StyI TfiI Tru9I TseI Tsp45I
TspRI XmaCI XmaI