##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
Alw44I ApaLI AscI Asp700I AspEI
AspI AviII BbrPI BciVI BclI
BfrI BglI BsaAI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FbaI FseI FspI
HindIII KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2560 GGTACC G^GTACC
AccI 283 GTCTAC GT^[AC][GT]AC
AccII 1293 CGCG CG^CG
AclI 3311 AACGTT AA^CGTT
AflIII 2171 ACACGT A^C[GA][CT]GT
AocI 69 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1336 AGCGCT AGC^GCT
ApaI 2100 GGGCCC GGGCC^C
Asp718I 2560 GGTACC G^GTACC
AspHI 2046 GAGCAC G[AT]GC[AT]^C
AvrII 2130 CCTAGG C^CTAGG
BalI 552 TGGCCA TGG^CCA
BamHI 572 GGATCC G^GATCC
BbvCI 971 CCTCAGC CCTCAGC(-5/-2)
BcgI 2043 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2330 AGATCT A^GATCT
BlnI 2130 CCTAGG C^CTAGG
BlpI 763 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 763 GCTCAGC GC^T[ACGT]AGC
BsaBI 1889 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1409 GAGACC GGTCTC(1/5)
BsaWI 308 ACCGGA [AT]^CCGG[AT]
BsiHKAI 2046 GAGCAC G[AT]GC[AT]^C
BspHI 3804 TCATGA T^CATGA
BspMI 444 ACCTGC ACCTGC(4/8)
BstUI 1293 CGCG CG^CG
Bsu36I 69 CCTTAGG CC^T[ACGT]AGG
CelII 763 GCTCAGC GC^T[ACGT]AGC
DrdI 1519 GACAATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 552 TGGCCA [CT]^GGCC[GA]
Eco47III 1336 AGCGCT AGC^GCT
Eco81I 69 CCTTAGG CC^T[ACGT]AGG
FauI 2084 CCCGC CCCGC(4/6)
KpnI 2560 GGTACC GGTAC^C
MamI 1889 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 552 TGGCCA TGG^CCA
MscI 552 TGGCCA TGG^CCA
MvnI 1293 CGCG CG^CG
PpuMI 582 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 307 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3311 AACGTT AA^CGTT
RcaI 3804 TCATGA T^CATGA
SapI 689 GCTCTTC GCTCTTC(1/4)
XcmI 1548 CCAAGCTATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AseI 1147 ATTAAT AT^TAAT
2308 ATTAAT
AsnI 1147 ATTAAT AT^TAAT
2308 ATTAAT
AvaI 1099 CTCGGG C^[CT]CG[GA]G
2041 CCCGAG
BanII 1025 GAGCCC G[GA]GC[CT]^C
2100 GGGCCC
BbsI 475 GAAGAC GAAGAC(2/6)
1465 GTCTTC
BpuAI 475 GAAGAC GAAGAC(2/6)
1465 GTCTTC
BsmBI 3083 CGTCTC CGTCTC(1/5)
1748 GAGACG
BsoBI 1099 CTCGGG C^[CT]CG[GA]G
2041 CCCGAG
BstAPI 640 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1534 GCACAAGCTGC
BstXI 464 CCAACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
866 CCAGCAACCTGG
DraII 582 GGGACCT [GA]G^G[ACGT]CC[CT]
2099 AGGGCCC
EarI 690 CTCTTC CTCTTC(1/4)
3013 CTCTTC
EcoO109I 582 GGGACCT [GA]G^G[ACGT]CC[CT]
2099 AGGGCCC
EcoT22I 421 ATGCAT ATGCA^T
661 ATGCAT
HincII 2117 GTTAAC GT[CT]^[GA]AC
2548 GTTAAC
HindII 2117 GTTAAC GT[CT]^[GA]AC
2548 GTTAAC
HpaI 2117 GTTAAC GTT^AAC
2548 GTTAAC
Ksp632I 690 CTCTTC CTCTTC(1/4)
3013 CTCTTC
NsiI 421 ATGCAT ATGCA^T
661 ATGCAT
NspI 3069 GCATGT [GA]CATG^[CT]
3193 GCATGT
PshBI 1147 ATTAAT AT^TAAT
2308 ATTAAT
PvuII 956 CAGCTG CAG^CTG
1594 CAGCTG
SmlI 376 CTTGAG C^T[CT][GA]AG
1975 CTTGAG
TaqI 505 TCGA T^CGA
600 TCGA
TthHB8I 505 TCGA T^CGA
600 TCGA
XbaI 2609 TCTAGA T^CTAGA
3490 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaII BanI
BbeI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EcoNI EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SspBI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII