##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI ApaI AscI AseI
AsnI Asp718I AvrII BamHI BbeI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrDI BssHII
BssSI BstBI BstYI CelII ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRV FseI Hin1I HindIII KasI
KpnI KspI MamI MfeI MflI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XbaI XhoII
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflIII 657 ACATGT A^C[GA][CT]GT
Alw44I 1497 GTGCAC G^TGCAC
Aor51HI 3071 AGCGCT AGC^GCT
ApaLI 1497 GTGCAC G^TGCAC
Asp700I 860 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3273 TGCGCA TGC^GCA
BbrPI 3311 CACGTG CAC^GTG
BciVI 637 GGATAC GTATCC(6/5)
BsaAI 3311 CACGTG [CT]AC^GT[GA]
BsmBI 2064 GAGACG CGTCTC(1/5)
BspHI 2124 TCATGA T^CATGA
BspLU11I 657 ACATGT A^CATGT
BsrFI 3110 GCCGGC [GA]^CCGG[CT]
Bst1107I 661 GTATAC GTA^TAC
BstZ17I 661 GTATAC GTA^TAC
Cfr10I 3110 GCCGGC [GA]^CCGG[CT]
DraI 1303 TTTAAA TTT^AAA
EarI 2510 CTCTTC CTCTTC(1/4)
Eco47III 3071 AGCGCT AGC^GCT
EcoNI 3736 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1606 ATGCAT ATGCA^T
FspI 3273 TGCGCA TGC^GCA
HgaI 2066 GACGC GACGC(5/10)
HpaI 897 GTTAAC GTT^AAC
Ksp632I 2510 CTCTTC CTCTTC(1/4)
NaeI 3110 GCCGGC GCC^GGC
NgoMIV 3110 GCCGGC G^CCGGC
NsiI 1606 ATGCAT ATGCA^T
NspI 657 ACATGT [GA]CATG^[CT]
PaeR7I 3305 CTCGAG C^TCGAG
PmaCI 3311 CACGTG CAC^GTG
PmlI 3311 CACGTG CAC^GTG
PpuMI 101 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 2124 TCATGA T^CATGA
SacI 1934 GAGCTC GAGCT^C
ScaI 3429 AGTACT AGT^ACT
SmaI 3632 CCCGGG CCC^GGG
StuI 3744 AGGCCT AGG^CCT
XhoI 3305 CTCGAG C^TCGAG
XmaCI 3632 CCCGGG C^CCGGG
XmaI 3632 CCCGGG C^CCGGG
XmnI 860 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 661 GTATAC GT^[AC][GT]AC
1576 GTAGAC
AhdI 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4056 GACTCTCTGTC
AocI 1516 CCTCAGG CC^T[ACGT]AGG
3063 CCTCAGG
AspEI 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4056 GACTCTCTGTC
AspI 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACAGAGTC
BbsI 995 GAAGAC GAAGAC(2/6)
3596 GTCTTC
BcgI 378 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2745 CGATCCTCTTGC
BpuAI 995 GAAGAC GAAGAC(2/6)
3596 GTCTTC
Bsp1407I 623 TGTACA T^GTACA
1553 TGTACA
BspMI 2252 ACCTGC ACCTGC(4/8)
2270 ACCTGC
BsrGI 623 TGTACA T^GTACA
1553 TGTACA
BstEII 3577 GGTGACC G^GT[ACGT]ACC
3616 GGTCACC
BstPI 3577 GGTGACC G^GT[ACGT]ACC
3616 GGTCACC
Bsu36I 1516 CCTCAGG CC^T[ACGT]AGG
3063 CCTCAGG
DraII 101 GGGACCC [GA]G^G[ACGT]CC[CT]
3854 AGGGCCT
DraIII 86 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2134 CACGCTGTG
Eam1105I 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4056 GACTCTCTGTC
Eco81I 1516 CCTCAGG CC^T[ACGT]AGG
3063 CCTCAGG
EcoO109I 101 GGGACCC [GA]G^G[ACGT]CC[CT]
3854 AGGGCCT
EcoO65I 3577 GGTGACC G^GT[ACGT]ACC
3616 GGTCACC
EcoRI 3 GAATTC G^AATTC
1317 GAATTC
HaeII 3071 AGCGCT [GA]GCGC^[CT]
3359 AGCGCC
HincII 897 GTTAAC GT[CT]^[GA]AC
1187 GTTGAC
HindII 897 GTTAAC GT[CT]^[GA]AC
1187 GTTGAC
NcoI 3178 CCATGG C^CATGG
3914 CCATGG
PflFI 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACAGAGTC
SspBI 623 TGTACA T^GTACA
1553 TGTACA
SspI 1041 AATATT AAT^ATT
2923 AATATT
Tth111I 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1726 GACAGAGTC
XcmI 2488 CCATTATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3537 CCACGGGGCGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BclI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I