################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI ApaI AscI AseI AsnI Asp718I AvrII BamHI BbeI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrDI BssHII BssSI BstBI BstYI CelII ClaI CpoI DrdI EagI EclXI Eco52I EcoRV FseI Hin1I HindIII KasI KpnI KspI MamI MfeI MflI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoII ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 657 ACATGT A^C[GA][CT]GT Alw44I 1497 GTGCAC G^TGCAC Aor51HI 3071 AGCGCT AGC^GCT ApaLI 1497 GTGCAC G^TGCAC Asp700I 860 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3273 TGCGCA TGC^GCA BbrPI 3311 CACGTG CAC^GTG BciVI 637 GGATAC GTATCC(6/5) BsaAI 3311 CACGTG [CT]AC^GT[GA] BsmBI 2064 GAGACG CGTCTC(1/5) BspHI 2124 TCATGA T^CATGA BspLU11I 657 ACATGT A^CATGT BsrFI 3110 GCCGGC [GA]^CCGG[CT] Bst1107I 661 GTATAC GTA^TAC BstZ17I 661 GTATAC GTA^TAC Cfr10I 3110 GCCGGC [GA]^CCGG[CT] DraI 1303 TTTAAA TTT^AAA EarI 2510 CTCTTC CTCTTC(1/4) Eco47III 3071 AGCGCT AGC^GCT EcoNI 3736 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1606 ATGCAT ATGCA^T FspI 3273 TGCGCA TGC^GCA HgaI 2066 GACGC GACGC(5/10) HpaI 897 GTTAAC GTT^AAC Ksp632I 2510 CTCTTC CTCTTC(1/4) NaeI 3110 GCCGGC GCC^GGC NgoMIV 3110 GCCGGC G^CCGGC NsiI 1606 ATGCAT ATGCA^T NspI 657 ACATGT [GA]CATG^[CT] PaeR7I 3305 CTCGAG C^TCGAG PmaCI 3311 CACGTG CAC^GTG PmlI 3311 CACGTG CAC^GTG PpuMI 101 GGGACCC [GA]G^G[AT]CC[CT] RcaI 2124 TCATGA T^CATGA SacI 1934 GAGCTC GAGCT^C ScaI 3429 AGTACT AGT^ACT SmaI 3632 CCCGGG CCC^GGG StuI 3744 AGGCCT AGG^CCT XhoI 3305 CTCGAG C^TCGAG XmaCI 3632 CCCGGG C^CCGGG XmaI 3632 CCCGGG C^CCGGG XmnI 860 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 661 GTATAC GT^[AC][GT]AC 1576 GTAGAC AhdI 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4056 GACTCTCTGTC AocI 1516 CCTCAGG CC^T[ACGT]AGG 3063 CCTCAGG AspEI 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4056 GACTCTCTGTC AspI 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1726 GACAGAGTC BbsI 995 GAAGAC GAAGAC(2/6) 3596 GTCTTC BcgI 378 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2745 CGATCCTCTTGC BpuAI 995 GAAGAC GAAGAC(2/6) 3596 GTCTTC Bsp1407I 623 TGTACA T^GTACA 1553 TGTACA BspMI 2252 ACCTGC ACCTGC(4/8) 2270 ACCTGC BsrGI 623 TGTACA T^GTACA 1553 TGTACA BstEII 3577 GGTGACC G^GT[ACGT]ACC 3616 GGTCACC BstPI 3577 GGTGACC G^GT[ACGT]ACC 3616 GGTCACC Bsu36I 1516 CCTCAGG CC^T[ACGT]AGG 3063 CCTCAGG DraII 101 GGGACCC [GA]G^G[ACGT]CC[CT] 3854 AGGGCCT DraIII 86 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2134 CACGCTGTG Eam1105I 1198 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4056 GACTCTCTGTC Eco81I 1516 CCTCAGG CC^T[ACGT]AGG 3063 CCTCAGG EcoO109I 101 GGGACCC [GA]G^G[ACGT]CC[CT] 3854 AGGGCCT EcoO65I 3577 GGTGACC G^GT[ACGT]ACC 3616 GGTCACC EcoRI 3 GAATTC G^AATTC 1317 GAATTC HaeII 3071 AGCGCT [GA]GCGC^[CT] 3359 AGCGCC HincII 897 GTTAAC GT[CT]^[GA]AC 1187 GTTGAC HindII 897 GTTAAC GT[CT]^[GA]AC 1187 GTTGAC NcoI 3178 CCATGG C^CATGG 3914 CCATGG PflFI 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1726 GACAGAGTC SspBI 623 TGTACA T^GTACA 1553 TGTACA SspI 1041 AATATT AAT^ATT 2923 AATATT Tth111I 295 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1726 GACAGAGTC XcmI 2488 CCATTATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3537 CCACGGGGCGGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BclI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I