##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspI AviII AvrII
BcgI BciVI BfrI BglI BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrFI BssHII BstEII
BstPI Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
FauI FseI FspI KspI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SpeI Sse8387I SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 658 GGTACC G^GTACC
AcyI 55 GGCGCC G[GA]^CG[CT]C
AhdI 2693 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 924 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 658 GGTACC G^GTACC
AspEI 2693 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 91 GGATCC G^GATCC
BbeI 55 GGCGCC GGCGC^C
BbvCI 2551 CCTCAGC CCTCAGC(-5/-2)
BsaBI 3046 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 55 GGCGCC G[GA]^CG[CT]C
BsaI 2934 GGTCTC GGTCTC(1/5)
BsgI 2496 CTGCAC GTGCAG(16/14)
BspMI 2190 ACCTGC ACCTGC(4/8)
BsrBI 15 CCGCTC CCGCTC(-3/-3)
BsrDI 2397 CATTGC GCAATG(2/0)
Bst1107I 4474 GTATAC GTA^TAC
BstAPI 535 GCAAAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 131 TTCGAA TT^CGAA
BstZ17I 4474 GTATAC GTA^TAC
CfoI 56 GCGC GCG^C
Eam1105I 2693 GACATCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3593 GATATC GAT^ATC
HaeII 55 GGCGCC [GA]GCGC^[CT]
HhaI 56 GCGC GCG^C
Hin1I 55 GGCGCC G[GA]^CG[CT]C
HinP1I 56 GCGC G^CGC
HpaI 30 GTTAAC GTT^AAC
KasI 55 GGCGCC G^GCGCC
KpnI 658 GGTACC GGTAC^C
MamI 3046 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2493 CAGCTG C[AC]G^C[GT]G
NarI 55 GGCGCC GG^CGCC
NspV 131 TTCGAA TT^CGAA
PvuII 2493 CAGCTG CAG^CTG
SacI 3600 GAGCTC GAGCT^C
ScaI 1395 AGTACT AGT^ACT
SfoI 55 GGCGCC GGC^GCC
SfuI 131 TTCGAA TT^CGAA
SmlI 417 CTCAAG C^T[CT][GA]AG
SnaBI 4320 TACGTA TAC^GTA
SphI 2590 GCATGC GCATG^C
StuI 3479 AGGCCT AGG^CCT
XcmI 2907 CCACTTGGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 21 CGCG CG^CG
793 CGCG
AflIII 218 ACATGT A^C[GA][CT]GT
3536 ACATGT
AspHI 2996 GTGCTC G[AT]GC[AT]^C
3600 GAGCTC
AvaI 782 CTCGGG C^[CT]CG[GA]G
830 CCCGAG
BalI 1802 TGGCCA TGG^CCA
4009 TGGCCA
BbrPI 1904 CACGTG CAC^GTG
3974 CACGTG
BbsI 2769 GAAGAC GAAGAC(2/6)
1898 GTCTTC
BcnI 59 CCGGG CC^[GC]GG
2618 CCCGG
BpuAI 2769 GAAGAC GAAGAC(2/6)
1898 GTCTTC
BsiHKAI 2996 GTGCTC G[AT]GC[AT]^C
3600 GAGCTC
BsmI 134 GAATGC GAATGC(1/-1)
1473 GAATGC
BsoBI 782 CTCGGG C^[CT]CG[GA]G
830 CCCGAG
Bsp1407I 4121 TGTACA T^GTACA
4457 TGTACA
BspHI 737 TCATGA T^CATGA
1575 TCATGA
BspLU11I 218 ACATGT A^CATGT
3536 ACATGT
BsrGI 4121 TGTACA T^GTACA
4457 TGTACA
BstUI 21 CGCG CG^CG
793 CGCG
BstXI 3973 CCACGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4012 CCAGGAGGTTGG
EaeI 1802 TGGCCA [CT]^GGCC[GA]
4009 TGGCCA
HapII 59 CCGG C^CGG
2619 CCGG
HpaII 59 CCGG C^CGG
2619 CCGG
MfeI 1212 CAATTG C^AATTG
3133 CAATTG
MluNI 1802 TGGCCA TGG^CCA
4009 TGGCCA
MscI 1802 TGGCCA TGG^CCA
4009 TGGCCA
MspI 59 CCGG C^CGG
2619 CCGG
MunI 1212 CAATTG C^AATTG
3133 CAATTG
MvnI 21 CGCG CG^CG
793 CGCG
NciI 59 CCGGG CC^[GC]GG
2618 CCCGG
PmaCI 1904 CACGTG CAC^GTG
3974 CACGTG
PmlI 1904 CACGTG CAC^GTG
3974 CACGTG
PstI 1821 CTGCAG CTGCA^G
1919 CTGCAG
RcaI 737 TCATGA T^CATGA
1575 TCATGA
SspBI 4121 TGTACA T^GTACA
4457 TGTACA
SspI 580 AATATT AAT^ATT
3022 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BanI BanII BbvI BclI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HgaI HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII