################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccIII AcsI AgeI Alw44I ApaLI ApoI AscI Asp700I Asp718I AvrII BbeI BbvCI BcgI BciVI BlnI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SalI SbfI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 938 GACGTC GACGT^C AflII 1716 CTTAAG C^TTAAG AflIII 1525 ACATGT A^C[GA][CT]GT AhdI 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3561 CCTCAGG CC^T[ACGT]AGG Aor51HI 4359 AGCGCT AGC^GCT AseI 267 ATTAAT AT^TAAT AsnI 267 ATTAAT AT^TAAT AspEI 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2477 GAGCTC G[AT]GC[AT]^C AspI 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1768 TGCGCA TGC^GCA BamHI 365 GGATCC G^GATCC BbrPI 2550 CACGTG CAC^GTG BfrI 1716 CTTAAG C^TTAAG BglI 3832 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 172 AGATCT A^GATCT BsaBI 3268 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2477 GAGCTC G[AT]GC[AT]^C Bsp1407I 1753 TGTACA T^GTACA BspHI 3153 TCATGA T^CATGA BspLU11I 1525 ACATGT A^CATGT BsrGI 1753 TGTACA T^GTACA BssSI 1815 CTCGTG CACGAG(-5/-1) BstAPI 2546 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3561 CCTCAGG CC^T[ACGT]AGG DraI 1065 TTTAAA TTT^AAA DraIII 2106 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4359 AGCGCT AGC^GCT Eco81I 3561 CCTCAGG CC^T[ACGT]AGG FspI 1768 TGCGCA TGC^GCA HindIII 3513 AAGCTT A^AGCTT MamI 3268 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2499 GCCGGC GCC^GGC NgoMIV 2499 GCCGGC G^CCGGC PflFI 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2550 CACGTG CAC^GTG PmlI 2550 CACGTG CAC^GTG PshAI 1833 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 267 ATTAAT AT^TAAT RcaI 3153 TCATGA T^CATGA SacI 2477 GAGCTC GAGCT^C SapI 501 GAAGAGC GCTCTTC(1/4) ScaI 887 AGTACT AGT^ACT SfiI 3831 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 58 CCCGGG CCC^GGG SphI 3391 GCATGC GCATG^C SspBI 1753 TGTACA T^GTACA SspI 4432 AATATT AAT^ATT Tth111I 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 58 CCCGGG C^CCGGG XmaI 58 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AclI 276 AACGTT AA^CGTT 4473 AACGTT AcyI 938 GACGTC G[GA]^CG[CT]C 2450 GGCGTC AlwNI 328 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 529 CAGCCTCTG ApaI 1780 GGGCCC GGGCC^C 3933 GGGCCC BalI 1981 TGGCCA TGG^CCA 4029 TGGCCA BclI 892 TGATCA T^GATCA 3267 TGATCA BsaHI 938 GACGTC G[GA]^CG[CT]C 2450 GGCGTC BsaI 2357 GAGACC GGTCTC(1/5) 2159 GGTCTC BsmBI 3 GAGACG CGTCTC(1/5) 445 GAGACG BsrFI 2394 ACCGGC [GA]^CCGG[CT] 2499 GCCGGC BstEII 1034 GGTGACC G^GT[ACGT]ACC 1652 GGTGACC BstPI 1034 GGTGACC G^GT[ACGT]ACC 1652 GGTGACC Cfr10I 2394 ACCGGC [GA]^CCGG[CT] 2499 GCCGGC EcoO65I 1034 GGTGACC G^GT[ACGT]ACC 1652 GGTGACC FbaI 892 TGATCA T^GATCA 3267 TGATCA Hin1I 938 GACGTC G[GA]^CG[CT]C 2450 GGCGTC KspI 2170 CCGCGG CCGC^GG 4284 CCGCGG MluNI 1981 TGGCCA TGG^CCA 4029 TGGCCA MscI 1981 TGGCCA TGG^CCA 4029 TGGCCA Psp1406I 276 AACGTT AA^CGTT 4473 AACGTT PvuII 2611 CAGCTG CAG^CTG 2991 CAGCTG SacII 2170 CCGCGG CCGC^GG 4284 CCGCGG StuI 2761 AGGCCT AGG^CCT 3536 AGGCCT Tsp509I 1069 AATT ^AATT 1089 AATT XcmI 2811 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2930 CCATGGGGGTAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XhoII