##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccIII AcsI AgeI Alw44I
ApaLI ApoI AscI Asp700I Asp718I
AvrII BbeI BbvCI BcgI BciVI
BlnI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KasI
KpnI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PvuI RsrII SalI SbfI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 938 GACGTC GACGT^C
AflII 1716 CTTAAG C^TTAAG
AflIII 1525 ACATGT A^C[GA][CT]GT
AhdI 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3561 CCTCAGG CC^T[ACGT]AGG
Aor51HI 4359 AGCGCT AGC^GCT
AseI 267 ATTAAT AT^TAAT
AsnI 267 ATTAAT AT^TAAT
AspEI 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2477 GAGCTC G[AT]GC[AT]^C
AspI 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1768 TGCGCA TGC^GCA
BamHI 365 GGATCC G^GATCC
BbrPI 2550 CACGTG CAC^GTG
BfrI 1716 CTTAAG C^TTAAG
BglI 3832 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 172 AGATCT A^GATCT
BsaBI 3268 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2477 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1753 TGTACA T^GTACA
BspHI 3153 TCATGA T^CATGA
BspLU11I 1525 ACATGT A^CATGT
BsrGI 1753 TGTACA T^GTACA
BssSI 1815 CTCGTG CACGAG(-5/-1)
BstAPI 2546 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3561 CCTCAGG CC^T[ACGT]AGG
DraI 1065 TTTAAA TTT^AAA
DraIII 2106 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 9 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4359 AGCGCT AGC^GCT
Eco81I 3561 CCTCAGG CC^T[ACGT]AGG
FspI 1768 TGCGCA TGC^GCA
HindIII 3513 AAGCTT A^AGCTT
MamI 3268 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2499 GCCGGC GCC^GGC
NgoMIV 2499 GCCGGC G^CCGGC
PflFI 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2550 CACGTG CAC^GTG
PmlI 2550 CACGTG CAC^GTG
PshAI 1833 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 267 ATTAAT AT^TAAT
RcaI 3153 TCATGA T^CATGA
SacI 2477 GAGCTC GAGCT^C
SapI 501 GAAGAGC GCTCTTC(1/4)
ScaI 887 AGTACT AGT^ACT
SfiI 3831 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 58 CCCGGG CCC^GGG
SphI 3391 GCATGC GCATG^C
SspBI 1753 TGTACA T^GTACA
SspI 4432 AATATT AAT^ATT
Tth111I 3731 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 276 AACGTT AA^CGTT
4473 AACGTT
AcyI 938 GACGTC G[GA]^CG[CT]C
2450 GGCGTC
AlwNI 328 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
529 CAGCCTCTG
ApaI 1780 GGGCCC GGGCC^C
3933 GGGCCC
BalI 1981 TGGCCA TGG^CCA
4029 TGGCCA
BclI 892 TGATCA T^GATCA
3267 TGATCA
BsaHI 938 GACGTC G[GA]^CG[CT]C
2450 GGCGTC
BsaI 2357 GAGACC GGTCTC(1/5)
2159 GGTCTC
BsmBI 3 GAGACG CGTCTC(1/5)
445 GAGACG
BsrFI 2394 ACCGGC [GA]^CCGG[CT]
2499 GCCGGC
BstEII 1034 GGTGACC G^GT[ACGT]ACC
1652 GGTGACC
BstPI 1034 GGTGACC G^GT[ACGT]ACC
1652 GGTGACC
Cfr10I 2394 ACCGGC [GA]^CCGG[CT]
2499 GCCGGC
EcoO65I 1034 GGTGACC G^GT[ACGT]ACC
1652 GGTGACC
FbaI 892 TGATCA T^GATCA
3267 TGATCA
Hin1I 938 GACGTC G[GA]^CG[CT]C
2450 GGCGTC
KspI 2170 CCGCGG CCGC^GG
4284 CCGCGG
MluNI 1981 TGGCCA TGG^CCA
4029 TGGCCA
MscI 1981 TGGCCA TGG^CCA
4029 TGGCCA
Psp1406I 276 AACGTT AA^CGTT
4473 AACGTT
PvuII 2611 CAGCTG CAG^CTG
2991 CAGCTG
SacII 2170 CCGCGG CCGC^GG
4284 CCGCGG
StuI 2761 AGGCCT AGG^CCT
3536 AGGCCT
Tsp509I 1069 AATT ^AATT
1089 AATT
XcmI 2811 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2930 CCATGGGGGTAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I Van91I XhoII